|Source:||The roots of Panax notoginseng (Burk.)F.H.Chen|
|Biological Activity or Inhibitors:||1. Notoginsenoside R2 has neuroprotection against 6-OHDA-induced neurotoxicity, is associated with Notoginsenoside R2-mediated P90RSK and Nrf2 activation through MEK1/2-ERK1/2 pathways.
|Solvent:||DMSO, Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||1.297 mL||6.4851 mL||12.9702 mL||25.9403 mL||32.4254 mL|
|5 mM||0.2594 mL||1.297 mL||2.594 mL||5.1881 mL||6.4851 mL|
|10 mM||0.1297 mL||0.6485 mL||1.297 mL||2.594 mL||3.2425 mL|
|50 mM||0.0259 mL||0.1297 mL||0.2594 mL||0.5188 mL||0.6485 mL|
|100 mM||0.013 mL||0.0649 mL||0.1297 mL||0.2594 mL||0.3243 mL|
Evid Based Complement Alternat Med. 2013;2013:971712.
|P90RSK and Nrf2 Activation via MEK1/2-ERK1/2 Pathways Mediated by Notoginsenoside R2 to Prevent 6-Hydroxydopamine-Induced Apoptotic Death in SH-SY5Y Cells.[Pubmed: 24159358]|
|We found that the preincubation of SH-SY5Y cells for 24 h with 20 μ M Notoginsenoside R2 (NGR2), which is a newly isolated notoginsenoside from Panax notoginseng, showed neuroprotective effects against 6-OHDA-induced oxidative stress and apoptosis. Notoginsenoside R2 incubation successively resulted in the activation of P90RSK, inactivation of BAD, and inhibition of 6-OHDA-induced mitochondrial membrane depolarization, thus preventing the mitochondrial apoptosis pathway. Notoginsenoside R2 incubation also led to the activation of Nrf2 and subsequent activity enhancement of phase II detoxifying enzymes, thus suppressing 6-OHDA-induced oxidative stress, and these effects could be removed by Nrf2 siRNA. These results suggested that the neuroprotection elicited by Notoginsenoside R2 against 6-OHDA-induced neurotoxicity was associated with Notoginsenoside R2-mediated P90RSK and Nrf2 activation through MEK1/2-ERK1/2 pathways.|