Science | Nature | Cell | View More
Natural Products
Methyl protogracillin
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Methyl protogracillin
Price: $318 / 10mg
CAS No.: 54522-53-1
Catalog No.: CFN80084
Molecular Formula: C52H86O23
Molecular Weight: 1078.55 g/mol
Purity: >=98%
Type of Compound: Steroids
Physical Desc.: Powder
Source: The roots of Dioscorea opposita Thunb.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Download: COA    MSDS
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $341.9 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • University of South Australia (Australia)
  • University of Queensland (Australia)
  • University of Wuerzburg (Germany)
  • Seoul National University of Sc... (Korea)
  • Monash University Sunway Campus (Malaysia)
  • CSIRO - Agriculture Flagship (Australia)
  • National Chung Hsing University (Taiwan)
  • University of Hawaii Cancer Center (USA)
  • University of Parma (Italy)
  • Srinakharinwirot University (Thailand)
  • Utah State University (USA)
  • More...
Package
Featured Products
Demethylsuberosin

Catalog No: CFN96241
CAS No: 21422-04-8
Price: $188/20mg
Croceic acid

Catalog No: CFN90226
CAS No: 27876-94-4
Price: $60/20mg
Tannic acid

Catalog No: CFN90501
CAS No: 1401-55-4
Price: $40/20mg
Tectoridin

Catalog No: CFN99921
CAS No: 611-40-5
Price: $50/20mg
Physalin A

Catalog No: CFN92940
CAS No: 23027-91-0
Price: $318/5 mg
(E)-6-O-(p-coumaroyl)scandoside me...

Catalog No: CFN95192
CAS No: 83946-90-1
Price: $218/10mg
Sweroside

Catalog No: CFN99455
CAS No: 14215-86-2
Price: $40/20mg
Alloisoimperatorin

Catalog No: CFN95012
CAS No: 35214-83-6
Price: $338/5mg
2-Hydroxynaringenin

Catalog No: CFN97950
CAS No: 58124-18-8
Price: $ / mg
Acetylshikonin

Catalog No: CFN90523
CAS No: 24502-78-1
Price: $138/20mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Methyl protoneogracillin shows cytotoxic activitiy against the cancer cell line of K562 in vitro as antineoplastic agents. It can cause morphological abnormality of Pyricularia oryzae mycelia.
Targets: Antifection
In vitro:
Phytother Res. 2003 Jun;17(6):620-6.
The cytotoxicity of methyl protoneogracillin (NSC-698793) and gracillin (NSC-698787), two steroidal saponins from the rhizomes of Dioscorea collettii var. hypoglauca, against human cancer cells in vitro.[Pubmed: 12820229]
In our continuous studies of anticancer activity of steroidal saponins from the rhizomes of Dioscorea collettii var. hypoglauca (Dioscoreaceae), methyl protoneogracillin (NSC-698793) and gracillin (NSC-698787) were tested for cytotoxicity against human cancer cell lines from leukemia and eight solid tumor diseases.
METHODS AND RESULTS:
As a result, methyl protoneogracillin was cytotoxic against all the test cell lines with GI(50) < 100 micro M, especially selectively against two leukemia lines (CCRF-CEM and RPMT-8226), one colon cancer line (KM12), two central nervous system (CNS) cancer lines (SF-539 and U251), one melanoma line (M14), one renal cancer line (786-0), one prostate cancer line (DU-145), and one breast cancer line (MDA-MB-435), with GI(50) < or = 2.0 micro M. Leukemia, CNS cancer, and prostate cancer were the most sensitive subpanels, while ovarian cancer was the least sensitive subpanels. The preliminary toxicity studies showed that the maximum tolerant dose was 600 mg/kg for methyl protoneogracillin to mice. Gracillin was cytotoxic against most cell lines with GI(50), TGI and LC(50) at micromolar levels, but no activity against EKVX (non-small cell lung cancer), HT29 (colon cancer), OVCAR-5 (ovarian cancer), and SN12C (renal cancer). Based on structure-activity relationship, C-25 R/S con fi guration was critical for leukemia selectivity between methyl protoneogracillin and Methyl protogracillin. F-ring was critical to selectivity between furostanol (methyl protoneogracillin and Methyl protogracillin) and spirostanol (gracillin) saponins in this study.
CONCLUSIONS:
By an analysis of COMPARE software, no compounds in the NCI's database had similar mean graphs to those of methyl protoneogracillin and gracillin, respectively, indicating potential novel mechanism(s) of action involved. Put all in together, methyl protoneogracillin has been selected as a potential anticancer candidate for hollow fi ber assay to nude mice, but gracillin will not be pursued due to lack of selectivity against human cancer diseases.
Methyl protogracillin Description
Source: The roots of Dioscorea opposita Thunb.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
Caohuoside E

