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    Natural Products
    Macrophylloside D
    Macrophylloside D
    Information
    CAS No. 179457-69-3 Price $318 / 10mg
    Catalog No.CFN95113Purity>=98%
    Molecular Weight558.5Type of CompoundPhenols
    FormulaC25H34O14Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison
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    According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
    Size /Price /Stock 10 mM * 1 mL in DMSO / $229.0 / In-stock
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    Macrophylloside D

    Macrophylloside D
    Product Name Macrophylloside D
    CAS No.: 179457-69-3
    Catalog No.: CFN95113
    Molecular Formula: C25H34O14
    Molecular Weight: 558.5 g/mol
    Purity: >=98%
    Type of Compound: Phenols
    Physical Desc.: Powder
    Source: The herbs of Gentiana macrophylla
    Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
    Price: $318 / 10mg
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    Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
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    Description: Reference standards.
    Macrophylloside D Description
    Source: The herbs of Gentiana macrophylla
    Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.7905 mL 8.9526 mL 17.9051 mL 35.8102 mL 44.7628 mL
    5 mM 0.3581 mL 1.7905 mL 3.581 mL 7.162 mL 8.9526 mL
    10 mM 0.1791 mL 0.8953 mL 1.7905 mL 3.581 mL 4.4763 mL
    50 mM 0.0358 mL 0.1791 mL 0.3581 mL 0.7162 mL 0.8953 mL
    100 mM 0.0179 mL 0.0895 mL 0.1791 mL 0.3581 mL 0.4476 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Structure Identification:
    J Sep Sci. 2016 Dec;39(24):4723-4731.
    Efficient methods for isolating five phytochemicals from Gentiana macrophylla using high-performance countercurrent chromatography.[Pubmed: 27774729 ]

    METHODS AND RESULTS:
    Efficient high-performance countercurrent chromatography methods were developed to isolate five typical compounds from the extracts of Gentiana macrophylla. n-Butanol-soluble extract of G. macrophylla contained three hydrophilic iridoids, loganic acid (1), swertiamarin (2) and gentiopicroside (3), and a chromene derivative, Macrophylloside D (4) which were successfully isolated by flow rate gradient (1.5 mL/min in 0-60 min, 5.0 mL/min in 60-120 min), and consecutive flow rate gradient HPCCC using n-butanol/0.1% aqueous trifluoroacetic acid (1:1, v/v, normal phase mode) system. The yields of 1-4 were 22, 16, 122, and 6 mg, respectively, with purities over 97% in a flow rate gradient high-performance countercurrent chromatography, and consecutive flow rate gradient high-performance countercurrent chromatography gave 1, 2, 3 (54, 41, 348 mg, respectively, purities over 97%) and 4 (13 mg, purity at 95%) from 750 mg of sample. The main compound in methylene chloride soluble extract, 2-methoxyanofinic acid, was successfully separated by n-hexane/ethyl acetate/methanol/water (4:6:4:6, v/v/v/v, flow-rate: 4 mL/min, reversed phase mode) condition.
    CONCLUSIONS:
    The structures of five isolates were elucidated by 1 H, 13 C NMR and ESI-Q-TOF-MS spectroscopic data which were compared with previously reported values.
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