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Asiaticoside B
Asiaticoside B
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Asiaticoside B
Price: $158 / 20mg
CAS No.: 125265-68-1
Catalog No.: CFN95124
Molecular Formula: C48H78O20
Molecular Weight: 975.13 g/mol
Purity: >=98%
Type of Compound: Triterpenoids
Physical Desc.: Powder
Source: The herbs of Centella asiatica (L.) Urban
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Download: COA    MSDS
Similar structural: Comparison (Web)  (SDF)
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According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $76.7 / In-stock
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Product Use Citation
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Asiaticoside B has notable cytotoxicity against HepG2 and MCF-7 cancer cell lines.
In vitro:
J Nat Prod. 2006 Oct;69(10):1500-2.
Cytotoxic cycloartane triterpene saponins from Actaea asiatica.[Pubmed: 17067171 ]

METHODS AND RESULTS:
Three new 9,19-cycloartane triterpene glycosides, asiaticoside A (1), Asiaticoside B (2), and 25-O-ethylcimigenol-3-O-beta-D-xylopyranoside (3), together with cimiacemoside I (4), 25-O-acetylcimigenol-3-beta-O-D-xyloside (5), and 25-anhydrocimigenol-beta-O-D-xyloside (6) were isolated from the roots/rhizomes extract of Actaea asiatica, and their structures were established by spectroscopic methods (IR, HRESIMS, and NMR).
CONCLUSIONS:
Compounds 1-3, 5, and 6 had notable cytotoxicity against HepG2 and MCF-7 cancer cell lines.
Asiaticoside B Description
Source: The herbs of Centella asiatica (L.) Urban
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
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Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.0255 mL 5.1275 mL 10.255 mL 20.5101 mL 25.6376 mL
5 mM 0.2051 mL 1.0255 mL 2.051 mL 4.102 mL 5.1275 mL
10 mM 0.1026 mL 0.5128 mL 1.0255 mL 2.051 mL 2.5638 mL
50 mM 0.0205 mL 0.1026 mL 0.2051 mL 0.4102 mL 0.5128 mL
100 mM 0.0103 mL 0.0513 mL 0.1026 mL 0.2051 mL 0.2564 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
Pharmazie. 2003 Jun;58(6):381-4.
An improved HPLC method for quantitative determination of six triterpenes in Centella asiatica extracts and commercial products.[Pubmed: 12856998]

METHODS AND RESULTS:
An improved HPLC qualitative and quantitative method of six triterpenes (asiaticoside, madecassoside, asiatic acid, madecassic acid, terminolic acid, and Asiaticoside B) in Centella asiatica (raw plant material and preparations) is described in this paper. After 50 minutes the six active triterpenes were separated and detected in the methanolic extract at a limit of 0.01 microg/ml.
CONCLUSIONS:
The method uses a Phenomenex Aqua 5mu C18 (200 A) column as the stationary phase, a gradient mobile phase of water (0.1% TFA), acetonitrile (0.1% TFA), and methyl tert-butyl ether (0.1% TFA), and UV detection at 206 nm. The correlation coefficients for the calibration curves and the recovery rates ranged from 0.995 to 0.999 and from 98.39% to 100.02%, respectively. The qualitative and quantitative results are discussed.
CFN968303-O-Coumaroylasiatic acid143773-52-8634.85C39H54O7
CFN99912Asiaticoside16830-15-2959.12C48H78O19
CFN99913Madecassoside34540-22-2975.13C48H78O20
CFN95124Asiaticoside B125265-68-1975.13C48H78O20
CFN99914Madecassic acid18449-41-7504.70C30H48O6
CFN994812,24-Dihydroxyursolic acid143839-02-5488.7 C30H48O5
CFN96162Barbinervic acid64199-78-6488.7C30H48O5
CFN96828Myrianthic acid89786-84-5504.70C30H48O6
CFN9107219 alpha-Hydroxyasiatic acid70868-78-9504.7C30H48O6
CFN89232Myrianthic acid 3,23-acetonide578710-52-8544.77C33H52O6
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