ChemFaces is a professional high-purity natural products manufacturer.
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More articles cited ChemFaces products.
Appl Microbiol Biotechnol. 2015 Dec 21. Biofactors.2017 Oct 24.Food Analytical Methods07 April 2017Int J Mol Sci. 2018 Jan 23;Tea Res. Ins. Of ChinaJuly 13, 2017;
Int J Mol Sci. 2017 Dec 21;The Japan Society for Analy. Chem.2017 Nov. 8;Jour. of Stored Pro & Postharvest Res.March 2016Polytechnic University of Catalonia2016SBRAS2016(12)
J Agric Food Chem. 2017 Apr 5Hindawi Publishing Corporation2015 July 30Chemistry of Natural CompoundsJan. 2018;J Cell Biochem.2018 Feb;
Our products had been exported to the following research institutions and universities, And still growing.
University Medical Center Mainz (Germany)Mendel University in Brno (Czech Republic)Pennsylvania State University (USA)Almansora University (Egypt)
University of Maryland (USA)Chiang Mai University (Thailand)Chang Gung University (Taiwan)University of Illinois at Chicago (USA)
University of Ioannina (Greece)Korea Food Research Institute(KF... (Korea)Ain Shams University (Egypt)
||Aristolochic acid A is a potent nephrotoxin, which strongly induced toxic damage during ovarian maturation by inhibiting Akt phosphorylation-mediated suppression of apoptosis. |
||Bcl-2/Bax | Caspase | PARP | Akt|
|Chem Res Toxicol. 2014 Dec 15;27(12):2128-35. |
|Aristolochic Acid A induces ovarian toxicity by inhibition of akt phosphorylation.[Pubmed: 25406029]|
|Aristolochic acids are natural products found in Chinese herbs of the Aristolochiaceae family. Aristolochic acid I (Aristolochic acid A,AAI) is a potent carcinogen and was found to be toxic in animal and clinical studies. Apoptosis is a rapid, selective process of physiological cell deletion that regulates the balance between cell proliferation and cell death and is induced by various kinds of damage. However, the toxicity of AAI during ovarian maturation in the mouse is unclear and is the subject of the present investigation.
METHODS AND RESULTS:
We used Chinese hamster ovary-K1 (CHO-K1) cells and an AAI injection mouse model: MTT assay was used to assess AA toxicity to cells; ovary size and weight were measured to determine the toxicity of AA to mouse ovary; western blot was used to assess apoptosis; TUNEL assay was used to evaluate apoptotic cell death; and immunohistochemistry was used to examine the local expression of apoptotic proteins in ovary tissue. We found that AAI significantly inhibits the viability of CHO-K1 cells and strongly induces apoptotic cell death in CHO-K1 cells and in mouse ovary. In addition, we observed that AAI markedly increases the expression of pro-apoptotic proteins, including Bax, caspase-3, caspase-9, and poly(ADP) ribose polymerase (PARP). In contrast, anti-apoptotic proteins, such as Bcl-2 and survivin, were decreased by AAI treatment. Furthermore, we observed that ovary size and weight were significantly reduced and that the number of ovulated oocytes was markedly suppressed in AAI-treated mice.
These results suggest that AAI strongly induces toxic damage during ovarian maturation by inhibiting Akt phosphorylation-mediated suppression of apoptosis.
|Zhong Yao Cai. 2010 Aug;33(8):1228-33. |
|The determination of aristolochic acid A in different processed Aristolochia manshuriensis and the test of influence about renal function in rats.[Pubmed: 21213532]|
|To study and approach the processing methods and mechanism which can markedly reduce the content of aristolochic acid in Aristolochia manshuriensis and lighten the nephrotoxicity of aristolochic acid.
METHODS AND RESULTS:
A traditional "attenuation" processing method was used and 30 types of samples which contain one crude and 29 types of processed sample were obtained. The contents of Aristolochic acid A in every sample were determined by HPLC. According to the Rat's acute renal injury test, the influence of animal's renal function was investigated for representative samples.
The content of aristolochic acid in six types of samples depressed markedly (30% or more depressed) which processing with boiling in the limewater, steaming with limewater, boiling in the juice of liquorice, boiling in the decoction of black soybean, boiling in the soda water and stir-baked with talcum powder, the content of aristolochic acid in other processed samples also depressed with a large discrepancy. The toxicology test results showed that the above-mentioned 6 samples all can relieve renal injury of rats. There could be some associativity between the degree of renal injury relieving and the content of Aristolochic acid A in the samples.
The content of aristolochic acid can be reduced and the nephrotoxicity for animals can be lightened with some eligible processing methods for the traditional Chinese medicines containing aristolochic acid with the representative of Aristolochia manshuriensis.
Aristolochic acid A Description
||The herbs of Aristolochia debilis Sieb. et Zucc.
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Recent ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.PMID: 29328914
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.PMID: 29149595
Scientific Reports 2017 Dec 11;7(1):17332.doi: 10.1038/s41598-017-17427-6.PMID: 29230013
Molecules. 2017 Oct 27;22(11). pii: E1829.doi: 10.3390/molecules22111829.PMID: 29077044
J Cell Biochem. 2018 Feb;119(2):2231-2239.doi: 10.1002/jcb.26385. PMID: 28857247
Phytomedicine. 2018 Feb 1;40:37-47. doi:10.1016/j.phymed.2017.12.030PMID: 29496173
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* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.