|Source:||The fruit body of Ganoderma lucidum|
|Biological Activity or Inhibitors:||1. Ganoderenic acid D is most cytotoxic with IC50 values of 0.14 ± 0.01, 0.18 ± 0.02 and 0.26 ± 0.03 mg/mL in Hep G2, Hela and Caco-2 cells, respectively.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.9507 mL||9.7534 mL||19.5069 mL||39.0137 mL||48.7672 mL|
|5 mM||0.3901 mL||1.9507 mL||3.9014 mL||7.8027 mL||9.7534 mL|
|10 mM||0.1951 mL||0.9753 mL||1.9507 mL||3.9014 mL||4.8767 mL|
|50 mM||0.039 mL||0.1951 mL||0.3901 mL||0.7803 mL||0.9753 mL|
|100 mM||0.0195 mL||0.0975 mL||0.1951 mL||0.3901 mL||0.4877 mL|
Natural Product Research, Volume 28, Number 24, 17 December 2014, pp. 2264-2272(9)
|Extraction optimisation and isolation of triterpenoids from Ganoderma lucidum and their effect on human carcinoma cell growth[Reference: WebLink]|
|Following extraction, triterpenoid-enriched fraction was further isolated into 23 fractions, and 7 fractions were identified as ganoderic acids A, B, D, G, H and I and Ganoderenic acid D. Of the seven triterpenoids, Ganoderenic acid D was most cytotoxic with IC50 values of 0.14 ± 0.01, 0.18 ± 0.02 and 0.26 ± 0.03 mg/mL in Hep G2, Hela and Caco-2 cells, respectively. While ganoderic acids A, G and H were relatively non-cytotoxic. The variation of inhibitory effects for these triterpenoids was likely related to their chemical structures.|