|Description:||1. Pellitorine shows strong cytotoxic activities against HL60 and MCT-7 cell lines. |
2. Pellitorine can suppress expression of inducible NO synthase and cyclooxygenase-2.
3. Pellitorine shows antiprotozoal activity against Plasmodium falciparum (IC50 = 3.3 ug/mL).
4. Pellitorine is a potential larvicide with a specific target site and a lead molecule for the control of mosquito populations.
5. Pellitorine shows potent antiplatelet aggregation activity.
|Targets:||NOS | COX | Nrf2 | NADPH-oxidase | HO-1 | ATPase | Antifection|
|Source:||The herbs of Anacyclus pyrethrum|
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||4.4763 mL||22.3814 mL||44.7628 mL||89.5255 mL||111.9069 mL|
|5 mM||0.8953 mL||4.4763 mL||8.9526 mL||17.9051 mL||22.3814 mL|
|10 mM||0.4476 mL||2.2381 mL||4.4763 mL||8.9526 mL||11.1907 mL|
|50 mM||0.0895 mL||0.4476 mL||0.8953 mL||1.7905 mL||2.2381 mL|
|100 mM||0.0448 mL||0.2238 mL||0.4476 mL||0.8953 mL||1.1191 mL|
Phytomedicine. 2014 Dec 15;21(14):1801-7.
|Quantitative transdermal behavior of pellitorine from Anacyclus pyrethrum extract.[Pubmed: 25481393]|
|The plant Anacyclus pyrethrum (AP) consists of several N-alkylamides with Pellitorine as main constituent. AP extracts are known to be biologically active and some products for topical administration containing AP plant extracts are already commercially available with functional cosmeceutical claims. However, no transdermal data for Pellitorine are currently available. Therefore, our general goal was to investigate the local skin pharmacokinetics of the plant N-alkylamide Pellitorine using a Franz diffusion cell set-up. Two different forms were applied on human skin: purified Pellitorine and the AP extract. Our study demonstrated that Pellitorine is able to cross the stratum corneum and the subsequent skin layers. A significantly higher permeability coefficient was observed when the AP extract (Kp=2.3 × 10(-4)cm/h) was administered, compared to purified Pellitorine (Kp=1.1 × 10(-4)cm/h). With the obtained Pellitorine concentrations in the skin layers and the receptor fluid, it is concluded that local and systemic effects can be expected after topical application. Due to these findings and as a regulatory consequence, products containing reasonable concentrations of Pellitorine are recommended to be classified as a medicinal product.|
J Agric Food Chem. 2007 Nov 14;55(23):9436-42.
|Isolation and identification of antiplatelet aggregatory principles from the leaves of Piper lolot.[Pubmed: 17941696 ]|
|The methanolic extract of Piper lolot, having shown potent inhibitory activity on platelet aggregation induced by arachidonic acid (AA) and platelet activating factor (PAF), was subjected to activity-guided isolation to yield twelve new amide alkaloids, piperlotine A-L (1-12), along with twenty-nine known compounds. Their structures were elucidated on the basis of spectroscopic analysis. The isolated compounds were tested for their inhibitory activity on the rabbit platelet aggregation. The compounds piperlotine A (1), piperlotine C (3), piperlotine D (4), piperlotine E (5), 3-phenyl-1-(2,4,6-trihydroxyphenyl)propan-1-one (21), 3-(4-methoxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one (22), 1-trans-cinnamoylpyrrolidine (24), sarmentine (26), Pellitorine (27), methyl 3-phenylpropionate (32), and (10S)-10-hydroxypheophorbide a methyl ester (40) showed potent antiplatelet aggregation activity.|
Phytother Res. 2017 Apr;31(4):663-670.
|Alkaloids from Piper nigrum Exhibit Antiinflammatory Activity via Activating the Nrf2/HO-1 Pathway.[Pubmed: 28185326]|
|Consistent with NO inhibition, treatment of RAW264.7 cells with chabamide (1), Pellitorine (2), and 6,7-dehydrobrachyamide B (7) suppressed expression of inducible NO synthase and cyclooxygenase-2. Chabamide (1), Pellitorine (2), and 6,7-dehydrobrachyamide B (7) induced heme-oxygenase-1 expression at the transcriptional level. In addition, compound 1 induced the nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) and upregulated the expression of Nrf2 target genes, NAD(P)H:quinone oxidoreductase 1 and γ-glutamyl cysteine synthetase catalytic subunit, in a concentration-dependent manner in RAW264.7 cells. These findings suggest that chabamide (1) from P. nigrum exert antiinflammatory effects via the activation of the Nrf2/heme-oxygenase-1 pathway; hence, it might be a promising candidate for the treatment of inflammatory diseases.|
Molecules. 2010 Apr 5;15(4):2398-404.
|Pellitorine, a potential anti-cancer lead compound against HL6 and MCT-7 cell lines and microbial transformation of piperine from Piper Nigrum.[Pubmed: 20428051]|
|Pellitorine (1), which was isolated from the roots of Piper nigrum, showed strong cytotoxic activities against HL60 and MCT-7 cell lines. Microbial transformation of piperine (2) gave a new compound 5-[3,4-(methylenedioxy)phenyl]-pent-2-ene piperidine (3). Two other alkaloids were also found from Piper nigrum. They are (E)-1-[3',4'-(methylenedioxy)cinnamoyl]piperidine (4) and 2,4-tetradecadienoic acid isobutyl amide (5). These compounds were isolated using chromatographic methods and their structures were elucidated using MS, IR and NMR techniques.|
Molecules. 2014 May 20;19(5):6428-38.
|Antiprotozoal activity of Achillea ptarmica (Asteraceae) and its main alkamide constituents.[Pubmed: 24853616]|
|All alkamides were tested for antiprotozoal activity in vitro. Pellitorine was the most active compound so far within this study against P. falciparum (IC50 = 3.3 μg/mL), while 8,9-Z-dehydroPellitorine was most active against T. b. rhodesiense (IC50 = 2.0 μg/mL). The activity of pure Pellitorine against Plasmodium is higher than that of the crude extract and thus explains the activity of the latter. None of the isolated alkamides, however, was as active against T. b. rhodesiense as the crude extract whose antitrypanosomal activity must therfore be due to a synergistic effect of the isolated compounds or to more active yet to be identified constituents.|
PLoS One. 2013 Nov 18;8(11):e80226.
|Novel histopathological and molecular effects of natural compound pellitorine on larval midgut epithelium and anal gills of Aedes aegypti.[Pubmed: 24260359]|
|The yellow fever mosquito, Aedes aegypti, is a vector for transmitting dengue fever and yellow fever. In this study, we assessed the histopathological and molecular effects of Pellitorine, an isobutylamide alkaloid, on the third instar of Ae. aegypti larvae. At 5 mg/l concentration of Pellitorine, the whole body of the treated larvae became dark in color, particularly damaged thorax and abdominal regions. Pellitorine was targeted mainly on midgut epithelium and anal gills, indicating variably dramatic degenerative responses of the midgut through a sequential epithelial disorganization. The anterior and posterior midgut was entirely necrosed, bearing only gut lumen residues inside the peritrophic membranes. Pellitorine caused comprehensive damage of anal gill cells and branches of tracheole and debris was found in hemolymph of the anal gills. RT-PCR analysis indicates that the compound inhibited gene expression encoding V-type H(+)-ATPase and aquaporine 4 after treatment with 2.21 mg/l Pellitorine. These results verify that Pellitorine merits further study as a potential larvicide with a specific target site and a lead molecule for the control of mosquito populations.|