|Zygote. 2014 Feb;22(1):50-7. |
|Effect of lanosterol on the in vitro maturation in semi-defined culture system of prepubertal ewe oocytes.[Pubmed: 21838962]|
|The choice of medium and supplements can affect meiotic regulation and may have an impact on the regulation of mammalian oocyte growth and embryonic cell function. The aim of the present study was to assess the effects of oxygen concentration and endogenous Lanosterol on the in vitro maturation (IVM) media without serum and based on recombinant human chorionic gonadotrophin in prepubertal ewe oocytes. |
METHODS AND RESULTS:
Firstly, the effect of varying oxygen concentrations (5% and 20%) during IVM in TCM-199 supplemented (4 mg/ml bovine serum albumin (BSA), 100 μM cysteamine, 0.3 mM sodium pyruvate, 0.1 UI/ml recombinant follicle-stimulating hormone (r-FSH; Gonal-F® 75 UI, Serono, Italy), 0.1 UI/ml recombinant leuteinizing hormone (r-LH; Lhadi® 75 UI, Serono, Italy) and 1 μg/ml estradiol-17β) on subsequent nuclear maturation of oocytes examined under ultraviolet light following staining with bisbenzimide (Hoechst 33342) was investigated. Secondly, two concentrations of Lanosterol (0, 10 and 50 μM) were added to the IVM medium. Nuclear maturation of oocytes was examined as previously. Lipid content in oocytes, an important indicator of cytoplasmic maturity, was also measured using Nile red fluorescent stain. The results showed that low oxygen concentration affected the nuclear maturation. Similarly, a significantly higher rate of meiosis resumption was observed with 10 μM (72.3%) of Lanosterol compared with the control (51.8%) or 50 μM of Lanosterol (59.4%). A significantly higher content of lipids was also observed with 10 and 50 μM of Lanosterol (7.3 ± 0.2 × 10(6) and 7.4 ± 0.2 × 10(6) arbitrary units of fluorescence) compared with the control (6.7 ± 0.2 × 10(6) arbitrary units of fluorescence).
The results indicate that 10 μM Lanosterol during IVM in medium without serum and based on recombinant human chorionic gonadotrophin has a positive effect on maturation of prepubertal ewe oocytes.
|Cell Death Differ. 2012 Mar;19(3):416-27. |
|Lanosterol induces mitochondrial uncoupling and protects dopaminergic neurons from cell death in a model for Parkinson's disease.[Pubmed: 21818119]|
|To investigate the anti-HBV constituents in the roots of Euphorbia fischeriana.|
METHODS AND RESULTS:
The compounds were isolated by various chromatographic methods and identified by spectroscopic analysis. Some compounds were tested for the anti-HBV activity. Eleven compounds were isolated and identified as tirucalla-5,24-dien-3-ol (1), 24-methyltirucalla-5, 24-dien-3-ol (2), euphol (3), Butyrospermol (4), 24-methylenecycloartenol (5), cycloartenol (6), jolkinolid E (7) helioscopinolide A (8), isoscopoletion (9), dephnoretin (10), and 3, 3'-di-O-methylellagic acid 4'-O-beta-D-xylopyranoside (11).
Compounds 1, 2 and 10 were isolated from the genus Euphorbia for the first time. Compounds 3, 4 and 11 were isolated from this species for the first time. Compounds 1, 8, 9 and 11 showed weak anti-HBsAg and anti-HBeAg activity, while compound 10 showed weak anti-HBsAg activity.