ChemFaces is a professional high-purity natural products manufacturer.
Product Intended Use
1. Reference standards
2. Pharmacological research
3. Inhibitors
Citing Use of our Products
How to Order
Orders via your E-mail:
1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Sent to Email: info@chemfaces.com
Contact Us
Order & Inquiry & Tech Support
Tel: (0086)-27-84237683
Fax: (0086)-27-84254680
E-mail: manager@chemfaces.com
Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC
Delivery time
Delivery & Payment method
1. Usually delivery time: Next day delivery by 9:00 a.m. Order now
2. We accept: Wire transfer & Credit card & Paypal & Western Union
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock |
10 mM * 1 mL in DMSO / $206.7 / In-stock |
Other Packaging |
*Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap |
More articles cited ChemFaces products.
Natural Product Communications...2020...Phytomedicine.2022, 100:154058.Journal of Plant Growth Regulatio...2022...J of Essential Oil Research...2019...Int J Mol Sci.2019, 20(23):E6071Int J Mol Sci.2021, 22(11):5503.Int J Pharmacol2020, 16:1-9Life Sci.2021, 286:120019.
ARPN Journal of Eng.& Applied Sci...2016...Heliyon2020, 6(6):e04337.Molecules.2016, 21(6)Free Radic Biol Med.2016, 97:307-319Phytomedicine.2017, 24:77-86Molecules.2019, 24(4):E744Molecules.2016, 21(6)J of the Korean Society of Food S...2016...
Molecules.2018, 23(2)The Japan Society for Analy. Chem...2017...Int J Mol Sci.2022, 23(15):8687. Drug Dev Res.2022, 83(7):1673-1682. BMC Microbiol.2019, 19(1):78Life Sci.2022, 298:120488.Saf Health Work.2019, 10(2):196-204
More...
Our products had been exported to the following research institutions and universities, And still growing.
Indian Institute of Science (India)University of Bonn (Germany)Chinese University of Hong Kong (China)Colorado State University (USA)
University of Brasilia (Brazil)Institute of Tropical Disease U... (Indonesia)Complutense University of Madrid (Spain)National Hellenic Research Foun... (Greece)
University of Eastern Finland (Finland)Yale University (USA)University of Hawaii Cancer Center (USA)
More...
25S-Inokosterone
Inquire / Order:
manager@chemfaces.com
Technical Inquiries:
service@chemfaces.com
Tel:
+86-27-84237783
Fax:
+86-27-84254680
Address:
1 Building, No. 83, CheCheng Rd., Wuhan Economic and Technological Development Zone, Wuhan, Hubei 430056, PRC
Bioorg Med Chem.2018, 26(14):4201-4208Int J Mol Sci.2021, 22(10):5181.J of the Korean Society of Food Science and Nutrition2016, 45(7):1017-1025J Chem Inf Model.2021, 61(11):5708-5718. Front Chem.2022, 10:1048467.FEBS Lett.2015, 589(1):182-7Neurotox Res.2020, 38(1):163-174.Int J Mol Sci.2022, 23(10):5813. Biomedicines.2022, 10(5):1170J Inflamm Res.2022, 15:5347-5359.
Related Screening Libraries
Size /Price /Stock |
10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
|
Related Libraries |
|
Description: |
25S-Inokosterone and 25R-inokosterone exhibit potent inhibition (80-95% at ) against TNF-expression levels in A23187 plus phorbol-myrisrate acetate-induced RBL-2H3 cells, they have excellent anti-atopy activity, thus they could be used to a large range of functional anti-atopy cosmetics. |
Targets: |
TNF-α | IFN-γ |
In vitro: |
Journal of Applied Biological Chemistry, 2015 , 58 (1) :13-19. | Phytoecdysones from the Roots of Achyranthes japonica Nakai and their Anti-atopy Activity[Reference: WebLink] | The roots of Achyranthes japonica Nakai were extracted with 100% aqueous and concentrated subfraction was separated with ultra-performance liquid chromatography-based activity profiling.
METHODS AND RESULTS:
Three compounds were isolated from the subfraction 5 through the repeated prep- high performance liquid chromatography column chromatography. According to the results of physico-chemical and spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as ecdysterone (1), 25S-Inokosterone (2), and 25R-inokosterone (3). Three phytoecdysones were showed weak inhibitory activity for thymus and activation-regulated chemokine expression levels in tumor necrosis factor (TNF)-α plus IFN-γ induced HaCaT cells, respectively. However, those compounds 1-3 were exhibited the most potent inhibition (80–95% at 200 μg/mL) against TNF-α expression levels in A23187 plus phorbol-myrisrate acetate-induced RBL-2H3 cells.
