ChemFaces is a professional high-purity natural products manufacturer.
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2. Pharmacological research
Citing Use of our Products
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According to end customer requirements, ChemFaces provide solvent format. This solvent format of product intended use: Signaling Inhibitors, Biological activities or Pharmacological activities.
|Size /Price /Stock
||10 mM * 1 mL in DMSO / $46.8 / In-stock||Other Packaging
||*Packaging according to customer requirements(100uL/well, 200uL/well and more), and Container use Storage Tube With Screw Cap
More articles cited ChemFaces products.
Food Science and Biotechnology...2015...J Drug Target.2016, 24:1-28Phytochemistry.2017, 141:162-170Korean Journal of Pharmacognosy...2017...Sci Rep. 2017, 8207(7)Anal Chim Acta.2018, 1039:162-171PLoS One.2018, 13(4):e0195642Evid Based Complement Alternat Me...2018...
Plant Cell Physiol.2018, 59(1):128-141Analytical methods2019, 11(6)Molecules.2019, 24(11):E2102Appl Biol Chem2019, 62:46Pharmacological Reports2020, 1-9Biochem Biophys Res Commun....2020...Nutrients.2020, 12(12):3638.LWT2020, 130:109535
Curr Top Med Chem....2020...Oxid Med Cell Longev....2020...Int J Mol Sci.2020, 21(24):9369.Chem Biol Interact.2020, 328:109200.Korean Herb. Med. Inf....2020...J of Food Quality2020, 8851285.Research Square2020, doi: 10.21203.
Our products had been exported to the following research institutions and universities, And still growing.
Universidade Federal de Santa C... (Brazil)Universidade Católica Portuguesa (Portugal)Istanbul University (Turkey)Vin?a Institute of Nuclear Scie... (Serbia)
Charles Sturt University (Denmark)Mahatma Gandhi University (India)Warszawski Uniwersytet Medyczny (Poland)Universidade Federal de Goias (... (Brazil)
Center for protein Engineering ... (Belgium)Kyung Hee University (Korea)Seoul National University (Korea)
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1 Building, No. 83, CheCheng Rd., Wuhan Economic and Technological Development Zone, Wuhan, Hubei 430056, PRC
Molecules.2019, 24(7):E1290Pharmacognosy Magazine2018, 14(56):418-424Nutrients.2019, 11(4):E936Phytomedicine.2018, 40:37-47Korean J of Pharmacognosy2020, 51,49-54.Sci. Rep.2015, 14-23Korean J Acupunct2020, 37:104-121Exp Mol Med.2020, 52(4):629-642.Research on Crops.2017, 18(3):569Analytical sci. & Tech2016, 186-193
Related Screening Libraries
||Phytolaccagenin has promising antifungal activity against ATCC standard cultures of Candida albicans and Cryptococcus neoformans, and against clinical isolates of these fungi, it also shows inhibitory effects on lipopolysaccharide-induced NO production, and haemolytic activities.|
||Antifection | NO|
|Nat. Prod. Commun.,2010, 5(7):1013-8. |
|Antifungal activity of saponin-rich extracts of Phytolacca dioica and of the sapogenins obtained through hydrolysis.[Pubmed: 20734930]|
METHODS AND RESULTS:
A saponin-rich extract of Phytolacca dioica L. berries, its acid hydrolysate, and its major aglycone, Phytolaccagenin, were assayed for antifungal activity against ATCC standard cultures of Candida albicans and Cryptococcus neoformans, and against clinical isolates of these fungi.
The activity of the extract was either low or negligible, but the hydrolysate, containing the sapogenins, including Phytolaccagenin, and also pure Phytolaccagenin, showed promising antifungal potency.
Hydrolysis of a natural product extract is shown to be a useful modification leading to improved bioactivity.
||The roots of Phytolacca acinosa Roxb
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)PMID: 29328914
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)PMID: 32004475
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)PMID: 29149595
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)PMID: 29553709
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)PMID: 28005066
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)PMID: 30417089
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
|J Pharm Biomed Anal. 2015 Mar 25;107:82-8. |
|Development and validation of a HPLC-MS/MS method for the determination of phytolaccagenin in rat plasma and application to a pharmacokinetic study.[Pubmed: 25575173]|
|Radix Phytolaccae (the dried root of Phytolacca acinosa Roxb. or Phytolacca americana L.) is widely used in East Asian countries for the treatment of inflammation-related diseases.
The active component of Radix Phtolaccae is Phytolcaccagenin a triterpenoid saponin. Phytolcaccagenin has anti-inflammatory activities that exceed those of Esculentoside A and its derivatives regarding suppression of LPS-induced inflammation, and has a lower toxicity profile with less hemolysis. To date, no information is available about analytical method and pharmacokinetic studies of Phytolaccagenin.
METHODS AND RESULTS:
To explore PK profile of this compound, a HPLC-MS/MS assay of Phytolaccagenin in rat plasma was developed and validated. The method was fully validated according to FDA Guidance for industry. The detection was performed by a triple-quadrupole tandem mass spectrometer with multiple reactions monitoring (MRM) in positive ion mode via electrospray ionization. The monitored transitions were m/z 533.2>515.3 for Phytolcaccagenin, and 491.2>473.2 for I.S. The analysis was performed on a Symmetry C18 column (4.6 mm × 50 mm, 3.5 μm) using gradient elution with the mobile phase consisting of acetonitrile and 0.1% formic acid water at a flow rate of 1 ml/min with a 1:1 splitter ratio. The method was validated with a LLOQ of 20 ng/ml and an ULOQ of 1000 ng/ml. The response versus concentration data were fitted with 1/x weighting and the correlation coefficient (r) were greater than 0.999. The average matrix effect and the average extraction recovery were acceptable. This validation in rat plasma demonstrated that Phytolaccagenin was stable for 30 days when stored below -20°C, for 6h at room temperature (RT, 22°C), for 12 h at RT for prepared control samples in auto-sampler vials, and during three successive freeze/thaw cycles results at -20°C.
The validated method has been successfully applied to an intravenous bolus pharmacokinetic study of Phytolaccagenin in male Sprague-Dawley rats (10 mg/kg, i.v.). Blood samples taken from 0 to 24h after injection were collected, and data analyzed with WinNonlin. The half-life and clearance were 1.4±0.9 h and 2.1±1.1 L/h/kg, respectively.