|Description:||1. Gypenoside XLVI shows strong cytotoxic activity against A549 cells, with the IC50 values of 52.63±8.31 ug/ml.|
|Source:||The roots of Panax notoginseng (Burk.) F.H.Chen|
|Solvent:||DMSO, Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.0382 mL||5.191 mL||10.3821 mL||20.7641 mL||25.9551 mL|
|5 mM||0.2076 mL||1.0382 mL||2.0764 mL||4.1528 mL||5.191 mL|
|10 mM||0.1038 mL||0.5191 mL||1.0382 mL||2.0764 mL||2.5955 mL|
|50 mM||0.0208 mL||0.1038 mL||0.2076 mL||0.4153 mL||0.5191 mL|
|100 mM||0.0104 mL||0.0519 mL||0.1038 mL||0.2076 mL||0.2596 mL|
Faseb J., 2014, 28(1).
|Cytotoxic activity of gypenosides and gynogenin from Gynostemma pentaphyllum against A549 cells [Reference: WebLink]|
|By means of chemical and spectroscopic methods, their structures were established as gypenoside LVI, Gypenoside XLVI, gypenoside L, gypenoside LI, damulin A, damulin B and 20S-dammar-24-en-2α, 3β, 12β, 20-tetrol. Gypenoside LVI, Gypenoside XLVI, gypenoside L, gypenoside LI, damulin A, damulin B and 20S-dammar-24-en-2α,3β,12β,20-tetrol all showed strong cytotoxic activity against A549 cells, with the IC50 values of 105.89±2.48, 52.63±8.31, 34.94±4.23, 50.96±9.55, 26.98±0.51, 4.56±0.58, 12.54±0.53 μg/ml respectively, whereas the IC50 value of 20S-Rg3 (positive control) was 55.36±3.20 μg/ml. Comparing their structures with cytotoxic activity, these compounds might possess some kind of structure-activity relationship. Based on the structure and cytotoxic activity relationships of these seven constituents, the OH group in C-2, the double bond in C20(21) and C20(22) positions, the connected sugar number and the configuration in C-20 were important for cytotoxic activity against A549 cells.|