|Cancer Lett. 2007 Aug 18;253(2):224-35. |
|Leachianone A as a potential anti-cancer drug by induction of apoptosis in human hepatoma HepG2 cells.[Pubmed: 17379399]|
|The Chinese herbal medicine Radix Sophorae is widely applied as an anti-carcinogenic/ anti-metastatic agent against liver cancer. |
METHODS AND RESULTS:
In this study, Leachianone A, isolated from Radix Sophorae, possessed a profound cytotoxic activity against human hepatoma cell line HepG2 in vitro, with an IC(50) value of 3.4microg/ml post-48-h treatment. Its action mechanism via induction of apoptosis involved both extrinsic and intrinsic pathways. Its anti-tumor effect was further demonstrated in vivo by 17-54% reduction of tumor size in HepG2-bearing nude mice, in which no toxicity to the heart and liver tissues was observed.
In conclusion, this is the first report describing the isolation of Leachianone A from Radix Sophorae and the molecular mechanism of its anti-proliferative effect on HepG2 cells.
|Journal of Huazhong Normal University, 2014, 48(4):520-4. |
|Lavandulyl flavonoids with sodium-dependent glucose cotransporter 2 inhibitory activity from Sophora flavescens.[Reference: WebLink]|
|To discover new bioactive Sodium-dependent glucose cotransporter 2(SGLT2)inhibitors from the traditional Chinese medicine"Ku Shen"(roots of Sophora flavescens),the bioassay-guided purification of an active ethyl acetate fraction was performed.
METHODS AND RESULTS:
Sixteen lavandulyl flavonoids were isolated and their structures were elucidated as kushenol H(1),kushenol K(2),kurarinol(3),kushenol Y(4),kushenol P(5),norkurarinone(6),kushenol I(7),kushenol N(8),(-)-kurarinone(9),kushenol X(10),neokurarinol(11),kushenol C(12),sophoraflavanone G(13),Leachianone A(14),kuraridine(15)and kushenol A(16).All isolated compounds exhibited inhibitory activity against SGLT2.Among them,the two main constituents of the active EtOAc fraction,(-)-kurarinone(9)and sophoraflavanone G(13)showed the most potential inhibitory activity against SGLT2with the IC50values of 2.24μmol/L and 1.45μmol/L,respectively.