CONCLUSIONS:
As result, 100% aqueous extract of A. japonica has an excellent anti-atopy activity. It could be used to a large range of functional anti-atopy cosmetics. |
|
In vivo: |
Arch Pharm Res . 2012 Aug;35(8):1449-55. | High performance liquid chromatography used for quality control of Achyranthis Radix[Pubmed: 22941488] | Abstract
To establish a standard of quality control and to identify reliable Achyranthis Radix, three phytoecdysones including ecdysterone (1), 25R-inokosterone (2) and 25S-Inokosterone (3) were determined by quantitative HPLC/UV analysis. Three phytoecdysones were separated with an YMC J'sphere ODS C(18) column (250 mm × 4.6 mm, 4 μm) by isocratic elution using 0.1% formic acid in water and acetonitrile (85:15, v/v%) as the mobile phase. The flow rate was 1.0 mL/min and the UV detector wavelength was set at 245 nm. The standards were quantified by HPLC/UV from Achyranthes bidentata Blume and Achyranthes japonica Nakai, as well as Cyathula capitata Moq. and Cyathula officinalis Kuan, which are of a different genus but are comparative herbs. The method was successfully used in the analysis of Achyranthis Radix of different geographical origin or genera with relatively simple conditions and procedures, and the assay results were satisfactory for linearity, recovery, precision, accuracy, stability and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of eighteen A. bidentata Blume samples and ten A. japonica Nakai samples. The results indicate that the established HPLC/UV method is suitable for quantitation and pattern recognition analyses for quality evaluation of Achyranthis Radix. |
|
25S-Inokosterone Description
Source: |
The roots of Achyranthes bidentata Blume. |
Solvent: |
Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc. |
Storage: |
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
|
After receiving: |
The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling. |
ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.
IF=13.297(2019)PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
|
1 mg |
5 mg |
10 mg |
20 mg |
25 mg |
1 mM |
2.0807 mL |
10.4037 mL |
20.8073 mL |
41.6146 mL |
52.0183 mL |
5 mM |
0.4161 mL |
2.0807 mL |
4.1615 mL |
8.3229 mL |
10.4037 mL |
10 mM |
0.2081 mL |
1.0404 mL |
2.0807 mL |
4.1615 mL |
5.2018 mL |
50 mM |
0.0416 mL |
0.2081 mL |
0.4161 mL |
0.8323 mL |
1.0404 mL |
100 mM |
0.0208 mL |
0.104 mL |
0.2081 mL |
0.4161 mL |
0.5202 mL |
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Structure Identification: |
Arch Pharm Res. 2012 Aug;35(8):1449-55. | High performance liquid chromatography used for quality control of Achyranthis Radix.[Pubmed: 22941488 ] | METHODS AND RESULTS: To establish a standard of quality control and to identify reliable Achyranthis Radix, three phytoecdysones including ecdysterone (1), 25R-inokosterone (2) and 25S-Inokosterone (3) were determined by quantitative HPLC/UV analysis. Three phytoecdysones were separated with an YMC J'sphere ODS C(18) column (250 mm × 4.6 mm, 4 μm) by isocratic elution using 0.1% formic acid in water and acetonitrile (85:15, v/v%) as the mobile phase. The flow rate was 1.0 mL/min and the UV detector wavelength was set at 245 nm. The standards were quantified by HPLC/UV from Achyranthes bidentata Blume and Achyranthes japonica Nakai, as well as Cyathula capitata Moq. and Cyathula officinalis Kuan, which are of a different genus but are comparative herbs. The method was successfully used in the analysis of Achyranthis Radix of different geographical origin or genera with relatively simple conditions and procedures, and the assay results were satisfactory for linearity, recovery, precision, accuracy, stability and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of eighteen A. bidentata Blume samples and ten A. japonica Nakai samples.
CONCLUSIONS:
The results indicate that the established HPLC/UV method is suitable for quantitation and pattern recognition analyses for quality evaluation of Achyranthis Radix. |
|
Tags: buy 25S-Inokosterone | 25S-Inokosterone supplier | purchase 25S-Inokosterone | 25S-Inokosterone cost | 25S-Inokosterone manufacturer | order 25S-Inokosterone | 25S-Inokosterone distributor