Science | Nature | Cell | View More
Natural Products
Arctigenin
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Arctigenin
Price: $40 / 20mg
CAS No.: 7770-78-7
Catalog No.: CFN99534
Molecular Formula: C21H24O6
Molecular Weight: 372.41 g/mol
Purity: >=98%
Type of Compound: Lignans
Physical Desc.: Powder
Source: The roots of Arctium lappa L.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS    SDF    Manual
Similar structural: Comparison (Web)  (SDF)
Guestbook:
Contact Us
Order & Inquiry & Tech Support

Tel: (0086)-27-84237683
Tech: service@chemfaces.com
Order: manager@chemfaces.com
Address: 176, CheCheng Eest Rd., WETDZ, Wuhan, Hubei 430056, PRC
How to Order
Orders via your E-mail:

1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Order: manager@chemfaces.com
Delivery time
Delivery & Payment method

1. Usually delivery time: Next day delivery by 9:00 a.m. Order now

2. We accept: Wire transfer & Credit card & Paypal
Citing Use of our Products
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / $10.8 / In-stock
Other Packaging *Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
Our products had been exported to the following research institutions and universities, And still growing.
  • Osmania University (India)
  • University of Medicine and Phar... (Romania)
  • The Institute of Cancer Research (United Kingdom)
  • VIB Department of Plant Systems... (Belgium)
  • Sanford Burnham Medical Researc... (USA)
  • University of British Columbia (Canada)
  • University of Zurich (Switzerland)
  • Guangzhou Institutes of Biomedi... (China)
  • Mahidol University (Thailand)
  • University of Indonesia (Indonesia)
  • University of Limpopo (South Africa)
  • More...
Package
Featured Products
Skullcapflavone II

Catalog No: CFN92216
CAS No: 55084-08-7
Price: $218/10mg
Ginsenoside Rc

Catalog No: CFN99973
CAS No: 11021-14-0
Price: $70/20mg
Hesperidin

Catalog No: CFN98839
CAS No: 520-26-3
Price: $30/20mg
Curcumenol

Catalog No: CFN92614
CAS No: 19431-84-6
Price: $70/20mg
Gypenoside XLIX

Catalog No: CFN99717
CAS No: 94987-08-3
Price: $118/20mg
3,3',4',5,6,7,8-heptamethoxyflavone

