Science | Nature | Cell | View More
Natural Products
Hypophyllanthin
Hypophyllanthin
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Hypophyllanthin
Price: $238 / 5mg
CAS No.: 33676-00-5
Catalog No.: CFN98431
Molecular Formula: C24H30O7
Molecular Weight: 430.5 g/mol
Purity: >=98%
Type of Compound: Lignans
Physical Desc.: Powder
Source: The herbs of Phyllanthus niruri Linn.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF    Manual
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $238 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • University of Parma (Italy)
  • University of Toulouse (France)
  • Harvard University (USA)
  • Universita' Degli Studi Di Cagl... (Italy)
  • Worcester Polytechnic Institute (USA)
  • Heidelberg University (Germany)
  • University of Hawaii Cancer Center (USA)
  • Universidad de Antioquia (Colombia)
  • Universiti Malaysia Pahang (Malaysia)
  • University of Madras (India)
  • University of Dicle (Turkey)
  • More...
Package
Featured Products
Inotodiol

Catalog No: CFN92367
CAS No: 35963-37-2
Price: $338/5mg
4',5,6,7-Tetramethoxyflavone

Catalog No: CFN91034
CAS No: 1168-42-9
Price: $258/5mg
1,2,3,4,6-O-Pentagalloylglucose

Catalog No: CFN90192
CAS No: 14937-32-7
Price: $80/20mg
Ginsenoside F2

Catalog No: CFN99755
CAS No: 62025-49-4
Price: $40/20mg
Notoginsenoside R4

Catalog No: CFN91142
CAS No: 87741-77-3
Price: $238/5mg
Protopine

Catalog No: CFN99399
CAS No: 130-86-9
Price: $50/20mg
Soyasaponin Ac

Catalog No: CFN90774
CAS No: 133882-74-3
Price: $ / mg
Madecassic acid

Catalog No: CFN99914
CAS No: 18449-41-7
Price: $30/20mg
Cyanidin-3-O-galactoside chloride

