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    Thalidezine
    Thalidezine
    Information
    CAS No. 18251-36-0 Price
    Catalog No.CFN89488Purity>=98%
    Molecular Weight638.74Type of CompoundAlkaloids
    FormulaC38H42N2O7Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison (Web)
    Citing Use of our Products
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    According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
    Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
    Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
    Our products had been exported to the following research institutions and universities, And still growing.
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  • Biological Activity
    Description: Thalidezine, a novel AMPK activator, eliminates apoptosis-resistant cancer cells through energy-mediated autophagic cell death, it can inhibit the growth of mouse L1210 cells in vitro. Thalidezine possesses antimicrobial activity against Mycobacterium smegmatis at concentrations of 100 microgram/ml or less.
    Targets: AMPK | Autophagy | Antifection
    In vitro:
    Yao Xue Xue Bao. 1990;25(5):330-5.
    Anti-tumor effect of hernandezine and other components extracted from Thalictrum glandulosissimum。[Pubmed: 2284950]

    METHODS AND RESULTS:
    The total alkaloids of T. glandulosissimum and its main component hernandezine were found to be effective for treatment of mice bearing P388 leukemia, S180 ascites and C26 colon cancer. Although hernandezine inhibited the growth of mouse L1210 cells and human oral cancer KB cells in vitro markedly, its inhibitory effect on normal hemopoietic progenitor cells (CFU-GM) in mice was relatively low.
    CONCLUSIONS:
    Preliminary results showed that hernandezine blocked cell-cycle transfer from G1 to S phase, and its cytocidal action might be cell cycle specific. In addition, two other components, Thalidezine and isoThalidezine, isolated from the same plant exerted similar inhibitory effect on L1210 cells.
    Lloydia. 1977 Jul-Aug;40(4):384-94.
    Alkaloids of Thalictrum. XXI. Isolation and characterization of alkaloids from the roots of Thalictrum podocarpum.[Pubmed: 331006]

    METHODS AND RESULTS:
    Thirteen alkaloids, hernandezine, Thalidezine, N-desmethylThalidezine, isoThalidezine, thalistyline, thalistyline methodiiodide, N-desemethylthalistyline, berberine, columbamine, jatrorrhizine, palmatine, thalifendine, magnoflorine and the artifact, 8-trichloromethyldihydroberberine were isolated from the roots of Thalictrum podocarpum Humb. In addition, oxyberberine and thaliglucinone were obtained in very minor amounts and identified by tlc.
    CONCLUSIONS:
    Of these compounds, N-desmethylThalidezine and isoThalidezine are new bisbenzylisoquinoline alkaloids. Sucrose was isolated from the alcoholic extract. Hernandezine, thalistyline, Thalidezine, thalistyline methodiiodide and N-desmethylthalistyline were found to possess antimicrobial activity against Mycobacterium smegmatis at concentrations of 100 microgram/ml or less.
    Thalidezine Description
    Source: The roots of Thalictrum podocarpum Humb.
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.
    IF=36.216(2019)

    PMID: 29328914

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    IF=22.415(2019)

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    PMID: 30417089
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.5656 mL 7.8279 mL 15.6558 mL 31.3116 mL 39.1396 mL
    5 mM 0.3131 mL 1.5656 mL 3.1312 mL 6.2623 mL 7.8279 mL
    10 mM 0.1566 mL 0.7828 mL 1.5656 mL 3.1312 mL 3.914 mL
    50 mM 0.0313 mL 0.1566 mL 0.3131 mL 0.6262 mL 0.7828 mL
    100 mM 0.0157 mL 0.0783 mL 0.1566 mL 0.3131 mL 0.3914 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Kinase Assay:
    Oncotarget. 2017 May 2;8(18):30077-30091.
    Thalidezine, a novel AMPK activator, eliminates apoptosis-resistant cancer cells through energy-mediated autophagic cell death.[Pubmed: 28404910]
    Cancers illustrating resistance towards apoptosis is one of the main factors causing clinical failure of conventional chemotherapy. Innovative therapeutic methods which can overcome the non-apoptotic phenotype are needed. The AMP-activated protein kinase (AMPK) is the central regulator of cellular energy homeostasis, metabolism, and autophagy. Our previous study showed that the identified natural AMPK activator is able to overcome apoptosis-resistant cancer via autophagic cell death. Therefore, AMPK is an ideal pharmaceutical target for chemoresistant cancers.
    METHODS AND RESULTS:
    Here, we unravelled that the bisbenzylisoquinoline alkaloid Thalidezine is a novel direct AMPK activator by using biolayer interferometry analysis and AMPK kinase assays. The quantification of autophagic EGFP-LC3 puncta demonstrated that Thalidezine increased autophagic flux in HeLa cancer cells. In addition, metabolic stress assay confirmed that Thalidezine altered the energy status of our cellular model. Remarkably, Thalidezine-induced autophagic cell death in HeLa or apoptosis-resistant DLD-1 BAX-BAK DKO cancer cells was abolished by addition of autophagy inhibitor (3-MA) and AMPK inhibitor (compound C). The mechanistic role of autophagic cell death in resistant cancer cells was further supported through the genetic removal of autophagic gene7 (Atg7).
    CONCLUSIONS:
    Overall, Thalidezine is a novel AMPK activator which has great potential to be further developed into a safe and effective intervention for apoptosis- or multidrug-resistant cancers.