Natural Products
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| Source: | The roots of Panax ginseng C. A. Mey. |
| Biological Activity or Inhibitors: | 1. Ginsenoside Rg6 can inhibit JK cell proliferation in human lymphocytoma and induce its apoptosis, the mechanism may through mitochondrial dysfunction and an increase of Bax expression and decrease of Bcl-2 expression. |
| Solvent: | DMSO, Pyridine, Methanol, Ethanol, etc. |
| Storage: | Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com |
| After receiving: | The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling. |
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.PMID: 29328914
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.PMID: 29149595
Scientific Reports 2017 Dec 11;7(1):17332.doi: 10.1038/s41598-017-17427-6.PMID: 29230013
Molecules. 2017 Oct 27;22(11). pii: E1829.doi: 10.3390/molecules22111829.PMID: 29077044
J Cell Biochem. 2018 Feb;119(2):2231-2239.doi: 10.1002/jcb.26385. PMID: 28857247
Phytomedicine. 2018 Feb 1;40:37-47. doi:10.1016/j.phymed.2017.12.030PMID: 29496173| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 1.3046 mL | 6.5232 mL | 13.0463 mL | 26.0926 mL | 32.6158 mL |
| 5 mM | 0.2609 mL | 1.3046 mL | 2.6093 mL | 5.2185 mL | 6.5232 mL |
| 10 mM | 0.1305 mL | 0.6523 mL | 1.3046 mL | 2.6093 mL | 3.2616 mL |
| 50 mM | 0.0261 mL | 0.1305 mL | 0.2609 mL | 0.5219 mL | 0.6523 mL |
| 100 mM | 0.013 mL | 0.0652 mL | 0.1305 mL | 0.2609 mL | 0.3262 mL |
| Citation [1] Molecules. 2013 Jul 9;18(7):8109-19. | Apoptosis-inducing effect of ginsenoside Rg6 on human lymphocytoma JK cells.[Pubmed: 23839115] |
| In this communication our aim was to study the JK cell growth inhibitory and apoptosis-inducing effects of Ginsenoside Rg6 (GRg6) from steamed notoginseng on human lymphocytoma. The CCK-8 method was used to observe the anti-proliferative effect of Ginsenoside Rg6 on human lymphocytoma JK cells. Flow cytometry was performed to analyze the influence of Ginsenoside Rg6 on cell cycle. The Annexin-V FITC/PI double-staining method was used to detect the ratio of apoptotic cells. JC-1 staining was undertaken to observe the influence of Ginsenoside Rg6 on intracellular mitochondrial membrane potential. Finally, western blots were conducted to detect the expression level of apoptosis-related Bax and the Bcl-2 proteins. The results suggested that Ginsenoside Rg6 can inhibit the proliferation of human lymphocytoma JK cells. Ginsenoside Rg6 blocks an S arrest in the cell cycle. With the increase in Ginsenoside Rg6 concentration, the potential in the cell decreased in a dose dependent manner, and Bax protein expression gradually increased, whereas Bcl-2 protein expression gradually decreased. In conclusion, Ginsenoside Rg6 can inhibit JK cell proliferation in human lymphocytoma and induce its apoptosis. The mechanism of action may be related to mitochondrial dysfunction and an increase of Bax expression and decrease of Bcl-2 expression caused by Ginsenoside Rg6 . |
