|Source:||The barks of Rauvolfia yunnanensis Tsiang|
|Biological Activity or Inhibitors:||1. Robinin has protective effect against the ox-LDL induced inflammation stress in hPBMCs by inhibiting TLR4-NF-κB signaling pathway.
2. Robinin has cardioprotective effect on doxorubicin-induced cardiac toxicity by modulating TGF-β1 signaling pathway in Sprague Dawley rats.
|Solvent:||Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.3501 mL||6.7504 mL||13.5007 mL||27.0015 mL||33.7519 mL|
|5 mM||0.27 mL||1.3501 mL||2.7001 mL||5.4003 mL||6.7504 mL|
|10 mM||0.135 mL||0.675 mL||1.3501 mL||2.7001 mL||3.3752 mL|
|50 mM||0.027 mL||0.135 mL||0.27 mL||0.54 mL||0.675 mL|
|100 mM||0.0135 mL||0.0675 mL||0.135 mL||0.27 mL||0.3375 mL|
Int Immunopharmacol. 2014 Jan;18(1):191-7.
|Robinin modulates TLR/NF-κB signaling pathway in oxidized LDL induced human peripheral blood mononuclear cells.[Pubmed: 24295649]|
|This study was designed to investigate whether Robinin administration modulates toll-like receptor (TLR) and nuclear factor-kappa B (NF-κB) signaling pathway in oxidized LDL induced human peripheral blood mononuclear cells (hPBMCs). The hPBMCs were isolated from healthy human volunteers and the cells were cultured in collagen coated plates at 37°C with 5% CO2 and RPMI as culture medium and were grouped Robinin inhibited the ox-LDL induced TLR2 and TLR4 expression at mRNA level and inhibited the translocation of NF-κB p65 by modulating the TLR-NF-κB signaling pathway thereby inhibiting cytokine production and down regulated inflammatory enzymes like cyclooxygenase (COX), lipoxygenase (LOX), nitric oxide synthase (NOS) and prostaglandin E2 (PGE2), thus having protective effect against the ox-LDL induced inflammation stress in hPBMCs by inhibiting TLR4-NF-κB signaling pathway.|
Biomed Pharmacother. 2014 Oct;68(8):989-98.
|Robinin modulates doxorubicin-induced cardiac apoptosis by TGF-β1 signaling pathway in Sprague Dawley rats.[Pubmed: 25443416]|
|The study focussed on the cardioprotective effect of Robinin on doxorubicin-induced cardio-toxicity in Sprague Dawley rats. The supplementation of the Robinin along with DOX caused normalised level of all the above parameters and cardio-toxicity. This study revealed the cardioprotective nature of Robinin on doxorubicin-induced cardiac toxicity by modulating TGF-β1 signaling pathway in Sprague Dawley rats.|
J Pharm Biomed Anal. 2011 Apr 28;55(1):109-13.
|Simultaneous determination of the flavonoids robinin and kaempferol in human breast cancer cells by liquid chromatography-tandem mass spectrometry.[Pubmed: 21232900]|
|An accurate, precise and sensitive method was developed and validated for the simultaneous quantification of the flavonoid glycoside Robinin, and its algycone kaempferol in human breast cancer MCF-7 cells. The assay had a limit of detection of 0.1ng/ml for both compounds when present in cell lysate. The calibration curves were linear from 1 to 250ng/ml (r>0.999) for each compound. The intra- and inter-day coefficients of variation were less than 10% and intra- and inter-day accuracies were within 11%. This assay was successfully applied in a Robinin cellular uptake study to determine the intracellular concentrations of Robinin in MCF-7 cells.|