|Source:||The bark of Eucommia ulmoides|
|Biological Activity or Inhibitors:||1. Pinoresinol diglucoside is a putative α-glucosidase inhibiting compound.
2. Pinoresinol diglucoside is a important antihypertensive compound.
|Solvent:||DMSO, Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.4648 mL||7.3242 mL||14.6484 mL||29.2967 mL||36.6209 mL|
|5 mM||0.293 mL||1.4648 mL||2.9297 mL||5.8593 mL||7.3242 mL|
|10 mM||0.1465 mL||0.7324 mL||1.4648 mL||2.9297 mL||3.6621 mL|
|50 mM||0.0293 mL||0.1465 mL||0.293 mL||0.5859 mL||0.7324 mL|
|100 mM||0.0146 mL||0.0732 mL||0.1465 mL||0.293 mL||0.3662 mL|
Chem Biodivers. 2013 Jul;10(7):1322-7.
|Heat shock factor 1 inducers from the bark of Eucommia ulmoides as cytoprotective agents.[Pubmed: 23847077]|
|The barks of Eucommia ulmoides (Eucommiae Cortex, Eucommiaceae) have been used as a traditional medicine in Korea, Japan, and China to treat hypertension, reinforce the muscles and bones, and recover the damaged liver and kidney functions. Among these traditional uses, to establish the recovery effects on the damaged organs on the basis of phytochemistry, the barks of E. ulmoides have been investigated to afford three known phenolic compounds, coniferaldehyde glucoside (1), bartsioside (2), and feretoside (3), which were found in the family Eucommiaceae for the first time. The compounds 1-3 were evaluated for their inducible activities on the heat shock factor 1 (HSF1), and heat shock proteins (HSPs) 27 and 70, along with four compounds, geniposide (4), geniposidic acid (5), Pinoresinol diglucoside (6), and liriodendrin (7), which were previously reported from E. ulmoides. Compounds 1-7 increased expression of HSF1 by a factor of 1.214, 1.144, 1.153, 1.114, 1.159, 1.041, and 1.167 at 3 μM, respectively. Coniferaldehyde glucoside (1) showed the most effective increase of HSF1 and induced successive expressions of HSP27 and HSP70 in a dose-dependent manner without cellular cytotoxicity, suggesting a possible application as a HSP inducer to act as cytoprotective agent.|
Appl Microbiol Biotechnol. 2012 Feb;93(4):1475-83.
|Structure identification and fermentation characteristics of pinoresinol diglucoside produced by Phomopsis sp. isolated from Eucommia ulmoides Oliv.[Pubmed: 22048615]|
|Pinoresinol diglucoside (PDG) is the important antihypertensive compound in Eucommia ulmoides Oliv., a traditional Chinese herb medicine. The research objective was to certify the possibility of producing PDG through fermentation. PDG-producing endophytic fungi were isolated from E. ulmoides Oliv., and the highest PDG-yielding (11.65 mg/L) isolate, XP-8, was identified as Phomopsis sp. according to the morphological characteristics and the phylogenetic tree constructed on the basis of the gene sequence in the internal transcribed spacers district. During the fermentation, PDG production outside cells starts at the later stage of cell growth when the residual sugar in the medium was low. The study reveals the possibility for production of PDG by fermentation.|
Chem Pharm Bull (Tokyo). 2003 May;51(5):508-15.
|Biotransformation of pinoresinol diglucoside to mammalian lignans by human intestinal microflora, and isolation of Enterococcus faecalis strain PDG-1 responsible for the transformation of (+)-pinoresinol to (+)-lariciresinol.[Pubmed: 12736449]|
|By anaerobic incubation of Pinoresinol diglucoside (1) from the bark of Eucommia ulmoides with a fecal suspension of humans, eleven metabolites were formed, and their structures were identified as (+)-pinoresinol (2), (+)-lariciresinol (3), 3'-demethyl-(+)-lariciresinol (4), (-)-secoisolariciresinol (5), (-)-3-(3", 4"-dihydroxybenzyl)-2-(4'-hydroxy-3'-methoxybenzyl)butane-1, 4-diol (6), 2-(3', 4'-dihydroxybenzyl)-3-(3", 4"-dihydroxybenzyl)butane-1, 4-diol (7), etc. by various spectroscopic means, including two dimensional (2D)-NMR, mass spectrometry and circular dichroism. A possible metabolic pathway was proposed on the basis of their structures and time course experiments monitored by thin-layer chromatography. Furthermore, a bacterial strain responsible for the transformation of (+)-pinoresinol to (+)-lariciresinol was isolated from a human fecal suspension and identified as Enterococcus faecalis strain PDG-1.|
J. Korean Soc. Appl. Bi., 2014, 57(4):473-9.
|Pinoresinol Diglucoside is Screened as a Putative alpha-Glucosidase Inhibiting Compound in Actinidia arguta leaves[Reference: WebLink]|
|Actinidia arguta leaves are consumed as a popular food material in Korea and have been reported to exert beneficial effects on humans due to its constituent polyphenolic compounds. In this study, the alpha-glucosidase inhibitory compounds in A. arguta were screened and identified through alpha-glucosidase-guided fractionation and metabolomic analysis. The 50% ethanol extracts of A. arguta showed strong inhibitory effect (32.6%), which was comparable to acarbose as a positive control (30.0%). Through multiple steps of fractionation, Pinoresinol diglucoside and fertaric acid were identified as the major potent compounds in A. arguta inhibiting alpha-glucosidase activity by liquid chromatography mass spectrometry analysis and metabolomic comparison. Particularly, because pinoresinol and its glycosides have been demonstrated as alpha-glucosidase inhibitory agents, Pinoresinol diglucoside was proposed to be a putative key compound for alpha-glucosidase inhibition in A. arguta. This is the first study demonstrating the anti-diabetic effect of a pinoresinol-containing fraction of A. arguta and would be useful for its application as a natural alpha-glucosidase inhibitor.|