3-O-Acetyl-16 alpha-hydroxydehydrotrametenolic acid | CAS:168293-14-9

3-O-Acetyl-16 alpha-hydroxydehydrotrametenolic acid

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Product Name	3-O-Acetyl-16 alpha-hydroxydehydrotrametenolic acid
Price:	$238 / 5mg
CAS No.:	168293-14-9
Catalog No.:	CFN90791
Molecular Formula:	C₃₂H₄₈O₅
Molecular Weight:	512.7 g/mol
Purity:	>=98%
Type of Compound:	Triterpenoids
Physical Desc.:	Powder
Source:	The sclerotium of Poria cocos(Schw.) Wolf
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download:	COA MSDS SDF
Similar structural:	Comparison (Web) (SDF)
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Size /Price /Stock	10 mM * 1 mL in DMSO / $181.7 / In-stock
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Description:

3-O-Acetyl-16 alpha-hydroxydehydrotrametenolic acid shows anti-inflammatory activity, it can inhibit NO production and iNOS expression in LPS-stimulated Raw264.7 cells.

Targets:

NO | NOS | PGE | COX

In vitro:

Bioorg Chem. 2017 Feb;70:94-99.

Bioactivity-guided isolation of anti-inflammatory triterpenoids from the sclerotia of Poria cocos using LPS-stimulated Raw264.7 cells.[Pubmed: 27912907 ]

Poria cocos Wolf (Polyporaceae) has been used as a medicinal fungus to treat various diseases since ancient times. This study aimed to investigate the anti-inflammatory chemical constituents of the sclerotia of P. cocos.
METHODS AND RESULTS:
Based on bioassay-guided fractionation using lipopolysaccharide (LPS)-stimulated Raw264.7 cells, chemical investigation of the EtOH extract of the sclerotia of P. cocos resulted in the isolation and identification of eight compounds including six triterpenoids, namely poricoic acid A (1), 3-O-Acetyl-16 alpha-hydroxydehydrotrametenolic acid(2), polyporenic acid C (3), 3β-hydroxylanosta-7,9(11),24-trien-21-oic acid (4), trametenolic acid (5), and dehydroeburicoic acid (6), as well as (-)-pinoresinol (7) and protocatechualdehyde (8). The structures of the isolated compounds were determined by spectroscopic analysis, including 1H and 13C NMR spectra, and LC/MS analysis. The anti-inflammatory activities of the isolates were evaluated by estimating their effect on the production of nitric oxide (NO) and prostaglandin E2 (PGE2) in LPS-stimulated Raw264.7 as well as on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Compounds 1-5 inhibited NO production and iNOS expression in LPS-stimulated Raw264.7 cells. Among them, compound 1 exerted the highest anti-inhibitory activity and reduced PGE2 levels via downregulation of COX-2 protein expression.
CONCLUSIONS:
The findings of this study provide experimental evidence that the sclerotia of P. cocos are a potential source of natural anti-inflammatory agents for use in pharmaceuticals and functional foods. Furthermore, the most active compound 1, seco-lanostane triterpenoid, could be a promising lead compound for the development of novel anti-inflammatory agents.

3-O-Acetyl-16 alpha-hydroxydehydrotrametenolic acid Description

Source:	The sclerotium of Poria cocos(Schw.) Wolf
Solvent:	Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage:	Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C). Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving:	The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.

Structure Identification:

J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Nov 1;1004:10-6. doi: 10.1016/j.jchromb.2015.09.017. Epub 2015 Sep 26.

One-step separation of nine structural analogues from Poria cocos (Schw.) Wolf. via tandem high-speed counter-current chromatography.[Pubmed: 26435185]

A novel one-step separation strategy-tandem high-speed counter-current chromatography (HSCCC) was developed with a six-port valve serving as the switch interface.
METHODS AND RESULTS:
Nine structural analogues including three isomers were successfully isolated from Poria cocos (Schw.) Wolf. by one step. Compared with conventional HSCCC, peak resolution of target compounds was effectively improved in tandem one. Purities of isolated compounds were all over 90% as determined by HPLC. Their structures were then identified via UV, MS and (1)H NMR, and eventually assigned as poricoic acid B (1), poricoic acid A (2), 3β,16α-dihydroxylanosta-7, 9(11), 24-trien-21-oic acid (3), dehydrotumulosic acid (4), polyporenic acid C (5), 3-epi-dehydrotumulosic acid (6), 3-O-Acetyl-16 alpha-hydroxydehydrotrametenolic acid (7), dehydropachymic acid (8) and dehydrotrametenolic acid (9) respectively.
CONCLUSIONS:
The results indicated that tandem HSCCC can effectively improve peak resolution of target compounds, and can be a good candidate for HSCCC separation of structural analogues.

Size /Price /Stock	10 mM * 100 uL in DMSO / Inquiry / In-stock 10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries	Anti-inflammatory Compound Library Triterpenoids Compound Library PGE Inhibitor Library NOS Inhibitor Library NO Inhibitor Library COX Inhibitor Library

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	1.9505 mL	9.7523 mL	19.5046 mL	39.0092 mL	48.7615 mL
5 mM	0.3901 mL	1.9505 mL	3.9009 mL	7.8018 mL	9.7523 mL
10 mM	0.195 mL	0.9752 mL	1.9505 mL	3.9009 mL	4.8761 mL
50 mM	0.039 mL	0.195 mL	0.3901 mL	0.7802 mL	0.9752 mL
100 mM	0.0195 mL	0.0975 mL	0.195 mL	0.3901 mL	0.4876 mL

CFN92825	Parkeol	514-45-4	426.7	C₃₀H₅₀O
CFN92378	Cucurbitadienol	35012-08-9	426.7	C₃₀H₅₀O
CFN92367	Inotodiol	35963-37-2	442.7	C₃₀H₅₀O₂
CFN92365	3beta-Hydroxylanosta-8,24-diene-21-al	96574-03-7	440.7	C₃₀H₄₈O₂
CFN92366	Trametenolic acid	24160-36-9	456.7	C₃₀H₄₈O₃
CFN90990	Tsugaric acid A	174391-64-1	498.8	C₃₂H₅₀O₄
CFN90991	Beta-Elemonic acid	28282-25-9	454.7	C₃₀H₄₆O₃
CFN90803	16 alpha-Hydroxytrametenolic acid	176390-68-4	472.7	C₃₀H₄₈O₄
CFN90804	3-O-Acetyl-16 alpha-hydroxytrametenolic acid	168293-13-8	514.8	C₃₂H₅₀O₅
CFN90577	Dehydrotrametenolic acid	29220-16-4	454.69	C₃₀H₄₆O₃