|Source:||The bark of Magnolia obovata|
|Biological Activity or Inhibitors:|| 1. Magnolianin can inhibit strongly (IC50 = 0.45 uM) 5-HETE formation by die cytosol of guinea pig polymorphonuclear leukocytes.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.2092 mL||6.0459 mL||12.0919 mL||24.1838 mL||30.2297 mL|
|5 mM||0.2418 mL||1.2092 mL||2.4184 mL||4.8368 mL||6.0459 mL|
|10 mM||0.1209 mL||0.6046 mL||1.2092 mL||2.4184 mL||3.023 mL|
|50 mM||0.0242 mL||0.1209 mL||0.2418 mL||0.4837 mL||0.6046 mL|
|100 mM||0.0121 mL||0.0605 mL||0.1209 mL||0.2418 mL||0.3023 mL|
Chem Pharm Bull (Tokyo). 2001 Jun;49(6):716-20.
|Effects of constituents from the bark of Magnolia obovata on nitric oxide production in lipopolysaccharide-activated macrophages.[Pubmed: 11411523]|
|The methanolic extract from a Japanese herbal medicine, the bark of Magnolia obovata, was found to inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-activated macrophages. By bioassay-guided separation, three neolignans (magnolol, honokiol, obovatol) and three sesquiterpenes (alpha-eudesmol, beta-eudesmol, gamma-eudesmol) were obtained as active constituents. A trineolignan (Magnolianin), a phenylpropanoid glycoside (syringin), lignan glycosides (liriodendrin, (+)-syringaresinol 4'-O-beta-D-glucopyranoside) and a sesquiterpene (caryophyllene oxide) did not show any activity.|
Tetrahedron Letters, 1993 , 34 (6) :1051-1054.
|Structure of Magnolianin, a novel trilignan possessing potent 5-lipoxygenase inhibitory activity[Reference: WebLink]|
|Magnolianin, a novel trilignan with a 1, 4-benzodioxane ring, isolated from the bark of Magnolia obovata, has been assigned structure on the basis of extensive analysis of 2D 1H and 13C NMR of its triacetate derivative. Magnolianin has been found to inhibit strongly (IC50 = 0.45 μM) 5-HETE formation by die cytosol of guinea pig polymorphonuclear leukocytes.|