Catalog No: CFN95016
CAS No: 174286-23-8
Price: $318/5mg
Euphorbia factor L25

Catalog No: CFN95339
CAS No: 303174-98-3
Price: $368/5mg
Dipsacus saponin X

Catalog No: CFN95359
CAS No: 146100-01-8
Price: $318/20mg
3'-Hydroxy-5,7,4',5'-Tetramethoxyf...

Catalog No: CFN95397
CAS No: 29976-51-0
Price: $318/5mg
19-O-beta-D-carboxyglucopyranosyl-...

Catalog No: CFN95217
CAS No: 1011714-20-7
Price: $318/5mg
Kihadanin D

Catalog No: CFN95482
CAS No: 2770024-83-2
Price: $413/5mg
Diosmin Impurity 5

Catalog No: CFN95311
CAS No: 122087-66-5
Price: $318/10mg
3',4',5',5,7-Pentamethoxyflavanone

Catalog No: CFN95414
CAS No: 479672-30-5
Price: $218/20mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 0.9272 mL 4.6359 mL 9.2717 mL 18.5434 mL 23.1793 mL
5 mM 0.1854 mL 0.9272 mL 1.8543 mL 3.7087 mL 4.6359 mL
10 mM 0.0927 mL 0.4636 mL 0.9272 mL 1.8543 mL 2.3179 mL
50 mM 0.0185 mL 0.0927 mL 0.1854 mL 0.3709 mL 0.4636 mL
100 mM 0.0093 mL 0.0464 mL 0.0927 mL 0.1854 mL 0.2318 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
Planta Med. 1997 Apr;63(2):161-5.
Antineoplastic agents. II. Four furostanol glycosides from rhizomes of Dioscorea collettii var. hypoglauca.[Pubmed: 17252340 ]

METHODS AND RESULTS:
During activity-guided fractionations to screen for antineoplastic agents, further studies by means of preparative HPLC led to the isolation of four known furostanol saponins: protoneodioscin, protodioscin, protoneogracillin, protogracillin, along with their corresponding artifacts: methyl protoneodioscin, methyl protodioscin, methyl protoneogracillin, and Methyl protogracillin, from the rhizomes of Dioscorea collettii var. hypoglauca. Among them, protoneodioscin, protodioscin, and protoneogracillin are first reported from the title plant. The structures of the compounds were established on the basis of chemical evidence and spectral analysis (1H-NMR, 13C-IMMR, 1H-1H COSY, HMQC, HMBC, and FAB-MS).
CONCLUSIONS:
These eight compounds all caused morphological abnormality of Pyricularia oryzae mycelia. They also showed cytotoxic activities against the cancer cell line of K562 in vitro as antineoplastic agents.
Demethylsuberosin

Catalog No: CFN96241
CAS No: 21422-04-8
Price: $188/20mg
Croceic acid

Catalog No: CFN90226
CAS No: 27876-94-4
Price: $60/20mg
Tannic acid

Catalog No: CFN90501
CAS No: 1401-55-4
Price: $40/20mg
Tectoridin

Catalog No: CFN99921
CAS No: 611-40-5
Price: $50/20mg
Physalin A

Catalog No: CFN92940
CAS No: 23027-91-0
Price: $318/5 mg
(E)-6-O-(p-coumaroyl)scandoside me...

Catalog No: CFN95192
CAS No: 83946-90-1
Price: $218/10mg
Sweroside

Catalog No: CFN99455
CAS No: 14215-86-2
Price: $40/20mg
Alloisoimperatorin

Catalog No: CFN95012
CAS No: 35214-83-6
Price: $338/5mg
2-Hydroxynaringenin

Catalog No: CFN97950
CAS No: 58124-18-8
Price: $ / mg
Acetylshikonin

Catalog No: CFN90523
CAS No: 24502-78-1
Price: $138/20mg
Tags: buy Methyl protogracillin | Methyl protogracillin supplier | purchase Methyl protogracillin | Methyl protogracillin cost | Methyl protogracillin manufacturer | order Methyl protogracillin | Methyl protogracillin distributor