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Lycopene
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Product Name Lycopene
Price:
CAS No.: 502-65-8
Catalog No.: CFN98588
Molecular Formula: C40H56
Molecular Weight: 536.87 g/mol
Purity: >=98%
Type of Compound: Miscellaneous
Physical Desc.: Powder
Source: The fruits of Solanum lycopersicum
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
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Related Screening Libraries
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Biological Activity
Description: Lycopene is the pigment principally responsible for the characteristic deep-red color of ripe tomato fruits and tomato products. Lycopene is an antioxidant scavenger, hypolipaemic agent, inhibitor of pro-inflammatory and pro-thrombotic factors, thus potentially of benefit in cardiovascular disease (CVD). Lycopene may play a role in the prevention of Renal cell carcinoma, it also may play a role against obesity-related complications.
Targets: ROS | NADPH-oxidase | PI3K | Akt | Nrf2 | HO-1 | PPAR | Sirtuin
In vitro:
Clin Exp Pharmacol Physiol. 2015 Jun;42(6):632-9.
Lycopene inhibits cyclic strain-induced endothelin-1 expression through the suppression of reactive oxygen species generation and induction of heme oxygenase-1 in human umbilical vein endothelial cells.[Pubmed: 25932745]
Lycopene is the most potent active antioxidant among the major carotenoids, and its use has been associated with a reduced risk for cardiovascular disease (CVD). This study investigated the effects of Lycopene on cyclic strain-induced ET-1 gene expression in human umbilical vein endothelial cells (HUVECs) and identified the signal transduction pathways that are involved in this process.
METHODS AND RESULTS:
Cultured HUVECs were exposed to cyclic strain (20% in length, 1 Hz) in the presence or absence of Lycopene. Lycopene inhibited strain-induced ET-1 expression through the suppression of reactive oxygen species (ROS) generation through attenuation of p22(phox) mRNA expression and NAD(P)H oxidase activity. Furthermore, Lycopene inhibited strain-induced ET-1 secretion by reducing ROS-mediated extrace-llular signal-regulated kinase (ERK) phosphorylation. Conversely, Lycopene treatment enhanced heme oxygenase-1 (HO-1) gene expression through the activation of phosphoinositide 3-kinase (PI3K)/Akt pathway, followed by induction of the nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation; in addition, HO-1 silencing partially inhibited the repressive effects of Lycopene on strain-induced ET-1 expression.
CONCLUSIONS:
In summary, our study showed, for the first time, that Lycopene inhibits cyclic strain-induced ET-1 gene expression through the suppression of ROS generation and induction of HO-1 in HUVECs.Therefore, this study provides new valuable insight into the molecular pathways that may contribute to the proposed beneficial effects of Lycopene on the cardiovascular system.
Ultrason Sonochem. 2015 Nov;27:586-591.
Effect of high power low frequency ultrasound processing on the stability of lycopene.[Pubmed: 25921608]
The stability of Lycopene was evaluated after application of high power low frequency ultrasound.
METHODS AND RESULTS:
The study was carried out on a solution containing pure Lycopene to evaluate the direct effect of ultrasound on Lycopene and on tomato purée to evaluate the direct and indirect effects of ultrasound application within a food matrix. Power densities ranging from 55 to 5000 W/L and temperatures ranging from 23°C (ambient) to 60°C were evaluated. The experiments on pure Lycopene showed that the application of ultrasound did not have any direct effect over Lycopene. However, the retention of Lycopene in tomato puree has decreased indicating an indirect effect on Lycopene stability caused by high concentration of hydrogen peroxide and the activation of peroxidase enzymes leading to the reduction of ascorbic acid and its regenerative action towards Lycopene.
Curr Med Chem. 2011;18(8):1146-63.
Lycopene and cardiovascular diseases: an update.[Pubmed: 21291369]
Cardiovascular disease (CVD) is the leading cause of death in Western societies and accounts for up to a third of all deaths worldwide. In comparison to the Northern European or other Western countries, the Mediterranean area has lower rates of mortality from cardiovascular diseases and cancer, and this is attributed, at least in part, to the so-called Mediterranean diet, which is rich in plantderived bioactive phytochemicals. Identification of the active constituents of the Mediterranean diet is therefore crucial to the formulation of appropriate dietary guidelines.
