|Source:||The herbs of Eclipta prostrata|
|Biological Activity or Inhibitors:|
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||3.3309 mL||16.6545 mL||33.3089 mL||66.6178 mL||83.2723 mL|
|5 mM||0.6662 mL||3.3309 mL||6.6618 mL||13.3236 mL||16.6545 mL|
|10 mM||0.3331 mL||1.6654 mL||3.3309 mL||6.6618 mL||8.3272 mL|
|50 mM||0.0666 mL||0.3331 mL||0.6662 mL||1.3324 mL||1.6654 mL|
|100 mM||0.0333 mL||0.1665 mL||0.3331 mL||0.6662 mL||0.8327 mL|
Yao Xue Xue Bao. 2001 Jan;36(1):34-7.
|Studies on the chemical constituents of Eclipta prostrata (L).[Pubmed: 12579857]|
|To study the chemical constituents of Eclipta prostrata (L). METHODS: The constituents of E. prostrata were systematically separated with the Bohlmann method and percolation and hot extraction methods, and various chromatographies. The structures were elucidated by chemical and spectroscopic means. RESULTS: Ten compounds were isolated from the Eclipta prostrata. Their structures were determined as wedelolactone (1), demethylwedelolactone (2), Isodemethylwedelolactone (3), alpha-formylterthienyl (4), strychnolactone (5), beta-sitosterol (6), nonacosanol (7), stearic acid (8), lacceroic acid (9), 3,4-dihydoxy benzoic acid (10). Fourteen ocmpounds, including hydrocarbons and its esters were identified by GC-MS from the least polar fractions. CONCLUSION: Compound 3 is a new coumestan named Isodemethylwedelolactone. Compounds 2-10 and compounds characterized by GC-MS analysis were obtained for the first time from Eclipta prostrata.|
Se Pu. 2007 May;25(3):371-3.
|Simultaneous determination of wedelolactone and isodemethylwedelolactone in Herba ecliptae by reversed-phase high performance liquid chromatography.[Pubmed: 17679432]|
|A reversed-phase high performance liquid chromatographic method (RP-HPLC) was developed for the simultaneous determination of wedelolactone and Isodemethylwedelolactone in Herba Ecliptae from different sources. The separation was performed on a Kromasil C18 column (250 mm x 4.6 mm, 5 microm), with methanol-0.5% acetic acid (55 45, v/v) as mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was set at 351 nm and the volume of injection was 20 microL. There were good linear relationships between the mass concentrations and the peak areas of Isodemethylwedelolactone and wedelolactone in the ranges of 1.6 - 32.0 mg/L (r = 0.999 5) and 5.6 - 112.0 mg/L (r = 0.999 8), respectively. The recoveries were found to be in the range of 97.5% - 98.2% and 99.0% - 100.2%, respectively. The results of the experiments have demonstrated that the established method is rapid and simple with good accuracy and reproducibility for the simultaneous determination of wedelolactone and Isodemethylwedelolactone. The method is suitable for the quality control of Herba Ecliptae from different sources.|