| Structure Identification: |
| Planta Medica, 2009, 75(04):s-2009-1216513. | | Quantitative Determination of Cycloartane and Flavonoid Glycosides from Sutherlandia frutescens by UPLC-UV, UPLC-ELSD Methods and Confirmation by UPLC-MS.[Reference: WebLink] |
METHODS AND RESULTS:
Sutherlandia frutescens (L.) R. BR., Family Fabaceae, is a well-known and widely used medicinal plant from the Western Cape, South Africa [1,2]. Traditionally it has been used as a remedy for stomach problems, internal cancers, diabetes and various inflammatory conditions. Recently, it has been used for the management of HIV/AIDS in patients [1]. This paper describes the analytical method suitable for the determination of four flavonoid glycosides (Sutherlandin A, B, C, D) and four cycloartane glycosides (Sutherlandioside A, Sutherlandioside B, Sutherlandioside C, Sutherlandioside D) from stem-leaves of Sutherlandia frutescens (L.) R. BR. A separation by UPLC was achieved by using Acquity shield RP18 column, PDA with ELS detection, and a water/acetonitrile gradient as the mobile phase. The major cycloartane glycoside compound (Sutherlandioside B) was detected at a concentration as low as 1.0 µg/mL. The analysis of plant material and products showed considerable variation of 0.6–2.7% for the major compound. This method involved the use of the [M+H]+ and [M+Na]+ ions in the positive ion mode with extractive ion monitoring (EIM).
CONCLUSIONS:
The eight compounds were further confirmed by UPLC-MS method in plant sample and products. In the positive ion mode, the protonated species [M+H]+ at m/z 741.2, 741.2, 725.2, 725.2, 653.4, 651.4, 635.4 and 653.4 and sodiated species [M+Na]+ at m/z 763.2, 763.2, 747.2, 747.2, 675.4, 673.4, 657.4 and 675.4 for compounds 1–8 were observed.
|
|
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)