Catalog No: CFN92040
CAS No: 27661-36-5
Price: $318/10mg
Lonicerin

Catalog No: CFN95055
CAS No: 25694-72-8
Price: $168/10mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Hypophyllanthin and phyllanthin have antitumour effects against Ehrlich Ascites Carcinoma in mice. Hypophyllanthin can modulate the vascular tension via the endothelium-independent mechanisms, it can directly inhibit P-gp activity and does not interfere with MRP2 activity, it may reversibly inhibit P-gp function. Hypophyllanthin offers a promising means for treatment of chronic muscle pain. Hypophyllanthin also has estrogenic properties against carbofuran induced toxicity in female rats.
Targets: P-gp | Calcium Channel | Potassium Channel | PGE
In vitro:
J Pharm Pharmacol. 2013 Feb;65(2):292-9.
Phyllanthin and hypophyllanthin inhibit function of P-gp but not MRP2 in Caco-2 cells.[Pubmed: 23278697]
The purposes of this study were to investigate the inhibitory effects of two lignans, phyllanthin and Hypophyllanthin, on the function of P-glycoprotein (P-gp) and multidrug resistance protein 2 (MRP2), using the in-vitro model of Caco-2 cells. In addition, the effect of prolonged exposure to these two compounds on the expression of active P-gp was also determined.
METHODS AND RESULTS:
The activity of P-gp and MRP2 was determined in the uptake assays by monitoring the intracellular accumulation of their specific substrates (calcein acetoxymethyl ester and 5(6)-carboxy-2',7'-dichlorofluorescein diacetate, respectively) with fluorescence spectroscopy. Hypophyllanthin and phyllanthin inhibited P-gp function with comparable potencies, but neither compound affected MRP2 activity. When the lignans were washed out before addition of substrate, the inhibitory action of both compounds against P-gp function was lost. These results suggested the reversibility of the inhibition. Moreover, prolonged exposure of the Caco-2 cells to both lignans (up to 7 days) had no effect on P-gp function.
CONCLUSIONS:
Phyllanthin and Hypophyllanthin directly inhibited P-gp activity and did not interfere with MRP2 activity. It was likely that both phyllanthin and Hypophyllanthin could reversibly inhibit P-gp function.
Fitoterapia. 2011 Dec;82(8):1231-6.
Endothelium-independent effects of phyllanthin and hypophyllanthin on vascular tension.[Pubmed: 21893171]
The purpose of this study was to investigate the modulating effects of phyllanthin and Hypophyllanthin on vascular tension, using in the in vitro model of isolated rat aorta.
METHODS AND RESULTS:
Our results indicated that both phyllanthin and Hypophyllanthin significantly relaxed the sustained contraction induced by phenylephrine (PE) in a concentration-dependent manner. In addition, endothelial removal had no significant influence on the vasorelaxation responses of the aortic rings toward these two compounds. Furthermore, both compounds inhibited the contraction of aortic muscle provoked by either PE (1 μM) or KCl (40 mM) as well as the spontaneous contraction of the Ca²⁺-depleted muscle. In high K⁺-Ca²⁺ free solution, phyllanthin (100 μM), but not Hypophyllanthin, significantly inhibited the contractile responses upon cumulative addition of CaCl₂. Both compounds (100 μM) significantly inhibited PE-induced contraction in Ca²⁺-free condition, but could not affect caffeine-induced contraction.
CONCLUSIONS:
Taken together, phyllanthin and Hypophyllanthin could modulate the vascular tension via the endothelium-independent mechanisms. The modulating effects of both compounds were possibly involved with the blockade of Ca²⁺ entry into vascular smooth muscle cells and inhibition of PE-mediated Ca²⁺ release from sarcoplasmic reticulum.
In vivo:
Phytother Res. 2015 Aug;29(8):1202-10.
Pain Modulation by Lignans (Phyllanthin and Hypophyllanthin) and Tannin (Corilagin) Rich Extracts of Phyllanthus amarus in Carrageenan-induced Thermal and Mechanical Chronic Muscle Hyperalgesia.[Pubmed: 25974715 ]
The current study was aimed at evaluating the antihyperalgesic effects of lignans (phyllanthin and Hypophyllanthin) and tannin (corilagin) rich three standardized extracts of Phyllanthus amarus in a model of chronic musculoskeletal inflammatory pain.
METHODS AND RESULTS:
Three percent carrageenan injected in the gastrocnemius muscle produced hyperalgesia to mechanical and heat stimuli ipsilaterally, which spreads to the contralateral side within 7 to 9 days. To investigate the effects on chronic thermal and mechanical hypersensitivity, three extracts of P. amarus in three doses (100, 200, and 400 mg/kg) were administered to animals intraperitoneally from 14th day to 22nd day after intramuscular injection of carrageenan. It was observed that intraperitoneal administrations of Phyllanthus extracts showed antihyperalgesic activity, as they elevated thermal and mechanical threshold, which was supported by histopathological observations along with reduction in prostaglandin E2 (PGE2) concentration.
CONCLUSIONS:
In conclusion, we strongly suggest that the observed antihyperalgesic and antiinflammatory effects of P. amarus in current pain model are mediated via spinal or supraspinal neuronal mechanisms, mainly by inhibition of PGE2. Modulation of chronic muscular inflammation may be due to presence of phytoconstituents like phyllanthin, Hypophyllanthin, and corilagin, which offers a promising means for treatment of chronic muscle pain.
Hypophyllanthin Description
Source: The herbs of Phyllanthus niruri Linn.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
Hainanic acid B

Catalog No: CFN95526
CAS No: 1637737-46-2
Price: $413/5mg
[(1(10)E,2R,4R)]-2-Methoxy-8,12-ep...

Catalog No: CFN95198
CAS No: 75412-95-2
Price: $318/10mg
1-Methyl-2,8-dihydroxy-3-carboxy-9...

Catalog No: CFN95101
CAS No: 1401414-53-6
Price: $338/5mg
1,7-Diphenyl-5-hydroxy-4,6-hepten-...

