|Source:||The herbs of Epimedium brevicornu|
|Biological Activity or Inhibitors:||1.2"-O-Rhamnosylicariside II shows potent inhibitory activities on osteoclast-like cell formation at a concentration of 2 uM without toxic effects.
2. 2″-O-Rhamnosylicariside II shows potent antioxidant activity, with IC50 values of 11.5 ug/mL and 90.5 uM.
|Solvent:||DMSO, Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.5135 mL||7.5677 mL||15.1355 mL||30.2709 mL||37.8387 mL|
|5 mM||0.3027 mL||1.5135 mL||3.0271 mL||6.0542 mL||7.5677 mL|
|10 mM||0.1514 mL||0.7568 mL||1.5135 mL||3.0271 mL||3.7839 mL|
|50 mM||0.0303 mL||0.1514 mL||0.3027 mL||0.6054 mL||0.7568 mL|
|100 mM||0.0151 mL||0.0757 mL||0.1514 mL||0.3027 mL||0.3784 mL|
Journal of Traditional Medicines, 2007, 24:19-23.
|Inhibitory effect of constituents of Bu-Shen-Jian-Gu-Tang on osteoclast-like cell formation[Reference: WebLink]|
|Chemical investigation of these two fractions resulted in the isolation of 11 compounds, for which the structures were elucidated based on spectroscopic analysis and chemical reactions. The inhibitory activities of the isolated compounds on osteoclast-like cell formation were evaluated. Among the eleven compounds, icariin (1), icarisid II (2), 2''-O-Rhamnosylicariside II (3), kaempferol (8), chikusetsusaponin IVa (10) and chikusetsusaponin V (11) showed potent inhibitory activities at a concentration of 2 μM without toxic effects. The preventive effect of Bu-Shen-Jian-Gu-Tang on bone loss may be due to the inhibitory activities of these compounds.|
Natural Product Sciences, 2008, 14(4):233-8.
|Flavonol Glycosides with Antioxidant Activity from the Aerial Parts of Epimedium koreanum Nakai[Reference: WebLink]|
|The aerial parts of Epimedium koreanum Nakai have been used to stimulate hormone secretion in treating impotence. Ten flavonol glycosides, 3,4,5-trihydroxy-8-prenylflavone 7-O-[β-D-glucopyranosyl(1 → 2)-β-D-glucopyranoside] (1), hyperoside (2), icarisid II (3), 2''-O-Rhamnosylicariside II (4), epimedin A (5), epimedin B (6), epimedin C (7), icariin (8), hexandraside E (9), and epimedoside A (10) were isolated from the an ethylacetate soluble extracts of the aerial parts of Epimedium koreanum Nakai through activity-monitord fractionation and isolation method. The structures of compounds 1 - 10 were elucidated by high resolution fast atom bombardment mass spectrometry and two dimentional nuclear magnetic resonance spectroscopy analysis. Compounds 1 and 4 showed potent antioxidant activity, with IC50 values of 19.7 and 11.5 μg/mL and 88.2 and 90.5 μM, respectively.|