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    Clinopodiside A
    Information
    CAS No. 142809-89-0 Price $122 / 20mg
    Catalog No.CFN90479Purity>=98%
    Molecular Weight959.12Type of CompoundTriterpenoids
    FormulaC48H78O19Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison (Web)
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    Biological Activity
    Description: Clinopodiside A is a natural product from Clinopodium chinense.
    In vitro:
    Journal of Liquid Chromatography & Related Technologies, Volume 30, Number 4, March 2007, pp. 521-532(12)
    Preparative Isolation and Purification of Saponin and Flavone Glycoside Compounds from Clinopodium chinensis (Benth) O. Kuntze by High-Speed Countercurrent Chromatography[Reference: WebLink]
    A preparative high-speed countercurrent chromatography (HSCCC) method for isolation and purification of didymin, nairutin, and Clinopodiside A from Clinopodium chinensis (Benth) O. Kuntze (Duanxueliu in Chinese) was successfully established.
    METHODS AND RESULTS:
    The separation was performed in two steps with two different kinds of solvent systems. In the first step, ethyl acetate-1-butanol-water (5:0.8:5, v/v) was used as the two-phase solvent system; nairutin was purified, and didymin and Clinopodiside A were eluted together. In the second step, ethyl acetate-methanol-water (5:1:5, v/v) was used as the two-phase solvent system; didymin and Clinopodiside A were separated and purified. After two-step separation, 15.2 mg of nairutin, 39.1 mg of Clinopodiside A, and 20.6 mg of didymin were obtained from 100 mg of crude extract with purities of 96.5%, 98.4%, and 99.1%, respectively, as determined by HPLC analysis. The chemical structures of the three components were confirmed by 1H NMR and 13C NMR.
    Clinopodiside A Description
    Source: The herbs of Clinopodium chinense
    Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.0426 mL 5.2131 mL 10.4262 mL 20.8524 mL 26.0656 mL
    5 mM 0.2085 mL 1.0426 mL 2.0852 mL 4.1705 mL 5.2131 mL
    10 mM 0.1043 mL 0.5213 mL 1.0426 mL 2.0852 mL 2.6066 mL
    50 mM 0.0209 mL 0.1043 mL 0.2085 mL 0.417 mL 0.5213 mL
    100 mM 0.0104 mL 0.0521 mL 0.1043 mL 0.2085 mL 0.2607 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Structure Identification:
    Phytochemistry. 1992 Mar;31(3):1049-50.
    Triterpenoid saponins from Clinopodium polycephalum.[Pubmed: 1368035 ]
    A new triterpenoid saponin, Clinopodiside A, has been isolated from Clinopodium polycephalum.
    METHODS AND RESULTS:
    Its structure was established by spectroscopic methods and X-ray diffraction analysis as 3-O-beta-D-glucopyranosyl(1----6)-[ beta-D-glucopyranosyl (1----4)]-beta-D-glucopyranosyl-olean-11,13(18)-diene-3 beta,16 beta, 23,28-tetrol.