|Source:||The roots of Sophora flavescens|
|Biological Activity or Inhibitors:||1. Leachianone A possesses a profound cytotoxic activity against human hepatoma cell line HepG2 in vitro, with an IC(50) value of 3.4microg/ml post-48-h treatment.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.2805 mL||11.4025 mL||22.805 mL||45.61 mL||57.0125 mL|
|5 mM||0.4561 mL||2.2805 mL||4.561 mL||9.122 mL||11.4025 mL|
|10 mM||0.2281 mL||1.1403 mL||2.2805 mL||4.561 mL||5.7013 mL|
|50 mM||0.0456 mL||0.2281 mL||0.4561 mL||0.9122 mL||1.1403 mL|
|100 mM||0.0228 mL||0.114 mL||0.2281 mL||0.4561 mL||0.5701 mL|
Cancer Lett. 2007 Aug 18;253(2):224-35.
|Leachianone A as a potential anti-cancer drug by induction of apoptosis in human hepatoma HepG2 cells.[Pubmed: 17379399]|
|The Chinese herbal medicine Radix Sophorae is widely applied as an anti-carcinogenic/ anti-metastatic agent against liver cancer. In this study, Leachianone A, isolated from Radix Sophorae, possessed a profound cytotoxic activity against human hepatoma cell line HepG2 in vitro, with an IC(50) value of 3.4microg/ml post-48-h treatment. Its action mechanism via induction of apoptosis involved both extrinsic and intrinsic pathways. Its anti-tumor effect was further demonstrated in vivo by 17-54% reduction of tumor size in HepG2-bearing nude mice, in which no toxicity to the heart and liver tissues was observed. In conclusion, this is the first report describing the isolation of Leachianone A from Radix Sophorae and the molecular mechanism of its anti-proliferative effect on HepG2 cells.|