|Source:||The herbs of Nerium indicum Mill.|
|Biological Activity or Inhibitors:||1. Neritaloside is a cardiac glycoside.
2. Neritaloside exhibits central nervous system depressant activity in mice at a dose of 25 mg/kg.
|Solvent:||DMSO, Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.6872 mL||8.436 mL||16.8719 mL||33.7439 mL||42.1799 mL|
|5 mM||0.3374 mL||1.6872 mL||3.3744 mL||6.7488 mL||8.436 mL|
|10 mM||0.1687 mL||0.8436 mL||1.6872 mL||3.3744 mL||4.218 mL|
|50 mM||0.0337 mL||0.1687 mL||0.3374 mL||0.6749 mL||0.8436 mL|
|100 mM||0.0169 mL||0.0844 mL||0.1687 mL||0.3374 mL||0.4218 mL|
Phytochemistry. 1999 Feb;50(3):435-8.
|Bio-active cardenolides from the leaves of Nerium oleander.[Pubmed: 9933955]|
|A bioactivity directed isolation of the methanolic extract of the fresh, uncrushed leaves of Nerium oleander showing a central nervous system (CNS) depressant effect in mice has been undertaken. As a result, four CNS depressant cardenolides including a new cardenolide, neridiginoside and three known constituents, nerizoside, Neritaloside and odoroside-H, have been isolated which exhibited CNS depressant activity in mice at a dose of 25 mg/kg.|
J Ethnopharmacol. 2011 Apr 12;134(3):781-8.
|Characterization of the anticancer properties of monoglycosidic cardenolides isolated from Nerium oleander and Streptocaulon tomentosum.[Pubmed: 21291990]|
|Bioactivity guided fractionation of Breastin resulted in the isolation of the monoglycosidic cardenolides oleandrine, oleandrigeninsarmentoside, Neritaloside, odoroside H, and odoroside A (IC(50)-values between 0.010 and 0.071 μg/ml). CONCLUSIONS: The observed anticancer activities of extracts and isolated cardenolides are in agreement with the ethnomedicinal use of Streptocaulon tomentosum and Nerium oleander. The most active anticancer compounds from both species are monoglycosidic cardenolides possessing the 3β,14β-dihydroxy-5β-card-20(22)-enolide structure with or without an acetoxy group at C-16. The results indicate that the cytotoxic effects are induced by the inhibition of the plasma membrane bound Na(+)/K(+)-ATPase.|
Anal Chem. 2000 Aug 1;72(15):3547-52.
|LC/MS/MS analyses of an oleander extract for cancer treatment.[Pubmed: 10952541]|
|An HPLC/MS/MS method has been developed for the characterization and quantification of the cardiac glycosides oleandrin, odoroside, Neritaloside and the aglycone oleandrigenin, all contained in a patented-hot-water extract of Nerium oleander L (Anvirzel). Qualitative analysis of such extracts was achieved using a hybrid tandem quadrupole time-of-flight (QqTOF) mass spectrometer. Collision-induced dissociation (CID) mass spectra of oleandrin, oleandrigenin, odoroside, and Neritaloside were obtained with greater than 5 ppm mass accuracy and resolution routinely in excess of 8000 (fwhm). The detection limit for oleandrin of 20 pg (injected) was realized when the precursor-to-product ion transition, m/z 577 --> 373, was monitored. We have also applied the analytical method to the determination of oleandrin, oleandrigenin, Neritaloside, and odoroside in human plasma following an intramuscular injection of Anvirzel.|