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More articles cited ChemFaces products.
Molecules. 2016 Oct 20Journal of Pharmaceutical Analysis2016 May 5; Food Chem. 2017 Apr 15;Int J Oncol. 2016 Oct;49(4)Horticultural Science2016
Phytomedicine15 Dec. 2015,1262–1268BMC Complement Altern Med.2017 Aug 9;Br J Pharmacol.2018 Mar;Lab On a Chip21 Feb 2018;Braz J Med Biol Res. 2016 Jul 4;49(7).
Advance Publication2015 Aug 18Mol Immunol. 2016 Oct;78:121-132.Hum Exp Toxicol. 2016 Dec 9.Anticancer Res.2014 Jul;34(7):3505-9.
Our products had been exported to the following research institutions and universities, And still growing.
University of Zurich (Switzerland)Kazusa DNA Research Institute (Japan)Guangzhou Institutes of Biomedic... (China)Universidade do Porto (Portugal)
Washington State University (USA)University of Illinois at Chicago (USA)MTT Agrifood Research Finland (Finland)VIT University (India)
University of Limpopo (South Africa)Gyeongsang National University (Korea)University of Madras (India)
||Loureirin A has an inhibitory effect on platelet activation, perhaps through an impairment of PI3K/Akt signaling. Loureirin A activates Wnt/-catenin pathway and promotes hair follicle stem cells (FSCs)-seeded tissue-engineered skin to repair skin wound.The molecular mechanism of Loureirin A and Wnt signaling pathway mediated anti- hepatic fibrosis and anti- angiogenesis may involve down- regulation the expression of Frizzled- 4,inhibiting the synthesis and secretion of α- SMA,TGF- β1and the proliferation of HSCs. |
||Akt | PI3K | c-Myc | GSK-3 | Wnt/β-catenin | TGF-β/Smad | Frizzled- 4|
|Eur J Pharmacol. 2015 Jan 5;746:63-9. |
|Antiplatelet activity of loureirin A by attenuating Akt phosphorylation: In vitro studies.[Pubmed: 25445049]|
|Loureirin A is a flavonoid extracted from Dragon׳s Blood that has been used to promote blood circulation and remove stasis in Chinese traditional medicine. However, the mechanisms of these effects are not fully understood. |
METHODS AND RESULTS:
We explored the anti-platelet activity and underlying mechanism of Loureirin A in vitro. Our results indicated that Loureirin A negatively affected agonist-induced platelet aggregation such as collagen, collagen-related peptide (CRP), ADP and thrombin. Loureirin A inhibited collagen-induced platelet ATP secretion and thrombin-stimulated P-selectin expression in a dose-dependent manner. Platelet spreading on immobilized fibrinogen was significantly impaired in the presence of Loureirin A. Immunoblotting analysis indicated that 100μM of Loureirin A almost completely eliminated collagen-induced Akt phosphorylation at Ser473. Interestingly, a submaximal dose (50μM) of Loureirin A had an additive inhibitory effect with the phosphoinositide 3-kinase (PI3K) inhibitor Ly294002 on collage-induced Akt phosphorylation in platelets.
Taken together, Loureirin A had an inhibitory effect on platelet activation, perhaps through an impairment of PI3K/Akt signaling.
|Journal of Kunming Medical University, 2016 , 37 (6) :13-17. |
|Effect of Loureirin A on Proliferation and Frizzled-4Expression of Rat Hepatic Stellate Cells in vitro[Reference: WebLink]|
|To investigate the molecular mechanism of Loureirin A mediated anti- hepatic fibrosis by evaluting its effects on proliferation,secretion of α- smooth muscle actin(α- SMA) and transforming growth factor- beta1(TGF- β1), and expression of rat hepatic stellate cells in vitro. |
METHODS AND RESULTS:
Primary hepatic stellate cells were isolated and cultured from Sprague- Dawley rats. After activating and inducing primary hepatic stellate cells from q HSC to a HSC, the activated hepatic stellate cells model in vitro was established. Then we observed the morphological changes of static hepatic stellate cells and activated hepatic stellate cells with inverted phase contrast microscope. Cultured hepatic stellate cells were treated with different concentrations of Loureirin A and the inhibitory rate of HSCs proliferation was measured by MTT assay. The expression of Frizzled- 4 was measured by western blot analysis. The content of α- SMA and TGF- β1 in the cultured HSCs' supernatant were measured by enzyme- linked immunosorbent assay(ELISA). Loureirin A the proliferation of inhibited activated hepatic stellate cells in a time- dose- dependent manner compared with the control group,IC50=0.30 μg/μL. After Loureirin A treatment of the HSCs, western blot analysis showed that Frizzled- 4 expression level was obviously lower than control group.Loureirin A also inhibited α- SMA and TGFβ1(P0.05) secretion in the cultured HSCs' supernatant in different degree by the assay of ELISA.
