|Pancreatology, 2006 ,6 (3) :175-9. |
|Belgian Pancreatic Club Session at the 18th Belgian Week of Gastroenterology[Reference: WebLink]|
METHODS AND RESULTS:
Fractions from the petroleum extracts showed strong antimicrobial activity, against Escherichia coli ATCC 25922, E. coli -LM 1 , E. coli -LM 2 , and Salmonella enteritidis -MI, with MICs values between 31.2 and 125μg/ml. The bioassay-guided fractionation of the petroleum ether extracts led to the isolation of nine terpenes: azorellolide ( 1 ), mulinol ( 2 ), stachytriol ( 3 ),1α,10β,4β,5α-diepoxy-7β-germacran-6β-ol ( 4 ), 1β,10α,4β,5α-diepoxy-7β-germacran-6β-ol ( 5 ), 1,2,3,3α,4,5,6,7,8,8α-decahydro-7-(1-hydroxy-1-methylethyl)-1,4-dimethylazulene-3α,8α-diol ( 6 ), madreporanone ( 7 ), yaretol ( 8 ) and chrysotol or 6β,10β-epoxy-4α-hydroxyguaiane ( 9 ). Compounds 3 , 5 , 6 and 9 are reported here for the first time from A. cryptantha . Their structures and relative configurations have been determinated by means 1D and 2D NMR techniques.
Chrysothol ( 9 ), madreporanone ( 7 ), and stachytriol ( 3 ) showed strong antimicrobial activities (MICs=50–100μg/ml) against enterobacteria E. coli and S. enteritidis . The antibacterial activity found for some of the isolated compounds supports least in part, the commercial exploited of this species to treat food-borne illnesses associated with Gram negative pathogenic bacteria.