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    Sinapine thiocyanate
    Information
    CAS No. 7431-77-8 Price $100 / 20mg
    Catalog No.CFN90624Purity>=98%
    Molecular Weight368.45Type of CompoundPhenylpropanoids
    FormulaC16H24NO5.CNSPhysical DescriptionPowder
    Download Manual    COA    MSDSSimilar structuralComparison (Web)
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    Biological Activity
    Description: Sinapine thiocyanate has antioxidant activities and is hepatoprotective in a dose-dependent manner, implies that Brassica rapa seeds could be developed as a functional food for hepatoprotection.
    In vitro:
    《Food and Drug》 2014-03
    Stability of Sinapine Thiocyanate from Water Extract of Semen Raphani in Artificial Gastric and Intestinal Juice[Reference: WebLink]
    To investigate the stability of Sinapine thiocyanate in artificial gastric and intestinal juice gained from the water extract of Semen Raphani.
    METHODS AND RESULTS:
    HPLC was used to determine the area at different time points of Sinapine thiocyanate in artificial gastric juice with or without pepsin and artificial intestinal juice with or without pancreatin, the mass concentration and average remaining percentage were calculated respectively. Sinapine thiocyanate was more stable in artificial gastric juice than in artificial intestinal juice and most unstable in artificial intestinal juice without pancreatin. The stability of Sinapine thiocyanate was similar in two kinds of artificial gastric juice but considerable different in artificial intestinal juice.
    CONCLUSIONS:
    The remaining percentage of Sinapine thiocyanate in artificial gastric and intestinal juice, which were relatively stable, were all above 70 %, and that provided reference for the absorption mechanism of Sinapine thiocyanate.
    Sinapine thiocyanate Description
    Source: The seeds of Raphanus sativus L.
    Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 2.7141 mL 13.5704 mL 27.1407 mL 54.2814 mL 67.8518 mL
    5 mM 0.5428 mL 2.7141 mL 5.4281 mL 10.8563 mL 13.5704 mL
    10 mM 0.2714 mL 1.357 mL 2.7141 mL 5.4281 mL 6.7852 mL
    50 mM 0.0543 mL 0.2714 mL 0.5428 mL 1.0856 mL 1.357 mL
    100 mM 0.0271 mL 0.1357 mL 0.2714 mL 0.5428 mL 0.6785 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Protocol
    Structure Identification:
    《Strait Pharmaceutical Journal》 2013-03
    Determination of sinapine thiocyanate in YangHePingChuan granules by UPLC[Reference: WebLink]
    To establish a method for determination of Sinapine thiocyanate in YangHePingChuan granules by UPLC.
    METHODS AND RESULTS:
    UPLC method was adopted.The determination was performed on the Acquity UPLC BEH C18(100mm×2.1mm,1.7μm) column with mobile phase consisted of acetonitrile-0.08 mol·L-1 potassium dihydrogen phosphate(13∶ 87)at the flow rate of 0.25mL·min-1.The detection wavelength was set at 326nm and the column temperature was 30℃. The linear ranger of Sinapine thiocyanate was 0.105~1.05μg with an average recovery of 99.55%(RSD=1.82%,n=6).
    CONCLUSIONS:
    The method is accurate,reliable,simple and practical,it can be used for the quality control of granules.