Catalog No: CFN95021
CAS No: 1178-24-1
Price: $268/10mg
8-Hydroxypinoresinol

Catalog No: CFN92432
CAS No: 81426-17-7
Price: $ / mg
Ganoderic acid X

Catalog No: CFN92996
CAS No: 86377-53-9
Price: $463/5mg
Geraniin

Catalog No: CFN90256
CAS No: 60976-49-0
Price: $100/20mg
Aloin A

Catalog No: CFN99943
CAS No: 1415-73-2
Price: $30/20mg
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Arctigenin has anti-tumor, anti-inflammation, antioxidative , neuroprotection, and endurance enhancement effects; it efficiently enhances rat swimming endurance by elevation of the antioxidant capacity of the skeletal muscles, which has thereby highlighted the potential of this natural product as an antioxidant in the treatment of fatigue and related diseases. Arctigenin as a potent indirect activator of AMPK via inhibition of respiratory complex I, with beneficial effects on metabolic disorders in ob/ob mice, this highlights the potential value of arctigenin as a possible treatment of type 2 diabetes.
Targets: AMPK | PPAR | p53 | Nrf2 | PI3K | Akt | Caspase | mTOR | MAPK | HO-1 | NOS | TNF-α | IL Receptor | IkB | p65 | NF-kB | IKK
In vitro:
Cancer Res. 2006 Feb 1;66(3):1751-7.
Identification of arctigenin as an antitumor agent having the ability to eliminate the tolerance of cancer cells to nutrient starvation.[Pubmed: 16452235]
Tumor cells generally proliferate rapidly and the demand for essential nutrients as well as oxygen always exceeds the supply due to the unregulated growth and the insufficient and inappropriate vascular supply. However, cancer cells show an inherent ability to tolerate extreme conditions, such as that characterized by low nutrient and oxygen supply, by modulating their energy metabolism.
METHODS AND RESULTS:
Thus, targeting nutrient-deprived cancer cells may be a novel strategy in anticancer drug development. Based on that, we established a novel screening method to discover anticancer agents that preferentially inhibit cancer cell viability under the nutrient-deprived condition. After screening 500 medicinal plant extracts used in Japanese Kampo medicine, we found that a CH(2)Cl(2)-soluble extract of Arctium lappa exhibited 100% preferential cytotoxicity under the nutrient-deprived condition at a concentration of 50 microg/mL with virtually no cytotoxicity under nutrient-rich condition. Further bioassay-guided fractionation and isolation led to the isolation of Arctigenin as the primary compound responsible for such preferential cytotoxicity; the compound exhibited 100% preferential cytotoxicity against nutrient-deprived cells at a concentration of 0.01 microg/mL. Furthermore, Arctigenin was also found to strongly suppress the PANC-1 tumor growth in nude mice, as well as the growth of several of the tested pancreatic cancer cell lines, suggesting the feasibility of this novel antiausterity approach in cancer therapy.
CONCLUSIONS:
Further investigation of the mechanism of action of Arctigenin revealed that the compound blocked the activation of Akt induced by glucose starvation, which is a key process in the tolerance exhibited by cancer cells to glucose starvation.
In vivo:
Acta Pharmacol Sin. 2014 Oct;35(10):1274-84.
Arctigenin enhances swimming endurance of sedentary rats partially by regulation of antioxidant pathways.[Pubmed: 25152028]
Arctigenin, a phenylpropanoid dibenzylbutyrolactone lignan found in traditional Chinese herbs, has been determined to exhibit a variety of pharmacological activities, including anti-tumor, anti-inflammation, neuroprotection, and endurance enhancement. In the present study, we investigated the antioxidation and anti-fatigue effects of Arctigenin in rats.
METHODS AND RESULTS:
Rat L6 skeletal muscle cell line was exposed to H2O2 (700 μmol/L), and ROS level was assayed using DCFH-DA as a probe. Male SD rats were injected with Arctigenin (15 mg·kg(-1)·d(-1), ip) for 6 weeks, and then the weight-loaded forced swimming test (WFST) was performed to evaluate their endurance. The levels of antioxidant-related genes in L6 cells and the skeletal muscles of rats were analyzed using real-time RT-PCR and Western blotting. Incubation of L6 cells with Arctigenin (1, 5, 20 μmol/L) dose-dependently decreased the H2O2-induced ROS production. WFST results demonstrated that chronic administration of Arctigenin significantly enhanced the endurance of rats. Furthermore, molecular biology studies on L6 cells and skeletal muscles of the rats showed that Arctigenin effectively increased the expression of the antioxidant-related genes, including superoxide dismutase (SOD), glutathione reductase (Gsr), glutathione peroxidase (GPX1), thioredoxin (Txn) and uncoupling protein 2 (UCP2), through regulation of two potential antioxidant pathways: AMPK/PGC-1α/PPARα in mitochondria and AMPK/p53/Nrf2 in the cell nucleus.
CONCLUSIONS:
Arctigenin efficiently enhances rat swimming endurance by elevation of the antioxidant capacity of the skeletal muscles, which has thereby highlighted the potential of this natural product as an antioxidant in the treatment of fatigue and related diseases.
Int Immunopharmacol. 2014 Dec;23(2):505-15.
Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice.[Pubmed: 25284342]
The crude powder of the fruit of Arctium lappa L. (ALF) has previously been reported to attenuate experimental colitis in mice. But, its main effective ingredient and underlying mechanisms remain to be identified.
METHODS AND RESULTS:
In this study, ALF was extracted with ethanol, and then successively fractionated into petroleum ether, ethyl acetate, n-butanol and water fraction. Experimental colitis was induced by dextran sulfate sodium (DSS) in mice. Among the four fractions of ALF, the ethyl acetate fraction showed the most significant inhibition of DSS-induced colitis in mice. The comparative studies of Arctigenin and arctiin (the two main ingredients of ethyl acetate fraction) indicated that Arctigenin rather than arctiin could reduce the loss of body weight, disease activity index and histological damage in the colon. Arctigenin markedly recovered the loss of intestinal epithelial cells (E-cadherin-positive cells) and decreased the infiltration of neutrophils (MPO-positive cells) and macrophages (CD68-positive cells). Arctigenin could down-regulate the expressions of TNF-α, IL-6, MIP-2, MCP-1, MAdCAM-1, ICAM-1 and VCAM-1 at both protein and mRNA levels in colonic tissues. Also, it markedly decreased the MDA level, but increased SOD activity and the GSH level. Of note, the efficacy of Arctigenin was comparable or even superior to that of the positive control mesalazine. Moreover, it significantly suppressed the phosphorylation of MAPKs and the activation of NF-κB, including phosphorylation of IκBα and p65, p65 translocation and DNA binding activity.
CONCLUSIONS:
In conclusion, Arctigenin but not arctiin is the main active ingredient of ALF for attenuating colitis via down-regulating the activation of MAPK and NF-κB pathways.
Diabetologia. 2012 May;55(5):1469-81.
Arctigenin, a natural compound, activates AMP-activated protein kinase via inhibition of mitochondria complex I and ameliorates metabolic disorders in ob/ob mice.[Pubmed: 22095235 ]
Arctigenin is a natural compound that had never been previously demonstrated to have a glucose-lowering effect. Here it was found to activate AMP-activated protein kinase (AMPK), and the mechanism by which this occurred, as well as the effects on glucose and lipid metabolism were investigated.
METHODS AND RESULTS:
2-Deoxyglucose uptake and AMPK phosphorylation were examined in L6 myotubes and isolated skeletal muscle. Gluconeogenesis and lipid synthesis were evaluated in rat primary hepatocytes. The acute and chronic effects of Arctigenin on metabolic abnormalities were observed in C57BL/6J and ob/ob mice. Changes in mitochondrial membrane potential were measured using the J-aggregate-forming dye, JC-1. Analysis of respiration of L6 myotubes or isolated mitochondria was conducted in a channel oxygen system. Arctigenin increased AMPK phosphorylation and stimulated glucose uptake in L6 myotubes and isolated skeletal muscles. In primary hepatocytes, it decreased gluconeogenesis and lipid synthesis. The enhancement of glucose uptake and suppression of hepatic gluconeogenesis and lipid synthesis by Arctigenin were prevented by blockade of AMPK activation. The respiration of L6 myotubes or isolated mitochondria was inhibited by Arctigenin with a specific effect on respiratory complex I. A single oral dose of Arctigenin reduced gluconeogenesis in C57BL/6J mice. Chronic oral administration of Arctigenin lowered blood glucose and improved lipid metabolism in ob/ob mice.
CONCLUSIONS:
This study demonstrates a new role for Arctigenin as a potent indirect activator of AMPK via inhibition of respiratory complex I, with beneficial effects on metabolic disorders in ob/ob mice. This highlights the potential value of Arctigenin as a possible treatment of type 2 diabetes.
Front Immunol . 2021 Jul 19;12:691590.
Arctigenin Exerts Neuroprotective Effect by Ameliorating Cortical Activities in Experimental Autoimmune Encephalomyelitis In Vivo[Pubmed: 34349758]
Abstract Multiple sclerosis (MS) is a chronic disease in the central nervous system (CNS), characterized by inflammatory cells that invade into the brain and the spinal cord. Among a bulk of different MS models, the most widely used and best understood rodent model is experimental autoimmune encephalomyelitis (EAE). Arctigenin, a botanical extract from Arctium lappa, is reported to exhibit pharmacological properties, including anti-inflammation and neuroprotection. However, the effects of Arctigenin on neural activity attacked by inflammation in MS are still unclear. Here, we use two-photon calcium imaging to observe the activity of somatosensory cortex neurons in awake EAE mice in vivo and found added hyperactive cells, calcium influx, network connectivity, and synchronization, mainly at preclinical stage of EAE model. Besides, more silent cells and decreased calcium influx and reduced network synchronization accompanied by a compensatory rise in functional connectivity are found at the remission stage. Arctigenin treatment not only restricts inordinate individually neural spiking, calcium influx, and network activity at preclinical stage but also restores neuronal activity and communication at remission stage. In addition, we confirm that the frequency of AMPA receptor-mediated spontaneous excitatory postsynaptic current (sEPSC) is also increased at preclinical stage and can be blunted by Arctigenin. These findings suggest that excitotoxicity characterized by calcium influx is involved in EAE at preclinical stage. What is more, Arctigenin exerts neuroprotective effect by limiting hyperactivity at preclinical stage and ameliorates EAE symptoms, indicating that Arctigenin could be a potential therapeutic drug for neuroprotection in MS-related neuropsychological disorders.
Arctigenin Description
Source: The roots of Arctium lappa L.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
alpha-Costic acid

