Science | Nature | Cell | View More
Natural Products
6-Gingerol
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name 6-Gingerol
Price: $50 / 20mg
CAS No.: 23513-14-6
Catalog No.: CFN99931
Molecular Formula: C17H26O4
Molecular Weight: 294.4 g/mol
Purity: >=98%
Type of Compound: Phenols
Physical Desc.: Oil
Source: The rhizomes of Zingber officinale Rosc.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF    Manual
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $8.9 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • Universidade Federal de Santa C... (Brazil)
  • Centrum Menselijke Erfelijkheid (Belgium)
  • Monash University Sunway Campus (Malaysia)
  • University of Bonn (Germany)
  • Uniwersytet Gdański (Poland)
  • University of Stirling (United Kingdom)
  • University of Bordeaux (France)
  • Martin Luther University of Hal... (Germany)
  • Johannes Gutenberg University M... (Germany)
  • Universidad Industrial de Santa... (Colombia)
  • Universite Libre de Bruxelles (Belgium)
  • More...
Package
Featured Products
Canthin-6-one

Catalog No: CFN98730
CAS No: 479-43-6
Price: $318/5mg
Ginsenoside Rk2

Catalog No: CFN92818
CAS No: 364779-14-6
Price: $268/5mg
Epigoitrin

Catalog No: CFN99521
CAS No: 1072-93-1
Price: $100/20mg
Agrimol B

Catalog No: CFN98169
CAS No: 55576-66-4
Price: $138/20mg
Oxypeucedanin

Catalog No: CFN90350
CAS No: 737-52-0
Price: $80/20mg
Phellodendrine

Catalog No: CFN99143
CAS No: 6873-13-8
Price: $90/20mg
Harzianic acid

Catalog No: CFN00118
CAS No: 157148-06-6
Price: $ /
Borneol

Catalog No: CFN98116
CAS No: 507-70-0
Price: $30/20mg
Parishin B

Catalog No: CFN93113
CAS No: 174972-79-3
Price: $80/20mg
Piceatannol

Catalog No: CFN99024
CAS No: 10083-24-6
Price: $40/20mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: 6-Gingerol possesses anti-adipogenic, anti-tumorigenic, anti-invasive, antioxidant, anti-inflammatory, and pro-apoptotic activities, it stimulates apoptosis through upregulation of NAG-1 and G1 cell cycle arrest through downregulation of cyclin D1, multiple mechanisms appear to be involved in 6-gingerol action, including protein degradation as well as β-catenin, PKCε, and GSK-3β pathways. 6-Gingerol can effectively suppress adipogenesis and that it exerts its role mainly through the significant down-regulation of PPARγ and C/EBPα and subsequently inhibits FAS and aP2 expression, also inhibit differentiation in 3T3-L1 cells by attenuating the Akt/GSK3β pathway.
Targets: Wnt/β-catenin | PKC | GSK-3 | MMP(e.g.TIMP) | MAPK | PI3K | NF-kB | STAT | PPAR | ROS | Akt
In vitro:
Phytomedicine. 2013 Apr 15;20(6):481-7.
6-gingerol prevents adipogenesis and the accumulation of cytoplasmic lipid droplets in 3T3-L1 cells.[Pubmed: 23369342 ]
6-Gingerol ((S)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-decanone) is one of the pungent constituents of Zingiber zerumbet (L) Smith (Zingiberaceae family).
METHODS AND RESULTS:
In this study, we investigated the effects of 6-Gingerol on the inhibition of adipogenesis in 3T3-L1 cells. After treatment with 6-Gingerol in differentiation medium for 4 or 8 days, the 3T3-L1 cells were lysed for experimental analysis. Cells were stained with Oil-Red-O to detect oil droplets in adipocytes. The 3T3-L1 cells were lysed and measured for triglyceride contents. The protein expression of adipogenesis-related transcription factor was evaluated by Western blot analysis. 6-Gingerol suppressed oil droplet accumulation and reduced the droplet size in a concentration (5-15 μg/ml)- and time-dependent manner. Treatment of 3T3-L1 cells with 6-Gingerol reduced the protein levels of peroxisome proliferator-activated receptor (PPAR)γ and CCAAT/enhancer-binding protein (C/EBP)α. Additionally, the protein levels of fatty acid synthase (FAS) and adipocyte-specific fatty acid binding protein (aP2) decreased upon treatment with 6-Gingerol. Meanwhile, 6-Gingerol diminished the insulin-stimulated serine phosphorylation of Akt (Ser473) and GSK3β (Ser9).