METHODS AND RESULTS:
Lycopene is a natural carotenoid found in tomato, an essential component of the Mediterranean diet, which, although belonging to the carotenoid family, does not have pro-vitamin A activity but many other biochemical functions as an antioxidant scavenger, hypolipaemic agent, inhibitor of pro-inflammatory and pro-thrombotic factors, thus potentially of benefit in CVD. In particular, the review intends to conduct a systematic analysis of the literature (epidemiological studies and interventional trials) in order to critically evaluate the association between Lycopene (or tomato products) supplementation and cardiovascular diseases and/or cardiovascular disease risk factors progression, and to prepare provision of evidence-based guidelines for patients and clinicians. Several reports have appeared in support of the role of Lycopene in the prevention of CVD, mostly based on epidemiological studies showing a dose-response relationship between Lycopene and CVD. A less clear and more complex picture emerges from the interventional trials, where several works have reported conflicting results.
CONCLUSIONS:
Although many aspects of Lycopene in vivo metabolism, functions and clinical indications remain to be clarified, supplementation of low doses of Lycopene has been already suggested as a preventive measure for contrasting and ameliorating many aspects of CVD.
2016 Jul;37(7):9375-85.
Lycopene acts through inhibition of IκB kinase to suppress NF-κB signaling in human prostate and breast cancer cells[Pubmed: 26779636]
We studied the effect of the potent dietary antioxidant Lycopene on multiple points along the nuclear factor kappa B (NF-κB) signaling pathway in prostate and breast cancer cells. Lycopene significantly inhibited prostate and breast cancer cell growth at physiologically relevant concentrations of ≥1.25 μM. Similar concentrations also caused a 30-40 % reduction in inhibitor of kappa B (IκB) phosphorylation in the cells, as determined by western blotting. Furthermore, the same degree of inhibition by Lycopene was observed for NF-κB transcriptional activity, as determined by reporter gene assay. Concomitant with this, immunofluorescence staining of Lycopene-treated cells showed a significant suppression (≥25 %) of TNF-induced NF-κB p65 subunit nuclear translocation. Further probing of Lycopene's effects on upstream elements of the NF-κB pathway showed a 25 % inhibition of both activity of recombinant IκB kinase β (IKKβ) kinase in a cell-free in vitro assay, as well as activity of IKKβ immunoprecipitated from MDA-MB-231 cells treated with Lycopene. In conclusion, the anticancer properties of Lycopene may occur through inhibition of the NF-κB signaling pathway, beginning at the early stage of cytoplasmic IKK kinase activity, which then leads to reduced NF-κB-responsive gene regulation. Furthermore, these effects in cancer cells were observed at concentrations of Lycopene that are relevant and achievable in vivo. Keywords: Breast cancer; IκB kinase; Lycopene; NF-κB; Nutrition; Prostate cancer.
2016 Aug 17;56(11):1868-79.
Lycopene and Its Antioxidant Role in the Prevention of Cardiovascular Diseases-A Critical Review[Pubmed: 25675359]
The present review is based mainly on papers published between 2000 and 2011 and gives information about the properties of the carotenoid Lycopene in chemical and biological systems and its possible role in preventing cardiovascular diseases (CVD). The main aim of this report is to highlight its role as an antioxidant, also reported are bioactive properties that may influence the development of foam cells and protection against endothelial cell damage. The paper will also examine recent observations that Lycopene may improve blood flow and reduce inflammatory responses. Lycopene possesses antioxidant properties in vitro, and some epidemiological studies have reported protective effects against the progression of CVD. The oxidation of human low density lipoproteins (LDL) is a fundamental mechanism in the initiation of atherosclerosis. A beneficial role of Lycopene as antioxidant in the prevention of CVD is suggested but the data are still controversial. Lycopene is believed to be the most potent carotenoid antioxidant in vitro. Tissue culture experiments and animal studies support potential cardioprotective effects for Lycopene and other carotenoids in the blood. Most studies showed beneficial effects of Lycopene to individuals who are antioxidant-deficient like elderly patients, or humans exposed to higher levels of oxidative stress like smokers, diabetics, hemodialysis patients and acute myocardial infarction patients. By defining the right population and combining antioxidant potentials of Lycopene with vitamins and other bioactive plant compounds, the beneficial role of Lycopene in CVD can be clarified in future studies. Keywords: Atherosclerosis; LDL oxidation; in vitro; in vivo; isomerization.