Catalog No: CFN95322
CAS No: 28399-17-9
Price: $318/5mg
Gynosaponin TN2

Catalog No: CFN95331
CAS No: 77658-95-8
Price: $318/5mg
Oleuroside

Catalog No: CFN95099
CAS No: 116383-31-4
Price: $288/20mg
Regaloside I

Catalog No: CFN95525
CAS No: 126239-78-9
Price: $318/5mg
Pinocembroside

Catalog No: CFN95444
CAS No: 75829-43-5
Price: $118/20mg
Senkyunolide N

Catalog No: CFN95449
CAS No: 140694-58-2
Price: $318/10mg
New compound 8

Catalog No: CFN95341
CAS No: N/A
Price: $318/5mg
1-Isomangostin

Catalog No: CFN95114
CAS No: 19275-44-6
Price: $318/5mg
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.6852 mL 13.4261 mL 26.8521 mL 53.7043 mL 67.1303 mL
5 mM 0.537 mL 2.6852 mL 5.3704 mL 10.7409 mL 13.4261 mL
10 mM 0.2685 mL 1.3426 mL 2.6852 mL 5.3704 mL 6.713 mL
50 mM 0.0537 mL 0.2685 mL 0.537 mL 1.0741 mL 1.3426 mL
100 mM 0.0269 mL 0.1343 mL 0.2685 mL 0.537 mL 0.6713 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Kinase Assay:
J Biochem Mol Toxicol. 2015 Apr 28.
Arctigenin, a Natural Lignan Compound, Induces Apoptotic Death of Hepatocellular Carcinoma Cells via Suppression of PI3-K/Akt Signaling.[Pubmed: 25920004]

