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    Natural Products
    Withanolide S
    Withanolide S
    Information
    CAS No. 63139-16-2 Price
    Catalog No.CFN96434Purity>=98%
    Molecular Weight504.61Type of CompoundSteroids
    FormulaC28H40O8Physical DescriptionPowder
    Download     COA    MSDSSimilar structuralComparison (Web)
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    Description: Reference standards.
    Withanolide S Description
    Source: The herbs of Physali alkekengi
    Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.
    IF=36.216(2019)

    PMID: 29328914

    Cell Metab. 2020 Mar 3;31(3):534-548.e5.
    doi: 10.1016/j.cmet.2020.01.002.
    IF=22.415(2019)

    PMID: 32004475

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    IF=14.548(2019)

    PMID: 29149595

    ACS Nano. 2018 Apr 24;12(4): 3385-3396.
    doi: 10.1021/acsnano.7b08969.
    IF=13.903(2019)

    PMID: 29553709

    Nature Plants. 2016 Dec 22;3: 16206.
    doi: 10.1038/nplants.2016.205.
    IF=13.297(2019)

    PMID: 28005066

    Sci Adv. 2018 Oct 24;4(10): eaat6994.
    doi: 10.1126/sciadv.aat6994.
    IF=12.804(2019)

    PMID: 30417089
    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.9817 mL 9.9086 mL 19.8173 mL 39.6346 mL 49.5432 mL
    5 mM 0.3963 mL 1.9817 mL 3.9635 mL 7.9269 mL 9.9086 mL
    10 mM 0.1982 mL 0.9909 mL 1.9817 mL 3.9635 mL 4.9543 mL
    50 mM 0.0396 mL 0.1982 mL 0.3963 mL 0.7927 mL 0.9909 mL
    100 mM 0.0198 mL 0.0991 mL 0.1982 mL 0.3963 mL 0.4954 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Structure Identification:
    Journal of Separation Science, 2017,41(2):518-524.
    Validated thin-layer chromatographic method for the identification and monitoring of the effect of the extraction method on the yield and phytochemical constituents of Egyptian Withania somnifera leaves.[Pubmed: 29115713]
    A sensitive, reliable, simple and rapid thin-layer chromatographic method has been developed for routine analysis of Withanolide S content for the purpose of quality control assessment of chemotype III of Withania somnifera.
    METHODS AND RESULTS:
    The new method was used first to compare the accumulation of Withanolide S in different parts of the plant, which was found to be the highest in the leaves extract (0.21% w/w). Second, to investigate different extraction parameters that improve the extraction efficiency of withanolides from the leaves using conventional and ultrasound-assisted extraction methods.
    CONCLUSIONS:
    The extraction efficiency was expressed via total withanolide content and Withanolide S content.