Catalog No: CFN95171
CAS No: 87095-77-0
Price: $318/10mg
Methyl lucidenate G

Catalog No: CFN95058
CAS No: 102607-20-5
Price: $413/5mg
Fuzitine

Catalog No: CFN95337
CAS No: 142287-96-5
Price: $318/5mg
Theaflavanoside II

Catalog No: CFN95380
CAS No: 943785-09-9
Price: $318/5mg
4-O-Coumaroylquinic acid

Catalog No: CFN95508
CAS No: 53539-37-0
Price: $318/5mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.3229 mL 11.6144 mL 23.2288 mL 46.4576 mL 58.072 mL
5 mM 0.4646 mL 2.3229 mL 4.6458 mL 9.2915 mL 11.6144 mL
10 mM 0.2323 mL 1.1614 mL 2.3229 mL 4.6458 mL 5.8072 mL
50 mM 0.0465 mL 0.2323 mL 0.4646 mL 0.9292 mL 1.1614 mL
100 mM 0.0232 mL 0.1161 mL 0.2323 mL 0.4646 mL 0.5807 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Animal Research:
Pharmacologyonline, 2008, 2:796-807.
Antitumour effect of Phyllanthin and Hypophyllanthin from Phyllanthus amarus against Ehrlich Ascites Carcinoma in mice.[Reference: WebLink]

METHODS AND RESULTS:
A mixture (1:1) of Phyllanthin and Hypophyllanthin isolated from Phyllanthus amarus (P. amarus) exhibited antitumor activities against Ehrlich Ascites Carcinoma in Swiss albino mice. Animals were pre-treated orally with the extract at a dose of 25mg/kg, 50 mg/kg, 100mg/kg body weight and then after 24hrs after EAC (at a dose of 2 × 10 6 cells/ mouse) administration and following an 18hr fasting; mice were sacrificed for studying of antitumor activity. The decrement of tumor volume, and packed cell volume and viable cell count were observed in lignans treated mice when compared only to EAC tumor bearing mice. Treatment with test compounds increased the survival time and normal peritoneal cell count. Hematological parameters, PCV which were altered by tumor volume inoculation, were restored considerably.
CONCLUSIONS:
Thus, this study was an attempt to evaluate the preventive and curative role of P.amarus lignans in tumor bearing mice.
Structure Identification:
J Nat Prod. 1993 Feb;56(2):233-9.
1H- and 13C-nmr assignments of phyllanthin and hypophyllanthin: lignans that enhance cytotoxic responses with cultured multidrug-resistant cells.[Pubmed: 8385184]

METHODS AND RESULTS:
Complete 1H-nmr data and unambiguous assignments of the 13C-nmr spectra of phyllanthin [1] and Hypophyllanthin [2] were obtained through extensive nmr studies, including homonuclear COSY, homonuclear decoupling, APT, HETCOR, nOe difference, selective INEPT, and COLOC experiments. The absolute configuration of Hypophyllanthin [2] was determined by cd.
CONCLUSIONS:
Neither of these lignans demonstrated significant cytotoxic activity when evaluated with a battery of cultured mammalian cells, but both were found to enhance the cytotoxic response mediated by vinblastine with multidrug-resistant KB cells. In addition, 1 was found to displace the binding of vinblastine with membrane vesicles derived from this cell line, suggesting an interaction with the P-glycoprotein.
Inotodiol

Catalog No: CFN92367
CAS No: 35963-37-2
Price: $338/5mg
4',5,6,7-Tetramethoxyflavone

Catalog No: CFN91034
CAS No: 1168-42-9
Price: $258/5mg
1,2,3,4,6-O-Pentagalloylglucose

Catalog No: CFN90192
CAS No: 14937-32-7
Price: $80/20mg
Ginsenoside F2

Catalog No: CFN99755
CAS No: 62025-49-4
Price: $40/20mg
Notoginsenoside R4

Catalog No: CFN91142
CAS No: 87741-77-3
Price: $238/5mg
Protopine

Catalog No: CFN99399
CAS No: 130-86-9
Price: $50/20mg
Soyasaponin Ac

Catalog No: CFN90774
CAS No: 133882-74-3
Price: $ / mg
Madecassic acid

Catalog No: CFN99914
CAS No: 18449-41-7
Price: $30/20mg
Cyanidin-3-O-galactoside chloride

Catalog No: CFN92040
CAS No: 27661-36-5
Price: $318/10mg
Lonicerin

Catalog No: CFN95055
CAS No: 25694-72-8
Price: $168/10mg
Tags: buy Hypophyllanthin | Hypophyllanthin supplier | purchase Hypophyllanthin | Hypophyllanthin cost | Hypophyllanthin manufacturer | order Hypophyllanthin | Hypophyllanthin distributor