In vivo:
2017 Feb 1;263:7-17.
Lycopene inhibits reactive oxygen species production in SK-Hep-1 cells and attenuates acetaminophen-induced liver injury in C57BL/6 mice[Pubmed: 27989599]
Our aim was to investigate the antioxidant potential of Lycopene in different experimental liver models: in vitro, to evaluate the influence of Lycopene on reactive oxygen species (ROS) production mediated by the PKC pathway and in vivo, to evaluate the protective effects of Lycopene in an experimental model of hepatotoxicity. The in vitro study assessed the Lycopene antioxidant potential by the quantification of ROS production in SK-Hep-1 cells unstimulated or stimulated by an activator of the PKC pathway. The role of NADPH oxidase was evaluated by measuring its inhibition potential using an inhibitor of this enzyme. In the in vivo study, male C57BL/6 mice received Lycopene (10 or 100 mg/kg by oral gavage) and 1 h later, acetaminophen (APAP) (500 mg/kg) was administrated. Lycopene decreased ROS production in SK-Hep-1 cells through inhibition of NADPH oxidase, brought about in the PKC pathway. Lycopene improved hepatotoxicity acting as an antioxidant, reduced GSSG and regulated tGSH and CAT levels, reduced oxidative damage primarily by decreasing protein carbonylation, promoted the downregulation of MMP-2 and reduced areas of necrosis improving the general appearance of the lesion in C57BL/6 mice. Lycopene is a natural compound that was able to inhibit the production of ROS in vitro and mitigate the damage caused by APAP overdose in vivo. Keywords: Acetaminophen; C57BL/6 mice; Hepatotoxicity; Lycopene; Oxidative stress; SK-Hep-1 cells. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.
Lycopene Description
Source: The fruits of Solanum lycopersicum
Solvent: Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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Calculate Dilution Ratios(Only for Reference)
1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 1.8626 mL 9.3132 mL 18.6265 mL 37.253 mL 46.5662 mL
5 mM 0.3725 mL 1.8626 mL 3.7253 mL 7.4506 mL 9.3132 mL
10 mM 0.1863 mL 0.9313 mL 1.8626 mL 3.7253 mL 4.6566 mL
50 mM 0.0373 mL 0.1863 mL 0.3725 mL 0.7451 mL 0.9313 mL
100 mM 0.0186 mL 0.0931 mL 0.1863 mL 0.3725 mL 0.4657 mL
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
Protocol
Animal Research:
Hum Exp Toxicol. 2015 Jun;34(6):612-9.
Lycopene-rich tomato oleoresin modulates plasma adiponectin concentration and mRNA levels of adiponectin, SIRT1, and FoxO1 in adipose tissue of obese rats.[Pubmed: 25632967]
To investigate whether Lycopene can modulate adiponectin levels and SIRT1 and FoxO1 gene expression in the adipose tissue of diet-induced obese rats.
METHODS AND RESULTS:
Male Wistar rats were first fed with hypercaloric diet (HD, n = 12) for 6 weeks, and afterward, these rats were randomly assigned to receive HD (n = 6) or HD with Lycopene-rich tomato oleoresin (equivalent to Lycopene 10 mg/kg body weight (BW)/day, HD + L, n = 6) by gavage for additional 6 weeks. Plasma Lycopene and adiponectin levels were analyzed by high-performance liquid chromatography and immunoassay, respectively. The messenger RNA (mRNA) expressions of adiponectin, Sirtuin 1 (SIRT1), Forkhead box O 1 (FoxO1), fatty acid translocase/cluster of differentiation 36 (FAT/CD36), and PPARγ in adipose tissues were determined by quantitative polymerase chain reaction. Lycopene was detected in the plasma of rats in HD + L group but not in the HD group. In addition, the Lycopene supplementation upregulated mRNA expressions of SIRT1, FoxO1, and FAT/CD36 but downregulated PPARγ in adipose tissue of obese rats.
CONCLUSIONS:
These data suggest that Lycopene, in the concentration used, is not toxic and also its health benefits in adipose tissue may play a role against obesity-related complications.
Arch Biochem Biophys. 2015 Apr 15;572:36-9.
Lycopene in the prevention of renal cell cancer in the TSC2 mutant Eker rat model.[Pubmed: 25602702]
Renal cell carcinoma (RCC) is the most frequent upper urinary tract cancer in humans and accounts for 80-85% of malignant renal tumors. Eker rat represents a unique animal model to study RCC since these rats develop spontaneous renal tumors and leiomyoma, which may be due to tuberous sclerosis 2 (TSC2) mutation resulting in the activation of the mammalian target of rapamycin (mTOR) pathway. This study examines the role of a Lycopene-rich diet in the development of RCC in the TSC2 mutant Eker rat model.
