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Protopseudohypericin
Protopseudohypericin
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Protopseudohypericin
Price: $318 / 10mg
CAS No.: 54328-09-5
Catalog No.: CFN90677
Molecular Formula: C30H18O9
Molecular Weight: 522.46 g/mol
Purity: >=98%
Type of Compound: Anthraquinones
Physical Desc.: Yellow powder
Source: The herbs of Hypericum perforatum L.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF
Similar structural: Comparison (Web)  (SDF)
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According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $214.6 / In-stock
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
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Biological Activity
Description: Reference standards.
In vitro:
Phytochemical Analysis,2007,18(3): 204–208.
Simultaneous determination of total hypericin and hyperforin in St. John's wort extracts by HPLC with electrochemical detection[Reference: WebLink]

METHODS AND RESULTS:
An analytical procedure was developed for the simultaneous determination of total hypericin (Protopseudohypericin, pseudohypericin, protohypericin and hypericin) and hyperforin in Hypericum perforatum (St. John's wort) extracts and its preparations. The determination of total hypericin and hyperforin in one step was achieved by exposing the samples to artificial daylight in amber glass vials.
CONCLUSIONS:
This procedure allows both the photoconversion of the protoforms into the appropriate hypericins and the protection of the photosensitive hyperforin. For quantification, an HPLC method with electrochemical detection was applied. As an example of the application of the principle, two preparations containing St. John's wort were assayed.
Protopseudohypericin Description
Source: The herbs of Hypericum perforatum L.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.914 mL 9.5701 mL 19.1402 mL 38.2804 mL 47.8506 mL
5 mM 0.3828 mL 1.914 mL 3.828 mL 7.6561 mL 9.5701 mL
10 mM 0.1914 mL 0.957 mL 1.914 mL 3.828 mL 4.7851 mL
50 mM 0.0383 mL 0.1914 mL 0.3828 mL 0.7656 mL 0.957 mL
100 mM 0.0191 mL 0.0957 mL 0.1914 mL 0.3828 mL 0.4785 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
Monatshefte für Chemie, 1992,123( 8-9):731-739.
On the nature of “soluble” hypericin inHypericum species[Reference: WebLink]

METHODS AND RESULTS:
The two red and two violet “soluble” pigments ofHypericum species were isolated by means of extraction, chromatography, and counter-current droplet chromatography. In contrast to authentic hypericin, they are soluble in common organic solvents and even in water. Using NMR experiments it was deduced that hypericin, pseudohypericin, protohypericin, and Protopseudohypericin are present in the plant as their rapidly interconverting 3- and 4-phenolate ions. From AAS the main counter-ion of these phenolates was derived to be potassium. The potassium and N-ethyl-N,N-diisopropylammonium salts of hypericin were synthesizcd for comparison.
CONCLUSIONS:
A preparative procedure to isolate hypericin and pseudohypericin from plant material was developed.
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Prunasin

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Oxoglaucine

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Sesamolin

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Malvidin chloride

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Squalene

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Oleanolic acid

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