| In vitro: |
| Biological Mass Spectrometry, 2010, 12(4):163-169. | | Identification of urinary 3‐ethoxy‐4‐hydroxybenzoic and 3‐ethoxy‐4‐hydroxymandelic acids after dietary intake of ethyl vanillin.[Reference: WebLink] | The activation of the Agrobacterium virulence system is known to be induced by certain phenolic compounds.
METHODS AND RESULTS:
We have tested the vir-inducing ability of fifty compounds, by using a virB-lacZ gene fusion, and analysed the relationship between structure and activity of these compounds. In this way we have identified several new vir -inducers: coniferylalcohol, 3,5-dimethoxy-4-hydroxybenzene, homovanillic acid, ferulic acid, 3-Ethoxy-4-hydroxybenzaldehyde and guaiacol, all of which are compounds with strong or moderate activity and four compounds with weak vir -inducing activity. In view of the specificity of vir -inducers, our data extended observations of others and enabled us to define the specific structural features of a vir -inducer molecule. In addition we show here that induction of the octopine Ti vir -genes is (i) optimal at 29° C and totally abolished at 37° C., and (ii) strongly inhibited at low concentrations of sodium chloride. The implications for plant transformation are discussed. | | Phytochemical Analysis Pca, 2003,14(3):127-31. | | Specificity of signal molecules in the activation of Agrobacterium virulence gene expression.[Reference: WebLink] | A method has been developed to determine the false-positive effects on acetylcholinesterase inhibition in the TLC assay based on Ellman's method.
METHODS AND RESULTS:
Various aldehydes and amines have been tested in order to determine whether the observed inhibition is due to a true enzyme inhibition or due to the inhibition of the reaction between thiocholine and 5,5′-dithiobis-(2-nitrobenzoic acid). 4-Dimethylaminobenzaldehyde, 3-Ethoxy-4-hydroxybenzaldehyde, diethylamine, triethylamine, triethanolamine and tyramine showed real enzyme inhibition, although their activity was about 10 3 times lower than that shown by galanthamine. Heptanal, decanal, cinnamaldehyde, anisaldehyde, benzaldehyde, hexylamine and tryptamine appeared to show a non-specific chemical inhibition.
CONCLUSIONS:
By checking this chemical inhibition on the TLC assay, the true enzyme inhibition could be distinguished from the false-positive chemical inhibition observed in the toluene extract of Nerine bowdenii in the course of isolation of active compounds. |
|
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)