ChemFaces is a professional high-purity natural products manufacturer.
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2. Pharmacological research
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According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
|Size /Price /Stock
||10 mM * 1 mL in DMSO / Inquiry||Other Packaging
||*Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
More articles cited ChemFaces products.
Antiviral Res.2013, 98(3):386-93Scientific World Journal....2014...Acta Agriculturae Scandinavica...2015...Acta Physiologiae Plantarum2016, 38:7Food and Bioprocess Technology...2017...Biochem Systematics and Ecology...2017...PLoS One.2017, 12(8):e0181191J Ethnopharmacol.2017, 198:91-97
JPC-Journal of Planar Chromatogra...2017...J.Acta Agriculturae Scandinavica...2017...Korean Journal of Pharmacognosy...2018...Korean J. Crop Sci.2018, 63(2):131-139Front Cell Infect Microbiol.2018, 8:292Pharmacognosy Journal...2019...BMC Complement Altern Med....2019...Int J Mol Sci.2019, 20(8):E1855
J Ethnopharmacol.2019, 228:132-141Molecules.2019, 24(11):E2044J Asian Nat Prod Res.2019, 5:1-17Phytomedicine.2019, 58:152893Phytomedicine.2019, 65:153089Int J Food Sci Nutr.2019, 70(7):825-833J Nat Med.2020, 74(1):65-75
Our products had been exported to the following research institutions and universities, And still growing.
Weizmann Institute of Science (Israel)Shanghai Institute of Organic C... (China)VIB Department of Plant Systems... (Belgium)Chiang Mai University (Thailand)
University of Padjajaran (Indonesia)Technical University of Denmark (Denmark)Almansora University (Egypt)Anna University (India)
Kyushu University (Japan)Max-Planck-Insitut (Germany)Melbourne University (Australia)
Related Screening Libraries
|| Siegeskaurolic acid exhibits anti-inflammatory and antinociceptive effects, which due to
the inhibition of iNOS and COX-2 expression in RAW 264.7 macrophages via NF-kappaB inactivation. |
||NO | PGE | TNF-α | COX | p65 | NOS | NF-kB|
|Eur J Pharmacol. 2007 Mar 8;558(1-3):185-93. |
|Anti-inflammatory activities of ent-16alphaH,17-hydroxy-kauran-19-oic acid isolated from the roots of Siegesbeckia pubescens are due to the inhibition of iNOS and COX-2 expression in RAW 264.7 macrophages via NF-kappaB inactivation.[Pubmed: 17207792]|
|To isolate the anti-inflammatory components in Siegesbeckia pubescens root, we performed activity-guided fractionation using a carrageenan-induced edema rat model. |
METHODS AND RESULTS:
Antinociceptive effects were followed using acetic acid-induced abdominal constriction and hot plate tests in mice. Chloroform extract was subjected to silica gel and octadesyl silane (ODS) column chromatography, and a diterpene was isolated which was identified as ent-16alphaH,17-hydroxy-kauran-19-oic acid (Siegeskaurolic acid). Pretreatment with Siegeskaurolic acid (20 or 30 mg/kg/day, p.o.) exhibited anti-inflammatory and antinociceptive effects in these animal models. To investigate the mechanisms underlying this anti-inflammatory action, we investigated the effect of Siegeskaurolic acid on lipopolysaccharide (LPS)-induced responses in a murine macrophage cell line, RAW 264.7. Siegeskaurolic acid was found to significantly inhibit the productions of nitric oxide (NO), prostaglandin E(2) (PGE(2)), and tumor necrosis factor-alpha (TNF-alpha). Consistent with these findings, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins, and iNOS, COX-2, and TNF-alpha mRNAs were found to be inhibited by Siegeskaurolic acid. Furthermore, Siegeskaurolic acid inhibited the nuclear factor-kappaB (NF-kappaB) activation induced by LPS, and this was associated with the prevention of inhibitor kappaB degradation (I kappaB), and subsequently with decreased nuclear p65 and p50 protein levels.
Taken together, our data indicate that the anti-inflammatory and antinociceptive properties of Siegeskaurolic acid may be due to iNOS, COX-2 and TNF-alpha inhibition via the down-regulation of NF-kappaB binding activity.
Siegeskaurolic acid Description
||The roots of Siegesbeckia pubescens.
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Recent ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)PMID: 29328914
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)PMID: 32004475
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)PMID: 29149595
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)PMID: 29553709
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)PMID: 28005066
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.