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    CAS No. 20126-59-4 Price $318 / 20mg
    Catalog No.CFN98502Purity>=98%
    Molecular Weight462.41Type of CompoundFlavonoids
    FormulaC22H22O11Physical DescriptionYellow powder
    Download Manual    COA    MSDSSimilar structuralComparison (Web)
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    Biological Activity
    Description: Diosmetin-7-O-beta-D-glucopyranoside has antioxidant activity.
    In vitro:
    Zuckerindustrie., 2015, 140(10):632-9.
    An HPLC-DPPH method for antioxidant activity from sugarcane molasses.[Reference: WebLink]
    Sugarcane molasses is potentially rich in health-promoting phenolic compounds. Present study was designed to optimize experimental conditions for ultrasonic-assisted extraction of antioxidant compounds from sugarcane molasses using response surface methodology.
    An HPLCDPPH method for simultaneous determination of antioxidant activity was also developed. Ethanol concentration 80-84% (v/v), temperature 58-59 degrees C, and 47-50 min time duration were the most favorable conditions. In these conditions, the optimal experimental results were total phenolic content 18 mg gallic acid equivalents/g, with DPPH free radical scavenging ability of 92%, which was same as the predicted values by RSM model. Catechin, vanillic acid, isorhamnetin-3-O-glucoside, eugenol, schaftoside, Diosmetin-7-O-beta-D-glucopyranoside, ferulic acid, and caffeoylquinic acid were identified using HPLC-MS/MS. Among the antioxidant compounds, schaftoside was the most abundant antioxidant (92.081 mu g/g dry substance), while ferulic acid exhibited the highest antioxidant activity.
    These results suggest that HPLC-DPPH method is more specific, accurate and less time consuming and can sever as a quality control tool.
    Diosmetin-7-O-beta-D-glucopyranoside Description
    Source: The flowers of Chrysanthemum morifolium
    Solvent: DMSO, Pyridine, Methanol, Ethanol, etc.
    Storage: Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please email to: service@chemfaces.com

    After receiving: The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
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    Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.

    PMID: 29328914

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
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    doi: 10.3390/molecules22111829.

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    Calculate Dilution Ratios(Only for Reference)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 2.1626 mL 10.8129 mL 21.6258 mL 43.2517 mL 54.0646 mL
    5 mM 0.4325 mL 2.1626 mL 4.3252 mL 8.6503 mL 10.8129 mL
    10 mM 0.2163 mL 1.0813 mL 2.1626 mL 4.3252 mL 5.4065 mL
    50 mM 0.0433 mL 0.2163 mL 0.4325 mL 0.865 mL 1.0813 mL
    100 mM 0.0216 mL 0.1081 mL 0.2163 mL 0.4325 mL 0.5406 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    Structure Identification:
    J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Aug 15;965:150-7.
    An efficient preparative procedure for main flavonoids from the peel of Trichosanthes kirilowii Maxim. using polyamide resin followed by semi-preparative high performance liquid chromatography.[Pubmed: 25023212]
    In this study, a simple and efficient preparative procedure was developed for preparation of seven flavonoids from the peel of Trichosanthes kirilowii Maxim. using polyamide resin followed by semi-preparative high performance liquid chromatography (SPHPLC).
    First, the ethyl acetate fraction from the peel of T. kirilowii Maxim. obtained "prefractionation" using polyamide resin, which yielded two subfractions. And then the two subfractions were isolated by SPHPLC with an isocratic elution of methanol-water. Finally, seven known flavonoids were purified from 35 g of ethyl acetate extract including quercetin-3-O-[α-l-rhamnose (1→2)-β-d-glucopyranosyl]-5-O-β-d-glucopyranoside (19 mg), quercetin-3-O-rutinoside (24 mg), apigenin-7-O-β-d-glucopyranoside (10mg), diosmetin-7-O-β-d-glucopyranoside (Diosmetin-7-O-beta-D-glucopyranoside,45 mg), luteolin (21 mg), apigenin (15 mg), and diosmetin (56 mg). The purities of the compounds were determined by HPLC and the chemical structures were confirmed by UV and NMR analysis.
    In the present study, a simple, effective, and rapid procedure was established for preparative separation of multiple components from the peel of T. kirilowii Maxim. Furthermore, it was scalable and economical, so it was a promising basis for large-scale preparation of flavonoids from other plant extracts.