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    Natural Products
    Isorhamnetin-3-O-neohespeidoside
    Information
    CAS No. 55033-90-4 Price $118 / 20mg
    Catalog No.CFN99744Purity>=98%
    Molecular Weight624.54Type of CompoundFlavonoids
    FormulaC28H32O16Physical DescriptionYellow powder
    Download Manual    COA    MSDS    SDFSimilar structuralComparison (Web)  (SDF)
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    * Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
    According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
    Size /Price /Stock 10 mM * 1 mL in DMSO / $60.6 / In-stock
    Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
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    Isorhamnetin-3-O-neohespeidoside

    Isorhamnetin-3-O-neohespeidoside
    Product Name Isorhamnetin-3-O-neohespeidoside
    CAS No.: 55033-90-4
    Catalog No.: CFN99744
    Molecular Formula: C28H32O16
    Molecular Weight: 624.54 g/mol
    Purity: >=98%
    Type of Compound: Flavonoids
    Physical Desc.: Yellow powder
    Source: The barks of Rhamnus purshiana
    Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
    Price: $118 / 20mg
    Inquire / Order: manager@chemfaces.com
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    Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
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    Description: Isorhamnetin 3-O-neohesperoside is the major active substance of Puhuang, a traditional herb medicine widely used in clinical practice to tackle many chronic diseases. It has significant biological and pharmacological activities, including antioxidant, antiatherogenic and antimicrobial effects.
    Isorhamnetin-3-O-neohespeidoside Description
    Source: The barks of Rhamnus purshiana
    Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.6012 mL 8.0059 mL 16.0118 mL 32.0236 mL 40.0295 mL
    5 mM 0.3202 mL 1.6012 mL 3.2024 mL 6.4047 mL 8.0059 mL
    10 mM 0.1601 mL 0.8006 mL 1.6012 mL 3.2024 mL 4.0029 mL
    50 mM 0.032 mL 0.1601 mL 0.3202 mL 0.6405 mL 0.8006 mL
    100 mM 0.016 mL 0.0801 mL 0.1601 mL 0.3202 mL 0.4003 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Structure Identification:
    J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Jan 1;974:131-7.
    Measurement of hydroxysafflor yellow A in human urine by liquid chromatography-tandem mass spectrometry.[Pubmed: 25463208]

    METHODS AND RESULTS:
    A rapid and specific high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the quantification of hydroxysafflor yellow A (HSYA) in human urine with Isorhamnetin-3-O-neohespeidoside as internal standard (IS). HSYA and IS were extracted from urine samples by simple solid-phase extraction and separated on an Agilent Zorbax SB C18 column (4.6 mm × 150 mm, 5 μm) with the mobile phase of 0.2 mM ammonium acetate: methanol (30/70, v/v) at a flow rate of 0.4 mL/min. Polar endogenous interferences eluted in 0.1-2.5 min were switched into waste channel by the Valve Valco, to reduce the possible matrix effect for MS detection in each run. The MS detection of analytes was performed on a tandem mass spectrometer equipped with an electrospray ionization source in negative mode using multiple-reaction monitoring. The MS/MS ion transitions monitored were m/z 611.3→491.2 for HSYA and m/z 623.2→299.2 for IS. The method was fully validated for selectivity, sensitivity, linearity, precision, accuracy, recovery, matrix effect and stability, and then was applied to the urinary excretion study of injectable powder of pure HSYA in healthy Chinese volunteers for the first time.
    CONCLUSIONS:
    The results suggested that urine was the main excretion way of HSYA in healthy volunteers, further demonstrating the feasibility and necessity of our current method.
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