|Description:||1. Dalbergioidin exhibits tyrosinase inhibitory activity with an IC50 of 20 mM.|
2. Dalbergioidin shows a melanin biosynthesis inhibition zone in the culture plate of Streptomyces bikiniensis that has commonly been used as an indicator organism.
3. Dalbergioidin protects MC3T3-E1 osteoblastic cells against H2O2-induced cell damage through activation of the PI3K/AKT/SMAD1 pathway, suggests that it may be useful in bone metabolism diseases, particularly osteoporosis.
4. Dalbergioidin ameliorates doxorubicin-induced renal fibrosis by suppressing the TGF-β signal pathway.
|Targets:||Tyrosinase | TGF-β/Smad | ROS | PI3K | Akt|
|Source:||The roots of Parochetus communis|
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||3.4686 mL||17.343 mL||34.6861 mL||69.3722 mL||86.7152 mL|
|5 mM||0.6937 mL||3.4686 mL||6.9372 mL||13.8744 mL||17.343 mL|
|10 mM||0.3469 mL||1.7343 mL||3.4686 mL||6.9372 mL||8.6715 mL|
|50 mM||0.0694 mL||0.3469 mL||0.6937 mL||1.3874 mL||1.7343 mL|
|100 mM||0.0347 mL||0.1734 mL||0.3469 mL||0.6937 mL||0.8672 mL|
J Microbiol Biotechnol. 2008 May;18(5):874-9.
|Inhibitory effect of dalbergioidin isolated from the trunk of Lespedeza cyrtobotrya on melanin biosynthesis.[Pubmed: 18633284]|
|The active compound was purified from the methanol extract of L cyrtobotrya, followed by several chromatographic methods, and identified as Dalbergioidin (DBG) by spectroscopic methods. The results showed that Dalbergioidin exhibited tyrosinase inhibitory activity with an IC50 of 20 mM. The kinetic analysis tyrosinase inhibition revealed that Dalbergioidin acted as noncompetitive inhibitor. In addition, Dalbergioidin showed a melanin biosynthesis inhibition zone in the culture plate of Streptomyces bikiniensis that has commonly been used as an indicator organism. Furthermore, 27 mM Dalbergioidin decreased more than 50% of melanin contents on the pigmentation using immortalized mouse melanocyte, melan-a cell.|
J Enzyme Inhib Med Chem. 2016;31(sup1):16-22.
|Human neutrophil elastase inhibitory potential of flavonoids from Campylotropis hirtella and their kinetics.[Pubmed: 27558014 ]|
|Campylotropis hirtella is used as a food supplement in the subtropical region of China. In an intensive hunt for human neutrophil elastase inhibitors, we isolated eight flavonoids from C. hirtella three of which (1-3) emerged to be elastase inhibitors. Geranylated flavonoids (1-3) displayed significant inhibitory activity with IC50s between 8.5 and 30.8 μM. The most striking example was geranylated isofavanone 3 that inhibited elastase significantly (IC50 = 30.8 μM) but its parent compound (Dalbergioidin) and isoflavone analog (5) were inactive (IC50 > 200 μM). Compounds (1-3) displayed different kinetic mechanisms (noncompetitive, competitive, and mixed type, respectively) that were dependent upon the parent skeleton. The competitive inhibitor, isoflavan-3-ol-4-one 2 manifested an inhibition of isomerization profile for elastase with kinetic parameters K5 = 0.0386 M-1S-1, K6 = 0.0244 μM-1S-1 and Kiapp = 16.3427 μM. The specific identification of metabolites was accomplished by LC-DAD-ESI/MS that was also used to analyze abundance of active components (1-3) within the plant.|
Mediators Inflamm. 2016;2016:5147571.
|Dalbergioidin Ameliorates Doxorubicin-Induced Renal Fibrosis by Suppressing the TGF-β Signal Pathway.[Pubmed: 28100935]|
|We investigated the effect of Dalbergioidin (DAL), a well-known natural product extracted from Uraria crinita, on doxorubicin- (DXR-) induced renal fibrosis in mice. The mice were pretreated for 7 days with DAL followed by a single injection of DXR (10 mg/kg) via the tail vein. Renal function was analyzed 5 weeks after DXR treatment. DXR caused nephrotoxicity. The symptoms of nephrotic syndrome were greatly improved after DAL treatment. The indices of renal fibrosis, the phosphorylation of Smad3, and the expression of alpha-smooth muscle actin (α-SMA), fibronectin, collagen III (Col III), E-cadherin, TGF-β, and Smad7 in response to DXR were all similarly modified by DAL. The present findings suggest that DAL improved the markers for kidney damage investigated in this model of DXR-induced experimental nephrotoxicity.|
Naunyn Schmiedebergs Arch Pharmacol. 2017 Jul;390(7):711-720.
|Dalbergioidin (DAL) protects MC3T3-E1 osteoblastic cells against H2O2-induced cell damage through activation of the PI3K/AKT/SMAD1 pathway.[Pubmed: 28374099 ]|
|Reactive oxygen species (ROS) is a pivotal pathogenic factor in the development of osteoporosis. Dalbergioidin (DAL) can be isolated from Uraria crinite, an edible herb used as a natural food for childhood skeletal dysplasia. Recent research has implicated DAL as having an antiosteoporosis effect, although the mechanism of this is unclear. We used an effective oxidative stress model, induced by hydrogen peroxide (H2O2) in osteoblastic MC3T3-E1 cells, to investigate the protective effects of DAL in osteoporosis and the underlying molecular mechanisms. The results indicated that treatment with DAL maintained redox balance, reduced MC3T3-E1 cell apoptosis, improved alkaline phosphatase activity, and elevated the osteogenic-related protein expression of Runx2, Osterix, and BMP2 against oxidative damage induced by H2O2. The potential molecular mechanism involved in the protective effect of DAL against H2O2-induced cell death in MC3T3-E1 cells may lie in the activation of the PI3K/AKT/SMAD1 cell signal pathway. Taken together, the results indicated that the potential protective effects of DAL against osteoporosis were linked to a reduction in oxidative damage, suggesting that DAL may be useful in bone metabolism diseases, particularly osteoporosis.|