CONCLUSIONS:
These results suggest that 6-Gingerol effectively suppresses adipogenesis and that it exerts its role mainly through the significant down-regulation of PPARγ and C/EBPα and subsequently inhibits FAS and aP2 expression. 6-Gingerol also inhibited differentiation in 3T3-L1 cells by attenuating the Akt/GSK3β pathway. Our findings provide important insights into the mechanisms underlying the anti-adipogenic activity of 6-Gingerol.
Mol Nutr Food Res. 2010 Nov;54(11):1618-27.
Anti-invasion effects of 6-shogaol and 6-gingerol, two active components in ginger, on human hepatocarcinoma cells.[Pubmed: 20521273]
Hepatocellular carcinoma is the most common type of liver cancer and is highly metastatic. Metastasis is considered to be the major cause of death in cancer patients. Ginger is a natural dietary rhizome with anti-oxidative, anti-inflammatory, and anti-carcinogenic activities. The aims of this study were to evaluate the anti-invasion activity of 6-shogaol and 6-Gingerol, two compounds found in ginger, on hepatoma cells.
METHODS AND RESULTS:
The migratory and invasive abilities of phorbol 12-myristate 13-acetate (PMA)-treated HepG2 and PMA-untreated Hep3B cells were both reduced in a dose-dependent manner by treatment with 6-shogaol and 6-Gingerol. Upon incubation of PMA-treated HepG2 cells and PMA-untreated Hep3B cells with 6-shogaol and 6-Gingerol, matrix metalloproteinase (MMP)-9 activity decreased, whereas the expression of tissue inhibitor metalloproteinase protein (TIMP)-1 increased in both cell types. Additionally, urokinase-type plasminogen activator activity was dose-dependently decreased in Hep3B cells after incubation with 6-shogaol for 24 h. Analysis with semi-quantitative reverse transcription-PCR showed that the regulation of MMP-9 by 6-shogaol and 6-Gingerol and the regulation of TIMP-1 by 6-shogaol in Hep3B cells may on the transcriptional level.
CONCLUSIONS:
These results suggest that 6-shogaol and 6-Gingerol might both exert anti-invasive activity against hepatoma cells through regulation of MMP-9 and TIMP-1 and that 6-shogaol could further regulate urokinase-type plasminogen activity.
Chem Biol Interact. 2010 Apr 15;185(1):12-7.
Genotoxic effect of 6-gingerol on human hepatoma G2 cells.[Pubmed: 20167213 ]
6-Gingerol, a major component of ginger, has antioxidant, anti-apoptotic, and anti-inflammatory activities. However, some dietary phytochemicals possess pro-oxidant effects as well, and the risk of adverse effects is increased by raising the use of doses. The aim of this study was to assess the genotoxic effects of 6-Gingerol and to clarify the mechanisms, using human hepatoma G2 (HepG2) cells.
METHODS AND RESULTS:
Exposure of the cells to 6-Gingerol caused significant increase of DNA migration in comet assay, increase of micronuclei frequencies at high concentrations at 20-80 and 20-40 microM, respectively. These results indicate that 6-Gingerol caused DNA strand breaks and chromosome damage. To further elucidate the underlying mechanisms, we tested lysosomal membrane stability, mitochondrial membrane potential, the intracellular generation of reactive oxygen species (ROS) and reduced glutathione (GSH). In addition, the level of oxidative DNA damage was evaluated by immunocytochemical analysis on 8-hydroxydeoxyguanosine (8-OHdG). Results showed that lysosomal membrane stability was reduced after treatment by 6-Gingerol (20-80 microM) for 40 min, mitochondrial membrane potential decreased after treatment for 50 min, GSH and ROS levels were significantly increased after treatment for 60 min.
CONCLUSIONS:
These suggest 6-Gingerol induces genotoxicity probably by oxidative stress; lysosomal and mitochondrial damage were observed in 6-Gingerol-induced toxicity.