METHODS AND RESULTS:
Ten-week old female Eker rats (n=90) were assigned in equal numbers to receive 0, 100 or 200mg/kg of Lycopene as part of their daily diet. After 18 months the rats were sacrificed and the kidneys were removed. Immunohistochemical staining with antibodies against mTOR, phospho-S6 and EGFR were performed, as well as hematoxylin-eosin staining for histologic examination of the tumors. Tumors were counted and measured in individual kidneys. Presence of tumor decreased from 94% in control animals to 65% in the experimental group, but the difference was not statistically significant (P<0.12). However, mean numbers of renal carcinomas were statistically significantly decreased in the Lycopene-treated rats (P<0.008) when compared to untreated controls. In the Lycopene group, tumor numbers decreased (P<0.002) and the numbers tended to decrease linearly (P<0.003) as supplemental Lycopene increased from 0 to 200. Control rats fed only basal diet had a greater length of tumors (23.98 mm) than rats fed Lycopene supplement groups (12.90 mm and 11.07 mm) (P<0.05). Moreover tumor length decreased (P<0.02) and tumor length tended to decrease linearly (P<0.03) as supplemental Lycopene increased from 0 to 200mg/kg. All tumors showed strong staining with antibodies against mTOR, phospho-S6 and EGFR.
CONCLUSIONS:
In conclusion, dietary supplementation with Lycopene attenuates the development of renal cell cancers in the predisposed TSC2 mutant Eker rat model. These results suggest that Lycopene may play a role in the prevention of RCC.
Structure Identification:
J Agric Food Chem. 2015 Apr 1;63(12):3279-87.
Lycopene inhibits the isomerization of β-carotene during quenching of singlet oxygen and free radicals.[Pubmed: 25803572]
The present study aimed to investigate the influence of singlet oxygen and radical species on the isomerization of carotenoids.
METHODS AND RESULTS:
On the one hand, Lycopene and β-carotene standards were incubated with 1,4-dimethylnaphthalene-1,4-endoperoxide that produced singlet oxygen in situ. (13Z)- and (15Z)-β-carotene were preferentially generated at low concentrations of singlet oxygen, while high concentrations resulted in formation of (9Z)-β-carotene. The addition of different concentrations of Lycopene led to the same isomerization progress of β-carotene, but resulted in a decreased formation of (9Z)-β-carotene and retarded degradation of (all-E)-β-carotene. On the other hand, isomerization of β-carotene and Lycopene was induced by ABTS-radicals, too. As expected from the literature, chemical quenching was observed especially for Lycopene, while physical quenching was preferred for β-carotene. Mixtures of β-carotene and Lycopene resulted in a different isomerization progress compared to the separate β-carotene model. As long as Lycopene was present, almost no isomerization of β-carotene was triggered; after that, strong formation of (13Z)-, (9Z)-, and (15Z)-β-carotene was initiated.
CONCLUSIONS:
In summary, Lycopene protected β-carotene against isomerization during reactions with singlet oxygen and radicals. These findings can explain the pattern of carotenoid isomers analyzed in fruits and vegetables, where Lycopene containing samples showed higher (all-E)/(9Z)-β-carotene ratios, and also in in vivo samples such as human blood plasma.
Crit Rev Biotechnol. 2000;20(4):293-334.
Lycopene in tomatoes: chemical and physical properties affected by food processing.[Pubmed: 11192026 ]

METHODS AND RESULTS:
Lycopene is the pigment principally responsible for the characteristic deep-red color of ripe tomato fruits and tomato products. It has attracted attention due to its biological and physicochemical properties, especially related to its effects as a natural antioxidant. Although it has no provitamin A activity, Lycopene does exhibit a physical quenching rate constant with singlet oxygen almost twice as high as that of beta-carotene. This makes its presence in the diet of considerable interest. Increasing clinical evidence supports the role of Lycopene as a micronutrient with important health benefits, because it appears to provide protection against a broad range of epithelial cancers. Tomatoes and related tomato products are the major source of Lycopene compounds, and are also considered an important source of carotenoids in the human diet. Undesirable degradation of Lycopene not only affects the sensory quality of the final products, but also the health benefit of tomato-based foods for the human body. Lycopene in fresh tomato fruits occurs essentially in the all-trans configuration.
CONCLUSIONS:
The main causes of tomato Lycopene degradation during processing are isomerization and oxidation. Isomerization converts all-trans isomers to cis-isomers due to additional energy input and results in an unstable, energy-rich station.
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