METHODS AND RESULTS:
In this study, we explored the cytotoxic effects of Arctigenin, a natural lignan compound, on human hepatocellular carcinoma (HCC) cells and check the involvement of phosphatidylinositol 3-kinase (PI3-K)/Akt signaling. HCC cells were treated with different concentrations of Arctigenin and cell viability and apoptosis were assessed. Manipulating Akt signaling was used to determine its role in the action of Arctigenin. Arctigenin significantly inhibited the viability of HCC cells in a concentration-dependent manner. Arctigenin induced apoptosis and activation of caspase-9 and -3. Overexpression of a constitutively active Akt mutant blocked Arctigenin-induced apoptosis. Combinational treatment with Arctigenin and the PI3-K inhibitor LY294002 significantly enhanced apoptosis. Arctigenin reduced the expression of Bcl-xL, Mcl-1, and survivin and the phosphorylation of mTOR and S6K, which were significantly reversed by overexpression of constitutively active Akt.
CONCLUSIONS:
This is the first report about the anticancer activity of Arctigenin in HCC cells, which is mediated by inactivation of PI3-K/Akt signaling.
Animal Research:
Inflammation. 2015 Jan 24.
Arctigenin Protects against Lipopolysaccharide-Induced Pulmonary Oxidative Stress and Inflammation in a Mouse Model via Suppression of MAPK, HO-1, and iNOS Signaling.[Pubmed: 25616905]
Arctigenin, a bioactive component of Arctium lappa (Nubang), has anti-inflammatory activity.
METHODS AND RESULTS:
Here, we investigated the effects of Arctigenin on lipopolysaccharide (LPS)-induced acute lung injury. Mice were divided into four groups: control, LPS, LPS + DMSO, and LPS + Arctigenin. Mice in the LPS + Arctigenin group were injected intraperitoneally with 50 mg/kg of Arctigenin 1 h before an intratracheal administration of LPS (5 mg/kg). Lung tissues and bronchoalveolar lavage fluids (BALFs) were collected. Histological changes of the lung were analyzed by hematoxylin and eosin staining. Arctigenin decreased LPS-induced acute lung inflammation, infiltration of inflammatory cells into BALF, and production of pro-inflammatory cytokines. Moreover, Arctigenin pretreatment reduced the malondialdehyde level and increased superoxide dismutase and catalase activities and glutathione peroxidase/glutathione disulfide ratio in the lung. Mechanically, Arctigenin significantly reduced the production of nitric oxygen and inducible nitric oxygen synthase (iNOS) expression, enhanced the expression of heme oxygenase-1, and decreased the phosphorylation of mitogen-activated protein kinases (MAPKs).
CONCLUSIONS:
Arctigenin has anti-inflammatory and antioxidative effects on LPS-induced acute lung injury, which are associated with modulation of MAPK, HO-1, and iNOS signaling.
Skullcapflavone II

Catalog No: CFN92216
CAS No: 55084-08-7
Price: $218/10mg
Ginsenoside Rc

Catalog No: CFN99973
CAS No: 11021-14-0
Price: $70/20mg
Hesperidin

Catalog No: CFN98839
CAS No: 520-26-3
Price: $30/20mg
Curcumenol

Catalog No: CFN92614
CAS No: 19431-84-6
Price: $70/20mg
Gypenoside XLIX

Catalog No: CFN99717
CAS No: 94987-08-3
Price: $118/20mg
3,3',4',5,6,7,8-heptamethoxyflavone

Catalog No: CFN95021
CAS No: 1178-24-1
Price: $268/10mg
8-Hydroxypinoresinol

Catalog No: CFN92432
CAS No: 81426-17-7
Price: $ / mg
Ganoderic acid X

Catalog No: CFN92996
CAS No: 86377-53-9
Price: $463/5mg
Geraniin

Catalog No: CFN90256
CAS No: 60976-49-0
Price: $100/20mg
Aloin A

Catalog No: CFN99943
CAS No: 1415-73-2
Price: $30/20mg
Tags: buy Arctigenin | Arctigenin supplier | purchase Arctigenin | Arctigenin cost | Arctigenin manufacturer | order Arctigenin | Arctigenin distributor