PLoS One . 2014 Aug 26;9(8):e104401.
[6]-Gingerol induces caspase-dependent apoptosis and prevents PMA-induced proliferation in colon cancer cells by inhibiting MAPK/AP-1 signaling[Pubmed: 25157570]
We report mechanism-based evidence for the anticancer and chemopreventive efficacy of [6]-gingerol, the major active principle of the medicinal plant, Ginger (Zingiber officinale), in colon cancer cells. The compound was evaluated in two human colon cancer cell lines for its cytotoxic effect and the most sensitive cell line, SW-480, was selected for the mechanistic evaluation of its anticancer and chemopreventive efficacy. The non-toxic nature of [6]-gingerol was confirmed by viability assays on rapidly dividing normal mouse colon cells. [6]-gingerol inhibited cell proliferation and induced apoptosis as evidenced by externalization of phosphatidyl serine in SW-480, while the normal colon cells were unaffected. Sensitivity to [6]-gingerol in SW-480 cells was associated with activation of caspases 8, 9, 3 &7 and cleavage of PARP, which attests induction of apoptotic cell death. Mechanistically, [6]-gingerol down-regulated Phorbol Myristate Acetate (PMA) induced phosphorylation of ERK1/2 and JNK MAP kinases and activation of AP-1 transcription factor, but had only little effects on phosphorylation of p38 MAP kinase and activation of NF-kappa B. Additionally, it complemented the inhibitors of either ERK1/2 or JNK MAP kinase in bringing down the PMA-induced cell proliferation in SW-480 cells. We report the inhibition of ERK1/2/JNK/AP-1 pathway as a possible mechanism behind the anticancer as well as chemopreventive efficacy of [6]-gingerol against colon cancer.
Food Funct . 2015 Oct;6(10):3334-41.
6-Gingerol modulates proinflammatory responses in dextran sodium sulfate (DSS)-treated Caco-2 cells and experimental colitis in mice through adenosine monophosphate-activated protein kinase (AMPK) activation[Pubmed: 26263169]
Background: 6-Gingerol has been reported to have anti-inflammatory effects in different experimental settings. The present study aimed at evaluating the effect of 6-Gingerol on dextran sodium sulfate (DSS)-induced barrier impairment and inflammation in vitro and in vivo. Methods: a differentiated Caco-2 monolayer was exposed to DSS and treated with different concentrations of 6-Gingerol (0, 1, 5, 10, 50, and 100 μM). Changes in intestinal barrier function were determined using transepithelial electrical resistance (TEER). The anti-inflammatory activity of 6-Gingerol was examined as changes in the expression of proinflammatory cytokine using quantitative real-time PCR. Western blotting was employed to determine the activation of adenosine monophosphate-activated protein kinase (AMPK). Mice with DSS-induced colitis were given different oral dosages of 6-Gingerol daily for 14 days. Body weight and colon inflammation were evaluated, and level of proinflammatory cytokines in colon tissues was measured. Results: 6-Gingerol treatment was shown to restore impaired intestinal barrier function and to suppress proinflammatory responses in DSS-treated Caco-2 monolayers. We found that AMPK was activated on 6-Gingerol treatment in vitro. In animal studies, 6-Gingerol significantly ameliorated DSS-induced colitis by restoration of body weight loss, reduction in intestinal bleeding, and prevention of colon length shortening. In addition, 6-Gingerol suppressed DSS-elevated production of proinflammatory cytokines (IL-1β, TNFα, and IL-12). Conclusion: our findings highlight the protective effects of 6-Gingerol against DSS-induced colitis. We concluded that 6-Gingerol exerts anti-inflammatory effects through AMPK activation. It is suggested that 6-Gingerol has a promising role in treatment of IBD.
6-Gingerol Description
Source: The rhizomes of Zingber officinale Rosc.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
Hosenkoside L

