|Source:||The roots of Salvia miltiorrhiza Bge.|
|Biological Activity or Inhibitors:||1. Hydroxytanshinone IIA in the liver microsome exhibits a sigmoidal kinetics profile.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||3.2222 mL||16.1108 mL||32.2217 mL||64.4434 mL||80.5542 mL|
|5 mM||0.6444 mL||3.2222 mL||6.4443 mL||12.8887 mL||16.1108 mL|
|10 mM||0.3222 mL||1.6111 mL||3.2222 mL||6.4443 mL||8.0554 mL|
|50 mM||0.0644 mL||0.3222 mL||0.6444 mL||1.2889 mL||1.6111 mL|
|100 mM||0.0322 mL||0.1611 mL||0.3222 mL||0.6444 mL||0.8055 mL|
Journal of Chromatography A Volume 1104, Issues 1–2, 3 February 2006, Pages 366–369
|Identification of tanshinone IIA metabolites in rat liver microsomes by liquid chromatography–tandem mass spectrometry[Reference: WebLink]|
|The results showed the formation of three main hydroxyl metabolites. The three hydroxyl metabolites of tanshinone IIA were proved to be tanshinone IIB, Hydroxytanshinone IIA and przewaquinone A by comparing the tandem mass spectra and the chromatographic retention time with that of the respective authentic compounds. Tanshinone IIB, Hydroxytanshinone IIA and przewaquinone A are all the chemical components of total tanshinones. It was reasonable to presume that the three hydroxy metabolites of tanshinone IIA were pharmacologically active the same as tanshinone IIA and the total tanshinones.|
Rapid Commun Mass Spectrom. 2006;20(5):815-22.
|Simultaneous determination of tanshinone IIA and its three hydroxylated metabolites by liquid chromatography/tandem mass spectrometry.[Pubmed: 16470728]|
|A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the simultaneous determination of tanshinone IIA and its three hydroxylated metabolites, tanshinone IIB, Hydroxytanshinone IIA and przewaquinone A, in a rat liver microsome was developed and fully validated.This validated method was successfully applied to the enzyme kinetic study of tanshinone IIA in liver microsome. The elimination of tanshinone IIA and formation of tanshinone IIB and Hydroxytanshinone IIA in the liver microsome all exhibited a sigmoidal kinetics profile. The formation of przewaquinone A shows a typical hyperbolic profile. In addition, this method has now been applied in the analysis of other bio-samples including plasma, urine, bile and feces.|
J Mass Spectrom. 2006 May;41(5):670-84.
|Characterization of metabolites of tanshinone IIA in rats by liquid chromatography/tandem mass spectrometry.[Pubmed: 16598708]|
|The seven phase I metabolites were formed, through two main metabolic routes, which were hydroxylation and dehydrogenation metabolism. M1, M4, M5 and M6 were supposedly tanshinone IIB, Hydroxytanshinone IIA, przewaquinone A and dehydrotanshinone IIA, respectively, by comparing their HPLC retention times and mass spectral patterns with those of the standard compounds.|