The molecular mechanism of Loureirin A and Wnt signaling pathway mediated anti- hepatic fibrosis and anti- angiogenesis may involve down- regulation the expression of Frizzled- 4,inhibiting the synthesis and secretion of α- SMA,TGF- β1and the proliferation of HSCs.
Loureirin A Description
||The herbs of Dracaena cochinchinensis
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Recent ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.PMID: 29328914
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.PMID: 29149595
Scientific Reports 2017 Dec 11;7(1):17332.doi: 10.1038/s41598-017-17427-6.PMID: 29230013
Molecules. 2017 Oct 27;22(11). pii: E1829.doi: 10.3390/molecules22111829.PMID: 29077044
J Cell Biochem. 2018 Feb;119(2):2231-2239.doi: 10.1002/jcb.26385. PMID: 28857247
Phytomedicine. 2018 Feb 1;40:37-47. doi: 10.1016/j.phymed.2017.12.030.PMID: 29496173
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
|Journal of Biomaterials & Tissue Engineering, 2016 , 6 (6) :427-432. |
|Loureirin A Activates Wnt/β-Catenin Pathway to Promote Wound with Follicle Stem Cell-Seeded Tissue-Engineered Skin Healing[Reference: WebLink]|
|Hair follicle stem cells (FSCs) can participate in the formation of hair follicles and epidermis. FSCs are well known as the ideal seed cells for tissue-engineered skin. Loureirin A, the major constituent of resina draconis, can promote skin wound healing. |
METHODS AND RESULTS:
In this study, FSCs and hair follicle fibroblasts were combined with Pelnac® scaffold to construct tissue-engineered skin. The tissue-engineered skin was grafted into full-thickness skin defects of BALB/c-nu mice. Loureirin A was incorporated into the transplanted tissue-engineered skin. On day 3 of post-transplantation, Loureirin A could inhibit inflammatory reaction. On day 7 of post-transplantation, Loureirin A promoted the fibroblasts proliferation to repair dermis, and the newly formed epidermis was thicker than normal epidermis. On day 14 of post-transplantation, Loureirin A promoted the newly formed skin tending to the normal skin. All of these results showed that Loureirin A could promote skin wound repair. Wnt/β-catenin pathway controls natural hair follicle regeneration and epidermis' repairing on skin injuries. In order to validate whether Wnt/β-catenin pathway can be activated by Loureirin A, it was used to stimulate FSCs in vitro. The results displayed that Loureirin A could promote FSCs proliferation and change the cell cycle. Western blot showed that GSK-3β was down-regulated and β-catenin was up-regulated in FSCs by Loureirin A. Simultaneously, downstream genes of Wnt/β-catenin pathway, c-Myc, cyclin D1, Tcf3 and Lef1, also increased.
Overall, these results demonstrate that Loureirin A activates Wnt/β-catenin pathway and promotes FSC-seeded tissue-engineered skin to repair skin wound.
|Eur J Med Chem. 2015 Mar 26;93:492-500. |
|Comparison between loureirin A and cochinchinenin C on the interaction with human serum albumin.[Pubmed: 25734332]|
METHODS AND RESULTS:
The interactions of Loureirin A (LA) and cochinchinenin C (CC) with human serum albumin (HSA) under simulated physiological conditions (pH = 7.4) have been studied with fluorescence, UV-vis absorption spectroscopic method and molecular docking technique. The results indicated that there was a synergistic interaction between LA and CC, and the fluorescence quenching of HSA by LA (or CC) was a combined quenching procedure (dynamic and static quenching). At low compound concentrations, the quenching constants KSV of CC was larger than that of LA, which meant the CC efficacy may be better than that of LA. The negative △H and △S values suggested hydrogen bonds and van der Waals forces played the major role in the binding of LA (or CC) to HSA. The efficiency of energy transfer and distance between the compounds and HSA was calculated.
Moreover, the results of synchronous and three-dimensional fluorescence demonstrated that the HSA microenvironment was changed in the presence of LA (or CC). Finally, the binding of LA (or CC) to HSA was modeled by molecular docking, which is in good accordance with the experimental studies.