Catalog No: CFN95520
CAS No: N/A
Price: $318/10mg
New compound 19

Catalog No: CFN95538
CAS No: N/A
Price: $413/5mg
Cagayanin

Catalog No: CFN95480
CAS No: 99096-51-2
Price: $318/20mg
Andrographidine A

Catalog No: CFN95210
CAS No: 113963-37-4
Price: $318/10mg
24-Hydroxymomordicine III

Catalog No: CFN95169
CAS No: N/A
Price: $318/5mg
10-Carboxyloganin

Catalog No: CFN95452
CAS No: 182172-02-7
Price: $318/5mg
Quercetin-3'-glucoside

Catalog No: CFN95271
CAS No: 19254-30-9
Price: $318/10mg
Vanillic acid glucoside

Catalog No: CFN95257
CAS No: 32142-31-7
Price: $318/5mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 3.3967 mL 16.9837 mL 33.9674 mL 67.9348 mL 84.9185 mL
5 mM 0.6793 mL 3.3967 mL 6.7935 mL 13.587 mL 16.9837 mL
10 mM 0.3397 mL 1.6984 mL 3.3967 mL 6.7935 mL 8.4918 mL
50 mM 0.0679 mL 0.3397 mL 0.6793 mL 1.3587 mL 1.6984 mL
100 mM 0.034 mL 0.1698 mL 0.3397 mL 0.6793 mL 0.8492 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
Sci Rep. 2015 Mar 2;5:8656.
6-Gingerol reduces Pseudomonas aeruginosa biofilm formation and virulence via quorum sensing inhibition.[Pubmed: 25728862]
Pseudomonas aeruginosa is a well-known pathogenic bacterium that forms biofilms and produces virulence factors via quorum sensing (QS). Interfering with normal QS interactions between signal molecules and their cognate receptors is a developing strategy for attenuating its virulence.
METHODS AND RESULTS:
Here we tested the hypothesis that 6-Gingerol, a pungent oil of fresh ginger, reduces biofilm formation and virulence by antagonistically binding to P. aeruginosa QS receptors. In silico studies demonstrated molecular binding occurs between 6-Gingerol and the QS receptor LasR through hydrogen bonding and hydrophobic interactions. Experimentally 6-Gingerol reduced biofilm formation, several virulence factors (e.g., exoprotease, rhamnolipid, and pyocyanin), and mice mortality. Further transcriptome analyses demonstrated that 6-Gingerol successfully repressed QS-induced genes, specifically those related to the production of virulence factors.
CONCLUSIONS:
These results strongly support our hypothesis and offer insight into the molecular mechanism that caused QS gene repression.
Mol Nutr Food Res. 2012 Aug;56(8):1304-14.
Molecular mechanism inhibiting human hepatocarcinoma cell invasion by 6-shogaol and 6-gingerol.[Pubmed: 22714996]
We previously demonstrated that 6-shogaol and 6-Gingerol, two active compounds in ginger (Zingiber officinale), possess antiinvasive activity against highly metastatic hepatoma cells. The aims of this study were to evaluate the inhibitory effect and molecular mechanism underlying the transcription and translation of matrix metalloproteinases (MMPs) and urokinase-type plasminogen activator (uPA) in Hep3B cells as well as the antiangiogenic activity of 6-Gingerol and 6-shogaol.
METHODS AND RESULTS:
By gelatin zymography and luciferase reporter gene assays, we found that 6-Gingerol and 6-shogaol regulate MMP-2/-9 transcription. Moreover, 6-Gingerol directly decreased expression of uPA, but the 6-shogaol-mediated decrease in uPA was accompanied by up-regulation of plasminogen activator inhibitor (PAI)-1. 6-Gingerol and 6-shogaol concentrations of ≥ 10 μM and ≥ 2.5 μM, respectively, significantly inhibited the phosphorylation of mitogen-activated protein kinase (MAPK) and PI3K/Akt signaling, the activation of NF-κB, and the translocation of NF-κB and STAT3. Incubation of 6-Gingerol or 6-shogaol with human umbilical vein endothelial cells or rat aortas significantly attenuated tube formation.
