|Source:||The fruits of Glycine max (L.) merr.|
|Biological Activity or Inhibitors:||1. Gycitein has weak estrogenic activity.
2. Glycitein inhibits glioma cell invasion through down-regulation of MMP-3 and MMP-9 gene expression.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||3.5179 mL||17.5895 mL||35.1791 mL||70.3581 mL||87.9477 mL|
|5 mM||0.7036 mL||3.5179 mL||7.0358 mL||14.0716 mL||17.5895 mL|
|10 mM||0.3518 mL||1.759 mL||3.5179 mL||7.0358 mL||8.7948 mL|
|50 mM||0.0704 mL||0.3518 mL||0.7036 mL||1.4072 mL||1.759 mL|
|100 mM||0.0352 mL||0.1759 mL||0.3518 mL||0.7036 mL||0.8795 mL|
Food Funct. 2015 Feb 5.
|Isoflavones in food supplements: chemical profile, label accordance and permeability study in Caco-2 cells.[Pubmed: 25653232]|
|In this study, isoflavones (puerarin, daidzin, genistin, daidzein, Glycitein, genistein, formononetin, prunetin, and biochanin A) from food supplements (n = 15) for menopausal symptoms relief are evaluated and compared with the labelled information. Only four supplements complied with the recommendations made by the EC on the tolerable thresholds. The intestinal bioavailability of these compounds was investigated using Caco-2 cells. The apparent permeability coefficients of the selected isoflavonoids across the Caco-2 cells were affected by the isoflavone concentration and product matrix.|
Food Chem. 2012 Dec 1;135(3):1104-11.
|Bioavailability of glycitein relatively to other soy isoflavones in healthy young Caucasian men.[Pubmed: 22953831]|
|Glycitein is one of the best absorbed flavonoids. Plasma steady-state level can be achieved by several intakes a day. Glycitein bioavailability is similar to that of daidzein and its urinary excretion is significantly higher than that of genistein. Equol does not affect Glycitein bioavailability. Knowing Glycitein bioavailability in man is essential for the development of soy-germ-based food supplements for health applications.|
Molecules. 2010 Jul 29;15(8):5153-61.
|Synthesis of beta-maltooligosaccharides of glycitein and daidzein and their anti-oxidant and anti-allergic activities.[Pubmed: 20714292]|
|The production of beta-maltooligosaccharides of Glycitein and daidzein using Lactobacillus delbrueckii and cyclodextrin glucanotransferase (CGTase) as biocatalysts was investigated. The cells of L. delbrueckii glucosylated Glycitein and daidzein to give their corresponding 4'- and 7-O-beta-glucosides. The beta-glucosides of Glycitein and daidzein were converted into the corresponding beta-maltooligosides by CGTase. The 7-O-beta-glucosides of Glycitein and daidzein and 7-O-beta-maltoside of Glycitein showed inhibitory effects on IgE antibody production. On the other hand, beta-glucosides of Glycitein and daidzein exerted 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging activity and supeoxide-radical scavenging activity.|
Chem Biol Interact. 2010 Apr 15;185(1):18-24.
|Glycitein inhibits glioma cell invasion through down-regulation of MMP-3 and MMP-9 gene expression.[Pubmed: 20188714]|
|Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that play a pivotal role in invasion and angiogenesis of malignant glioma cells. Therefore, the inhibition of MMPs has been suggested to be a promising therapeutic strategy for brain tumors. In the present study, we found that Glycitein, a bacterial metabolite of the isoflavone glycitin, inhibits the expression of MMP-3 and MMP-9 at promoter, mRNA, and protein levels in PMA-stimulated U87MG human astroglioma cells. In addition, gelatin zymography showed that Glycitein inhibited the PMA-induced MMP-9 secretion in U87MG cells. A subsequent Matrigel invasion assay revealed that Glycitein suppresses the in vitro invasiveness of glioma cells, which may be at least partly due to the Glycitein-mediated inhibition of MMP-3 and MMP-9. In support of this, treatment of MMP-3- or MMP-9-specific inhibitor significantly suppressed PMA-induced invasion of glioma cells. Further mechanistic studies revealed that Glycitein inhibits the DNA binding and transcriptional activities of NF-kappaB and AP-1, which are important transcription factors for MMP-3 or MMP-9 gene expression. Furthermore, Glycitein suppresses PMA-induced phosphorylation of three types of MAP kinases, which are upstream signaling molecules in MMP gene expressions and NF-kappaB and AP-1 activities in glioma cells. Therefore, the inhibition of MMP-3 and MMP-9 expression by Glycitein may have therapeutic potential for controlling invasiveness of malignant gliomas.|