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3'-Methoxydaidzin
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Product Name 3'-Methoxydaidzin
Price: $218 / 10mg
CAS No.: 200127-80-6
Catalog No.: CFN89443
Molecular Formula: C22H22O10
Molecular Weight: 446.40 g/mol
Purity: >=98%
Type of Compound: Flavonoids
Physical Desc.: Powder
Source: The roots of Pueraria lobata (Willd.) Ohwi.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Download: COA    MSDS
Similar structural: Comparison (Web)  (SDF)
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According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $141.7 / In-stock
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Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
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Biological Activity
Description: 3'-Methoxydaidzin has antioxidation activity, it may be a potential neuroprotective substance in Gegen Qinlian decoction.
In vitro:
Fine Chemicals, 2004, 21(7):525-528.
Antioxidation Activities of Natural Components from Dalbergia odorifera T.Chen.[Reference: WebLink]

METHODS AND RESULTS:
The antioxidation activities of natural components extracted from Dalbergia odorifera T.Chen on lard and ascidian oil were investigated by OSI.Dalbergia odorifera T.Chen was extracted with 95% ethanol,and the extract was then extracted by petroleum ether,CHCl_3,EtOAc and BuOH successively.The residue of EtOAc had very good antioxidation activity.Eight components were isolated from it,i.e.,2,4-dihydroxy-5-methoxybenzophenone(Ⅰ),2′,3′,7-trihydroxy-4-methoxyisoflavone(Ⅱ),3'-Methoxydaidzin(Ⅲ),4′,5,7-trihydroxy-3-methoxyflavone(Ⅳ),vestitol(Ⅴ),medicarpin(Ⅵ),2-propenyl hexanoate(Ⅶ) and ethyl hexadecanoate(Ⅷ).
CONCLUSIONS:
The results showed that components Ⅰ,Ⅲ,Ⅴ and Ⅵ had antioxidation activity and components Ⅱ and Ⅳ had strong antioxidant activities both on lard and ascidian oil at 0.02% and 0.04% levels.
3'-Methoxydaidzin Description
Source: The roots of Pueraria lobata (Willd.) Ohwi.
Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
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IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.2401 mL 11.2007 mL 22.4014 mL 44.8029 mL 56.0036 mL
5 mM 0.448 mL 2.2401 mL 4.4803 mL 8.9606 mL 11.2007 mL
10 mM 0.224 mL 1.1201 mL 2.2401 mL 4.4803 mL 5.6004 mL
50 mM 0.0448 mL 0.224 mL 0.448 mL 0.8961 mL 1.1201 mL
100 mM 0.0224 mL 0.112 mL 0.224 mL 0.448 mL 0.56 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
Zhongguo Zhong Yao Za Zhi. 2013 May;38(10):1581-4.
Development of a rapid screening method for discovering neuroprotective components from traditional Chinese medicine.[Pubmed: 23947142]
This study developed a method for rapid screening neuroprotective compounds with FDA (fluorescein diacetate) labeled SH-SYSY cells, which was injuried by Glu (L-glutamic acid).
METHODS AND RESULTS:
The cell viability was determined by fluorescence automatic mi-croscopy screening system. Then, neuroprotective components from Gegen Qinlian decoction were screened by the proposed method. The results demonstrated that 4 chemical components, C15, D06, D07 and E05 from Gegen Qinlian decoction, showed significantly neuroprotective effects. Furthermore, 8 compounds (i. e. daidzin, 3'-Methoxydaidzin, liquiritin apioside, 6-C-L-alpha-arabinopyranosyl-8-C-beta-D-glucopyranosyl chrysin, isoliquiritin apioside, baicalin, oroxylin-A-7-O-fP-D-glucuronide and wogonoside) were identified from these active components by LC-Q/TOF-MS technology (liquid chromatography quadrupole time of flight tandem mass spectrometry).
CONCLUSIONS:
These 8 compounds may be the potential neuroprotective substances in Gegen Qinlian decoction.
Planta Med. 1998 Oct;64(7):620-7.
Identification of isoflavones in the roots of Pueraria lobata.[Pubmed: 17253303 ]

METHODS AND RESULTS:
The isoflavones of the roots of Pueraria lobata (Willd.) Ohwi (Puerariae Radix) were investigated by high-performance liquid chromatography (HPLC) coupled to photodiode array (PDA) and to mass spectroscopy (MS) using atmospheric pressure chemical ionization (APCI) or electrospray ionization (ESI) in combination with collision-activated decomposition (CAD) (HPLC-APCI-CAD-MS or ESI-CAD-MS) for identification of glycosides and HPLC-APCI-CAD-MS for identification of aglycones. The major glycosides are derived from daidzein ( 9) and most are 8- C-glycosides. 3'-Hydroxypuerarin-4'- O-deoxyhexoside ( 2B) and 3'-methoxy-6''- O- D-xylosylpuerarin ( 6) were identified as new constituents. MS data were obtained for puerarin-4'- O- D-glucoside ( 1), 3'-hydroxypuerarin ( 2A), puerarin ( 3), 3'-methoxypuerarin ( 4), 6''- O- D-xylosylpuerarin ( 5), daidzin ( 7) and 3'-Methoxydaidzin ( 8), which were previously characterized by NMR analysis. Isoflavones identified in Puerariae Radix comprise 3'-methoxydaidzein ( 10), genistein ( 12), daidzein-7- O-methyl ether ( 13A), 3'-methoxydaidzein-7- O-methyl ether or 3'-methoxyformononetin ( 13B) and biochanin A ( 15), while previous characterization of daidzein ( 9) and formononetin ( 14) was substantiated by MS data. The structure of compound 11 could not be established by MS techniques.
CONCLUSIONS:
The estrogenic activity was mainly located in the aglycone fraction.
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