CONCLUSIONS:
6-Shogaol and 6-Gingerol effectively inhibit invasion and metastasis of hepatocellular carcinoma through diverse molecular mechanisms, including inhibition of the MAPK and PI3k/Akt pathways and NF-κB and STAT3 activities to suppress expression of MMP-2/-9 and uPA and block angiogenesis.
Mol Carcinog. 2008 Mar;47(3):197-208.
Multiple mechanisms are involved in 6-gingerol-induced cell growth arrest and apoptosis in human colorectal cancer cells.[Pubmed: 18058799]
6-Gingerol, a natural product of ginger, has been known to possess anti-tumorigenic and pro-apoptotic activities. However, the mechanisms by which it prevents cancer are not well understood in human colorectal cancer. Cyclin D1 is a proto-oncogene that is overexpressed in many cancers and plays a role in cell proliferation through activation by beta-catenin signaling. Nonsteroidal anti-inflammatory drug (NSAID)-activated gene-1 (NAG-1) is a cytokine associated with pro-apoptotic and anti-tumorigenic properties.
METHODS AND RESULTS:
In the present study, we examined whether 6-Gingerol influences cyclin D1 and NAG-1 expression and determined the mechanisms by which 6-Gingerol affects the growth of human colorectal cancer cells in vitro. 6-Gingerol treatment suppressed cell proliferation and induced apoptosis and G(1) cell cycle arrest. Subsequently, 6-Gingerol suppressed cyclin D1 expression and induced NAG-1 expression. Cyclin D1 suppression was related to inhibition of beta-catenin translocation and cyclin D1 proteolysis. Furthermore, experiments using inhibitors and siRNA transfection confirm the involvement of the PKCepsilon and glycogen synthase kinase (GSK)-3beta pathways in 6-Gingerol-induced NAG-1 expression.
CONCLUSIONS:
The results suggest that 6-Gingerol stimulates apoptosis through upregulation of NAG-1 and G(1) cell cycle arrest through downregulation of cyclin D1. Multiple mechanisms appear to be involved in 6-Gingerol action, including protein degradation as well as beta-catenin, PKCepsilon, and GSK-3beta pathways.
Canthin-6-one

Catalog No: CFN98730
CAS No: 479-43-6
Price: $318/5mg
Ginsenoside Rk2

Catalog No: CFN92818
CAS No: 364779-14-6
Price: $268/5mg
Epigoitrin

Catalog No: CFN99521
CAS No: 1072-93-1
Price: $100/20mg
Agrimol B

Catalog No: CFN98169
CAS No: 55576-66-4
Price: $138/20mg
Oxypeucedanin

Catalog No: CFN90350
CAS No: 737-52-0
Price: $80/20mg
Phellodendrine

Catalog No: CFN99143
CAS No: 6873-13-8
Price: $90/20mg
Harzianic acid

Catalog No: CFN00118
CAS No: 157148-06-6
Price: $ /
Borneol

Catalog No: CFN98116
CAS No: 507-70-0
Price: $30/20mg
Parishin B

Catalog No: CFN93113
CAS No: 174972-79-3
Price: $80/20mg
Piceatannol

Catalog No: CFN99024
CAS No: 10083-24-6
Price: $40/20mg
Tags: buy 6-Gingerol | 6-Gingerol supplier | purchase 6-Gingerol | 6-Gingerol cost | 6-Gingerol manufacturer | order 6-Gingerol | 6-Gingerol distributor