| Structure Identification: |
| Journal of Agricultural and Food Chemistry, 2008, 56(4):1164-1171. | | Optimizing Ultraperformance Liquid Chromatographic Analysis of 10 Diterpenoid Compounds in Salvia miltiorrhiza Using Central Composite Design.[Reference: WebLink] |
METHODS AND RESULTS:
A rapid, sensitive, reproducible, and accurate ultraperformance liquid chromatographic (UPLC) method was developed for the simultaneous determination of 10 diterpenoid compounds (tanshinone I, tanshinone IIA, cryptotanshinone, dihydrotanshinone I, 1,2-dihydrotanshinquinone, Methylenetanshinquinone, miltirone, 5,6-dehydrosugiol, sugiol, and przewalskin) in Salvia miltiorrhiza for the first time. Central composite design was applied as a powerful tool to optimize the most dominant parameters that influence the resolution of UPLC, that is, gradient, flow rate, and column temperature. Under optimum conditions, all peaks except 1,2-dihydrotanshinquinone and Methylenetanshinquinone could be baseline separated within 8 min. Furthermore, the contents of these compounds in S. miltiorrhiza samples collected from different provinces of China have also been compared.
CONCLUSIONS:
The results showed that UPLC is one of the most efficient methods for the analysis of diterpenoid compounds in S. miltiorrhiza and that it is a potential method for quality control of this valuable traditional Chinese medicine. |
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