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    3,9-Dihydroxypterocarpan
    Information
    CAS No. 61135-91-9 Price
    Catalog No.CFN97043Purity>=98%
    Molecular Weight256.3 Type of CompoundFlavonoids
    FormulaC15H12O4Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison (Web)
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    Biological Activity
    Description: 3,9-Dihydroxypterocarpan and phaseollidin are all good precursors of the pterocarpan phytoalexin phaseollin.
    In vitro:
    Eur J Biochem. 1984 Jul 2;142(1):127-31.
    Induction of phytoalexin synthesis in soybean. Stereospecific 3,9-dihydroxypterocarpan 6a-hydroxylase from elicitor-induced soybean cell cultures.[Pubmed: 6540173]

    METHODS AND RESULTS:
    A microsomal preparation from elicitor-challenged soybean cell suspension cultures catalyzes an NADPH-dependent and dioxygen-dependent 6a-hydroxylation of 3,9-Dihydroxypterocarpan to 3,6a,9-trihydroxypterocarpan. The latter is a precursor for the soybean phytoalexin glyceollin. No reaction is observed with NADH. The 6a-hydroxylase is inhibited by cytochrome c. Optical rotatory dispersion spectra of the enzymatic product formed from racemic dihydroxypterocarpan and of the remaining unreacted substrate proved that the product has the natural (6aS, 11aS)-configuration and that hydroxylation proceeds with retention of configuration. The 6a-hydroxylase was also found in elicitor-challenged soybean seedlings.
    CONCLUSIONS:
    The results indicate that the 6a-hydroxylase is specifically involved in the biosynthesis of glyceollin.
    3,9-Dihydroxypterocarpan Description
    Source: The rhizomes of Polygonatum kingianum
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
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    Phytomedicine. 2018 Feb 1;40:37-47.
    doi:10.1016/j.phymed.2017.12.030

    PMID: 29496173
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 3.9017 mL 19.5084 mL 39.0168 mL 78.0336 mL 97.5419 mL
    5 mM 0.7803 mL 3.9017 mL 7.8034 mL 15.6067 mL 19.5084 mL
    10 mM 0.3902 mL 1.9508 mL 3.9017 mL 7.8034 mL 9.7542 mL
    50 mM 0.078 mL 0.3902 mL 0.7803 mL 1.5607 mL 1.9508 mL
    100 mM 0.039 mL 0.1951 mL 0.3902 mL 0.7803 mL 0.9754 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Structure Identification:
    Phytochemistry, 1982, 21( 7):1599-603.
    Biosynthesis of the phytoalexin phaseollin in Phaseolus vulgaris[Reference: WebLink]

    METHODS AND RESULTS:
    Feeding experiments in CuCl2-treated French bean (Phaseolus vulgaris) seedlings have demonstrated that labelled 2′,4′,4-trihydroxychalcone, daidzein, 7,2′,4′-trihydroxyisoflavone, 3,9-Dihydroxypterocarpan and phaseollidin are all good precursors of the pterocarpan phytoalexin phaseollin.
    CONCLUSIONS:
    These compounds represent a logical sequence in the biosynthetic pathway to phaseollin.