|Source:||The herbs of Peucedanum decursivum Maxim.|
|Biological Activity or Inhibitors:||1. Nodakenetin has clinical efficacy.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||4.0601 mL||20.3004 mL||40.6009 mL||81.2018 mL||101.5022 mL|
|5 mM||0.812 mL||4.0601 mL||8.1202 mL||16.2404 mL||20.3004 mL|
|10 mM||0.406 mL||2.03 mL||4.0601 mL||8.1202 mL||10.1502 mL|
|50 mM||0.0812 mL||0.406 mL||0.812 mL||1.624 mL||2.03 mL|
|100 mM||0.0406 mL||0.203 mL||0.406 mL||0.812 mL||1.015 mL|
Biomed Chromatogr. 2010 Feb;24(2):216-21.
|A new metabolite of nodakenetin by rat liver microsomes and its quantification by RP-HPLC method.[Pubmed: 19572262]|
|The biotransformation of Nodakenetin (NANI) by rat liver microsomes in vitro was investigated. Two major polar metabolites were produced by liver microsomes from phenobarbital-pretreated rats and detected by reversed-phase high-performance liquid chromatography (RP-HPLC) analysis. The chemical structures of two metabolites were firmly identified as 3'(R)-hydroxy-Nodakenetin-3'-ol and 3'(S)-hydroxy-Nodakenetin-3'-ol, respectively, on the basis of their (1)H-NMR, MS and optical rotation analysis. The latter was a new compound. A sensitive, selective and simple RP-HPLC method has been developed for the simultaneous determination of Nodakenetin and its two major metabolites in rat liver microsomes. Chromatographic conditions comprise a C(18) column, a mobile phase with MeOH-H(2)O (40 : 60, v/v), a total run time of 40 min, and ultraviolet absorbance detection at 330 nm. In the rat heat-inactivated liver microsomal supernatant, the lower limits of detection and quantification of metabolite I, metabolite II and Nodakenetin were 5.0, 2.0, 10.0 ng/mL and 20.0, 5.0, 50.0 ng/mL, respectively, and their calibration curves were linear over the concentration range 50-400, 20-120 and 150-24000 ng/mL, respectively. The results provided a firm basis for further evaluating the pharmacokinetics and clinical efficacy of Nodakenetin.|