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    Isovitexin 2''-O-arabinoside
    Isovitexin 2''-O-arabinoside
    CAS No. 53382-71-1 Price $268 / 5mg
    Catalog No.CFN90943Purity>=98%
    Molecular Weight564.49Type of CompoundFlavonoids
    FormulaC26H28O14Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison (Web)
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    Biological Activity
    Description: Isovitexin 2''-O-arabinoside is a natural product from Secale cereale.
    In vitro:
    Journal of Plant Physiology, 1988, 133(2):178-182.
    Biochemical and Immunological Characterization of Chalcone Synthase from Rye Leaves.[Reference: WebLink]
    As part of investigations on the role and the location of the key enzyme of flavonoid biosynthesis, chalcone synthase (CHS) in leaf tissues of Gramineae, CHS from rye (Secale cereale L.) was purified and characterized biochemically and immunologically.
    Km-values for the substrates p-coumaroyl-CoA and caffeoyl-CoA were 0.6 and 1.45 μM, respectively, the corresponding Km-values for malonyl-CoA were 1.4 and 1.5 μM. The pH-optimum for the formation of 2′,4,4′,6′-tetrahydroxychalcone was 8 and for the formation of 2′,3,4,4′,6′-pentahydroxychalcone, 6.5. A 50% reduction in enzyme activity was caused by 9 μM apigenin, 13 μM luteolin, 36 μM CoA, 45 μM naringenin, 45 μM eriodictyol, and 62 μM Isovitexin 2''-O-arabinoside. SDS-PAGE analysis of the purified enzyme revealed two peptides with apparent molecular weights of 43 and 44 kd, respectively.
    On western blots run with the purified enzyme as well as with crude enzyme extracts, both peptides were immunostained by polyclonal antibodies raised against rye or parsley CHS. The recognition of these peptides by single monoclonal antibodies demonstrated that both peptides were similar and derived from CHS. Applied to western blots run with CHS-containing extracts of parsley, spinach, pea, maize, and oat, the same antibodies labelled only a single peptide, or in some cases probably even more than two peptides.
    Isovitexin 2''-O-arabinoside Description
    Source: The leaves of Secale cereale.
    Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.7715 mL 8.8576 mL 17.7151 mL 35.4302 mL 44.2878 mL
    5 mM 0.3543 mL 1.7715 mL 3.543 mL 7.086 mL 8.8576 mL
    10 mM 0.1772 mL 0.8858 mL 1.7715 mL 3.543 mL 4.4288 mL
    50 mM 0.0354 mL 0.1772 mL 0.3543 mL 0.7086 mL 0.8858 mL
    100 mM 0.0177 mL 0.0886 mL 0.1772 mL 0.3543 mL 0.4429 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Structure Identification:
    Phytochemistry, 1983, 22(11):2627-2628.
    Two isovitexin 2″-O-glycosides from primary leaves of Secale cereale.[Reference: WebLink]
    Two isovitexin 2″-O-glycosides have been isolated from primary leaves of rye and identified as Isovitexin 2''-O-arabinoside and isovitexin 2.