|Source:||The root of Scutellaria baicalensis Georgi|
|Biological Activity or Inhibitors:||1. Baicalin is a flavonoid and a major component of a herbal medicine, Sho-saiko-to, which is commonly used for treatment of chronic hepatitis in Japan and China, baicalin acts as anti-oxidants, also causes cytotoxic effect,and induces caspase-3 activation and apoptosis via mitochondrial pathway.
2. Baicalin has anti-inflammatory activity, exhibits the greatest inhibition activity against carrageenan-induced rat paw edema.
3. Baicalin has neuroprotection, may be associated with its anti-oxidative and anti-apoptotic properties in global cerebral ischemia in the gerbils.
4. Baicalin may have significant therapeutic benefits against diabetic complications and atherosclerosis.
|Solvent:||Pyridine, Methanol, Ethanol, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.2403 mL||11.2015 mL||22.4029 mL||44.8059 mL||56.0073 mL|
|5 mM||0.4481 mL||2.2403 mL||4.4806 mL||8.9612 mL||11.2015 mL|
|10 mM||0.224 mL||1.1201 mL||2.2403 mL||4.4806 mL||5.6007 mL|
|50 mM||0.0448 mL||0.224 mL||0.4481 mL||0.8961 mL||1.1201 mL|
|100 mM||0.0224 mL||0.112 mL||0.224 mL||0.4481 mL||0.5601 mL|
BMB Rep. 2015 Mar 5. pii: 3111.
|Baicalin, baicalein and wogonin inhibits high glucose-induced vascular inflammation in vitro and in vivo.[Pubmed: 25739393]|
|Data showed that HG induced markedly increased vascular permeability, monocyte adhesion, expressions of cell adhesion molecules (CAMs), formation of reactive oxygen species (ROS) and activation of nuclear factor (NF)-κB. Remarkably, all of the above mentioned vascular inflammatory effects of HG were attenuated by pretreatment with Baicalin, baicalein, and wogonin. Vascular inflammatory responses induced by HG are critical events underlying development of various diabetic complications, therefore, our results suggest that Baicalin, baicalein, and wogonin may have significant therapeutic benefits against diabetic complications and atherosclerosis.|
Inflammation. 2015 Jan 30.
|Baicalin Inhibits Lipopolysaccharide-Induced Inflammation Through Signaling NF-κB Pathway in HBE16 Airway Epithelial Cells.[Pubmed: 25630720]|
|These findings suggest that the anti-inflammatory properties of Baicalin may be resulted from the inhibition of IL-6, IL-8, and TNF-α expression via preventing signaling NF-κB pathway in HBE16 airway epithelial cells. In addition, this study provides evidence to understand the therapeutic effects of Baicalin on inflammatory diseases in clinical practice.|
Mol Immunol. 2002 Feb;38(10):781-91.
|Baicalin induces apoptosis via mitochondrial pathway as prooxidant.[Pubmed: 11841838]|
|Baicalin is a flavonoid and a major component of a herbal medicine, Sho-saiko-to, which is commonly used for treatment of chronic hepatitis in Japan and China. Flavonoids including Baicalin have been reported to not only function as anti-oxidants but also cause cytotoxic effect. We investigated the mechanism of Baicalin-induced cytotoxicity in leukemia-derived T cell line, Jurkat cells. When cells were cultured with 50-200 microg/ml Baicalin for 6h, caspase-3 was activated and then cells fell into apoptosis. Induction of apoptosis by Baicalin was accompanied with the marginal generation of intracellular reactive oxygen species (ROS), the increase of the cytosolic fractions of cytochrome c, and the disruption of mitochondrial transmembrane potential (DeltaPsi(m)) prior to the activation of caspase-3. The pre-culture with 5 mM of buthionine sulfoximine (BSO), an inhibitor of glutathione (GSH) synthesis, facilitated Baicalin-induced disruption of DeltaPsi(m) and induction of apoptosis. The pre-culture with N-benzyloxycarbonyl-valyl-alanyl-aspartyl fluoromethylketone (Z-VAD-fmk), a pan-caspase inhibitor, partially suppressed the induction of apoptosis. On the other hand, Baicalin showed little toxic effect on peripheral blood mononuclear cells (PBMCs) from healthy volunteers. These results indicate that Baicalin acts as a prooxidant and induces caspase-3 activation and apoptosis via mitochondrial pathway.|
Brain Res Bull. 2011 Jul 15;85(6):396-402.
|Baicalin attenuates global cerebral ischemia/reperfusion injury in gerbils via anti-oxidative and anti-apoptotic pathways.[Pubmed: 21600966 ]|
|Histopathological examination showed that the administration of Baicalin by the dose of 100 and 200mg/kg significantly attenuated ischemia-induced neuronal cell damage. Reduced level of MDA, obviously elevated activities of SOD and GSH as well as GSH-PX were also found in Baicalin-treated groups. Further investigation demonstrated that treatment with Baicalin remarkably promoted the expression of BDNF and inhibited the expression of caspase-3 at mRNA and protein levels by real-time RT-PCR and Western blot, respectively. Besides, caspase-3 activity assay also elucidated that the administration of Baicalin could significantly suppress caspase-3 in ischemic gerbils hippocampus. Theses findings suggest that Baicalin's neuroprotection appears to be associated with its anti-oxidative and anti-apoptotic properties in global cerebral ischemia in the gerbils.|