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Isotussilagine
Isotussilagine
ChemFaces products have been cited in many studies from excellent and top scientific journals
Product Name Isotussilagine
Price:
CAS No.: 91108-32-6
Catalog No.: CFN00273
Molecular Formula: C10H17NO3
Molecular Weight: 199.25 g/mol
Purity: >=98%
Type of Compound: Alkaloids
Physical Desc.: Powder
Source: The herbs of Echinacea purpurea.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Download: COA    MSDS
Similar structural: Comparison (Web)  (SDF)
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According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
Size /Price /Stock 10 mM * 1 mL in DMSO / Inquiry
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Product Use Citation
Related Screening Libraries
Size /Price /Stock 10 mM * 100 uL in DMSO / Inquiry / In-stock
10 mM * 1 mL in DMSO / Inquiry / In-stock
Related Libraries
Biological Activity
Description: Reference standards.
In vitro:
Planta Med. 1992 Dec;58(6):556-7.
Tussilagine and isotussilagine: two pyrrolizidine alkaloids in the genus arnica1.[Pubmed: 17226521]

METHODS AND RESULTS:
In flowerheads of ARNICA MONTANA, A. CHAMISSONIS ssp. FOLIOSA, A. AMPLEXICAULIS, and A. SACHALINENSIS, traces of the non-toxic pyrrolizidine alkaloids tussilagine and Isotussilagine were detected.
CONCLUSIONS:
Their identity was unambiguously confirmed by direct comparison (TLC, GC, GC/MS) with the authentic samples.
Isotussilagine Description
Source: The herbs of Echinacea purpurea.
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com
After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
More...

Cell. 2018 Jan 11;172(1-2):249-261.e12.
doi: 10.1016/j.cell.2017.12.019.
IF=36.216(2019)

PMID: 29328914

Cell Metab. 2020 Mar 3;31(3):534-548.e5.
doi: 10.1016/j.cmet.2020.01.002.
IF=22.415(2019)

PMID: 32004475

Mol Cell. 2017 Nov 16;68(4):673-685.e6.
doi: 10.1016/j.molcel.2017.10.022.
IF=14.548(2019)

PMID: 29149595

ACS Nano. 2018 Apr 24;12(4): 3385-3396.
doi: 10.1021/acsnano.7b08969.
IF=13.903(2019)

PMID: 29553709

Nature Plants. 2016 Dec 22;3: 16206.
doi: 10.1038/nplants.2016.205.
IF=13.297(2019)

PMID: 28005066

Sci Adv. 2018 Oct 24;4(10): eaat6994.
doi: 10.1126/sciadv.aat6994.
IF=12.804(2019)

PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 5.0188 mL 25.0941 mL 50.1882 mL 100.3764 mL 125.4705 mL
5 mM 1.0038 mL 5.0188 mL 10.0376 mL 20.0753 mL 25.0941 mL
10 mM 0.5019 mL 2.5094 mL 5.0188 mL 10.0376 mL 12.5471 mL
50 mM 0.1004 mL 0.5019 mL 1.0038 mL 2.0075 mL 2.5094 mL
100 mM 0.0502 mL 0.2509 mL 0.5019 mL 1.0038 mL 1.2547 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Structure Identification:
Tetrahedron Letters,1998,39(49): 9067–9068.
Enantioselective syntheses of tussilagine and isotussilagine[Reference: WebLink]

METHODS AND RESULTS:
Tussilagine and Isotussilagine are synthesized through the coupling reactions of N,N-disubstituted β-amino-esters with methyl pyruvate and Mitsunobu reactions as key steps.
CONCLUSIONS:
The first enantioselective syntheses of tussilagine and Isotussilagine via the coupling reactions of N,N-disubstituted β-amino esters with methyl pyruvate are described.
CFN00274Pyrrolam B126424-77-9155.20C8H13NO2
CFN00297Otonecine6887-34-9185.22C9H15NO3
CFN00308Chysin A22269-11-0169.22C9H15NO2
CFN00322Retronecine480-85-3155.20C8H13NO2
CFN00323Retronecine N-oxide6870-33-3171.2C8H13NO3
CFN00455Heliotridine520-63-8155.2C8H13NO2
CFN00456Heliotridine N-oxideN/A171.2C8H13NO3
CFN00341Australine118396-02-4189.21C8H15NO4
CFN00342Alexine116174-63-1189.21C8H15NO4
CFN002631,2-Epoxy-1-hydroxymethylpyrrolizidine15211-03-7155.20C8H13NO2
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