|Source:||The herbs of Trifolium pratense L.|
|Biological Activity or Inhibitors:||1. Sativan exhibits antituberculosis activity against Mycobacterium tuberculosis H37Rv, with the MIC value of 50 ug/mL.
2. Sativan can markedly reduce the expressions of matrix metalloproteinase-1 caused by ultraviolet irradiated cultured primary human skin fibroblasts.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||3.4926 mL||17.463 mL||34.926 mL||69.8519 mL||87.3149 mL|
|5 mM||0.6985 mL||3.4926 mL||6.9852 mL||13.9704 mL||17.463 mL|
|10 mM||0.3493 mL||1.7463 mL||3.4926 mL||6.9852 mL||8.7315 mL|
|50 mM||0.0699 mL||0.3493 mL||0.6985 mL||1.397 mL||1.7463 mL|
|100 mM||0.0349 mL||0.1746 mL||0.3493 mL||0.6985 mL||0.8731 mL|
Pharmaceuticals (Basel). 2012 Aug 23;5(8):882-9.
|The Chemical Components of Sesbania grandiflora Root and Their Antituberculosis Activity.[Pubmed: 24280680 ]|
|Three isoflavanoids, isovestitol (1), medicarpin (2), and Sativan (3), along with another known compound, betulinic acid (4), were isolated from the root of Sesbania grandiflora. The structures of the isolated compounds were characterised by means of spectroscopic techniques (UV, IR, MS, 1H- and 13C-NMR, DEPT, COSY, HMQC, HMBC, and MS analysis). All the tested compounds 1-4 exhibited antituberculosis activity against Mycobacterium tuberculosis H37Rv, with MIC values of 50 μg/mL for compounds 1-3, and 100 μg/mL for compound 4, whereas, the methanol extract exhibited antituberculosis activity of 625 μg/mL. This is the first report on the occurrence of isoflavonoids in this plant and their antituberculosis activity.|
J Ethnopharmacol. 2006 Mar 8;104(1-2):12-7.
|The effect of sativan from Viola verecunda A. Gray on the expressions of matrix metalloproteinase-1 caused by ultraviolet irradiated cultured primary human skin fibroblasts.[Pubmed: 16207520]|
|We investigated the effect of MMP-1 inhibitory of the solvent fractions of the same plants (Viola hondoensis, Viola ibukiana and Viola verecunda). Therefore, a strong inhibition was found in the ethylacetate fractions of Viola verecunda with inhibitory activity (>90%) at a concentration of 10 microg/ml. Here we investigated the effect of Sativan isolated from the ethylacetate fractions of Viola verecunda on the expression of MMPs in UV-irradiated human skin fibroblasts in vitro. Sativan markedly reduced UV-induced MMP-1 expression at the protein levels in a dose-dependent manner. Our report is the first description for the ability of Sativan to regulate UV-induced MMP-1 expression.|
Antimicrob Agents Chemother. 1980 Feb;17(2):120-3.
|Susceptibility of zoopathogenic fungi to phytoalexins.[Pubmed: 7387136 ]|
|Phytoalexins are a group of low-molecular-weight antibiotics produced by higher plants in response to infection by relatively avirulent microorganisms. They are of relatively low toxicity for mammalian cells and have been reported to possess a broad spectrum of antimicrobial activity against bacteria and phytopathogenic fungi. Employing a broth dilution technique, we have found the zoopathogens Petriellidium boydii, Aspergillus flavus, A. fumigatus, Candida albicans, Coccidioides immitis, Cryptococcus neoformans, Histoplasma capsulatum, Rhizopus oryzae, Sporothrix schenckii, and Trichophyton rubrum to be inhibited by one or more of the phytoalexins maackiain, medicarpin, phaseollin, phaseollin isoflavan, pisatin, Sativan, and vestitol in concentrations of from 12.5 to 50 microgram/ml. Phaseollin isofalvan was the most effective of these. In agar cup diffusion tests growth of the yeast form of Blastomyces dermatitidis was inhibited by phaseollin at 50 microgram/ml and by phaseollin isoflavan at 25 microgram/ml. Susceptibility of the mycelial form of B. dermatitidis to these two compounds was demonstrated by inhibition of radial extension on agar plates.|