ChemFaces is a professional high-purity natural products manufacturer.
Product Intended Use
1. Reference standards
2. Pharmacological research
How to Order
Orders via your E-mail:
1. Product number / Name / CAS No.
2. Delivery address
3. Ordering/billing address
4. Contact information
Sent to Email: firstname.lastname@example.org
Order & Inquiry & Tech Support
Address: No. 83, CheCheng Rd., WETDZ, Wuhan, Hubei 430056, PRC
Delivery & Payment method
1. Usually delivery time: Next day delivery by 9:00 a.m. Order now
2. We accept: Wire transfer & Credit card & Paypal & Western Union
* Packaging according to customer requirements(5mg, 10mg, 20mg and more). We shipped via FedEx, DHL, UPS, EMS and others courier.
More articles cited ChemFaces products.
Biofactors.2017 Oct 24.Food Chem. 2017 Apr 15;Planta Med 2016 Apr 28.J Ethnopharmacol. 2017 Jul 12;BMC Complement Altern Med.2017 Aug 9;
Mol Immunol. 2016 Oct;78:121-132.Phytomedicine.2018 Jan 1;J Nat Med.2017 Jul 5. Food Analytical Methods07 April 2017RSC AdvancesIssue 86, 2017
Univ. of Limpopo2016Tropical J. of Pha. ResearchNo 3 (2017) Sci Rep. 2016 Apr 27Sci Rep. 2017 Jun 12;
Our products had been exported to the following research institutions and universities, And still growing.
Leibniz-Institut für Pflanzenbi... (Germany)Fraunhofer-Institut für Molekul... (Germany)Institute of Tropical Disease Un... (Indonesia)University of Limpopo (South Africa)
Kamphaengphet Rajabhat University (Thailand)University of Vigo (Spain)Rio de Janeiro State University (Brazil)Center for protein Engineering (... (Belgium)
Wageningen University (Netherlands)University of East Anglia (United Kingdom)Universitas Airlangga (Indonesia)
||Heterophyllin B is used as the quality control index for evaluating P. heterophylla in the Chinese Pharmacopoeia.Heterophyllin B effectively suppresses the adhesion and invasion of the human esophageal carcinoma cells by mediating the PI3K/AKT/β-catenin pathways and regulating the expression levels of adhesion- and invasion-associated genes.
||PI3K | Akt | Wnt/β-catenin | MMP(e.g.TIMP)|
|Mol Med Rep. 2016 Feb;13(2):1097-104. |
|Heterophyllin B inhibits the adhesion and invasion of ECA-109 human esophageal carcinoma cells by targeting PI3K/AKT/β-catenin signaling.[Pubmed: 26647768 ]|
|The present study aimed to measure the effect of Heterophyllin B (HB) on the adhesion and invasion of ECA-109 human esophageal carcinoma cells, and examine the possible mechanism involved. |
METHODS AND RESULTS:
A Cell Counting kit 8 assay was performed to determine the cell viability. Cell adhesion and invasion were determined following treatment of the ECA-109 cells with HB (0, 10, 25 and 50 μM) for 24 h. The levels of phosphorylated (p-)ATK and p-phosphoinositide 3-kinase (PI3K), and the protein levels of β-catenin were measured using western blot analysis. The mRNA and protein expression levels of E-cadherin, vimentin, snail, matrix metalloproteinase (MMP)2 and MMP9 were detected using reverse transcription-quantitative polymerase chain reaction and western blot analyses, respectively. HB (10, 25 and 50 μM) significantly suppressed the adhesion and invasion of the ECA-109 human esophageal carcinoma cells in a dose-dependant manner. The expression levels of p-ATK, p-PI3K and β-catenin were markedly decreased. The expression of E-cadherin was promoted, whereas the expression levels of snail, vimentin, MMP 2 and MMP 9 were decreased significantly in the ECA-109 cells treated with HB. In addition, HB inhibited the adhesion and invasion induced by PI3K activating peptide in the ECA-109 cells, and the protein expression levels were also adjusted.
These results suggested that HB effectively suppressed the adhesion and invasion of the human esophageal carcinoma cells by mediating the PI3K/AKT/β-catenin pathways and regulating the expression levels of adhesion- and invasion-associated genes.
Heterophyllin B Description
||The roots of Pseudostellaria heterophylla.
||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: email@example.com
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Recent ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.PMID: 29328914
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.PMID: 29149595
Scientific Reports 2017 Dec 11;7(1):17332.doi: 10.1038/s41598-017-17427-6.PMID: 29230013
Molecules. 2017 Oct 27;22(11). pii: E1829.doi: 10.3390/molecules22111829.PMID: 29077044
J Cell Biochem. 2018 Feb;119(2):2231-2239.doi: 10.1002/jcb.26385. PMID: 28857247
Phytomedicine. 2018 Feb 1;40:37-47. doi: 10.1016/j.phymed.2017.12.030.PMID: 29496173
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
|Biomed Chromatogr. 2015 Nov;29(11):1693-9. |
|LC-ESI-MS/MS analysis and pharmacokinetics of heterophyllin B, a cyclic octapeptide from Pseudostellaria heterophylla in rat plasma.[Pubmed: 25967583]|
|Heterophyllin B (HB) is a cyclic octapeptide isolated from Pseudostellaria heterophylla. Heterophyllin B is used as the quality control index for evaluating P. heterophylla in the Chinese Pharmacopoeia. |
METHODS AND RESULTS:
A rapid and sensitive LC-ESI-MS/MS method was developed and validated for the analysis of Heterophyllin B in rat plasma. Sample preparation consisted of a solid-phase extraction step for the removal of interference and preconcentration of the target analyte Heterophyllin B and the internal standard N-acetylcysteine before chromatographic analysis by MS/MS detection. The separation of Heterophyllin B and N-acetylcysteine was performed using a Hypersil GOLDTM C18 column and a mixture of methanol-water (60:40, v/v) containing 10 mmol/L ammonium formate and 0.1% formic acid as the mobile phase. The determination step was optimized in the selected reaction monitoring mode for the highly selective and sensitive quantitation of Heterophyllin B in rat plasma. Intra- and inter-assay precision (as relative standard deviation) was ≤9.1%, and accuracy was between 92.6 and 102.7%. The validated method was successfully applied to quantify Heterophyllin B concentrations up to 7 h after tail intravenous injections of 2.08, 4.16 and 8.32 mg/kg Heterophyllin B in rats.
The LC-MS/MS method identified the relevant pharmacokinetic parameters of Heterophyllin B and its studied analog.