|Source:||The roots of Sophora flavescens Ait.|
|Biological Activity or Inhibitors:|| 1. Kushenol C and kushenol A exhibit inhibitory activity against Sodium-dependent glucose cotransporter 2(SGLT2).
2. Kushenol C is a good 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenger.
3. Kushenol C shows antimicrobial activity against Staphylococcus aureus and Streptococcus mutans.
4. Kushenol C (IC(50) 5.45 microM) can inhibit beta-site APP cleaving enzyme 1 (BACE1) activities, it may be potent preventive and therapeutic candidates for Alzheimer's disease.
|Solvent:||Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc.|
|Storage:||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
|After receiving:||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.|
|1 mg||5 mg||10 mg||20 mg||25 mg|
|1 mM||2.2805 mL||11.4025 mL||22.805 mL||45.61 mL||57.0125 mL|
|5 mM||0.4561 mL||2.2805 mL||4.561 mL||9.122 mL||11.4025 mL|
|10 mM||0.2281 mL||1.1403 mL||2.2805 mL||4.561 mL||5.7013 mL|
|50 mM||0.0456 mL||0.2281 mL||0.4561 mL||0.9122 mL||1.1403 mL|
|100 mM||0.0228 mL||0.114 mL||0.2281 mL||0.4561 mL||0.5701 mL|
Biol. Pharm. Bull., 2008, 31(5):908-15.
|Re-evaluation of the antioxidant prenylated flavonoids from the roots of Sophora flavescens.[Pubmed: 18451517]|
|Upon re-examination of the ethyl acetate (EtOAc) soluble fraction of S. flavescens, two major prenylated chalcones, including kuraridin and kuraridinol, along with a minor prenylated flavonol, Kushenol C, were isolated as good DPPH scavengers. This was in contrast to the prenylated flavanones, sophoraflavanone G and kurarinone, which were isolated from the methylene chloride (CH(2)Cl(2)) fraction of the same source. Five flavanones consisting of kushenol E, leachianone G, kurarinol, sophoraflavanone G, and kurarinone exhibited significant antioxidant potentials in the ABTS, ONOO(-), and total ROS assays; however, the prenylated chalcones and prenylated flavonol showed more potent scavenging/inhibitory activities than the prenylated flavanones.|
Am. J. Chinese Med., 2012, 38(2):415-29.
|Selective inhibition of prenylated flavonoids from Sophora flavescens against BACE1 and cholinesterases.[Pubmed: 20387235 ]|
|It was previously reported that certain lavandulylated flavanones from Sophora flavescens are beta-site APP cleaving enzyme 1 (BACE1) inhibitors; however, based upon their levels within the extract, their inhibitory effects should be higher than expected. Moreover, chalcones and flavonols were reported to exert higher bioactivities than flavanones. These findings have led to a further search for other possible constituents potentially contributing to the strong inhibitory activity of the S. flavescens extract. In this study, BACE1 activities were significantly inhibited by 8-lavandulylkaempferol (IC(50) 7.29 microM), kuraridinol (IC(50) 7.10 microM), kuraridin (IC(50) 6.03 microM), and Kushenol C (IC(50) 5.45 microM) from the ethyl acetate fraction, along with desmethylanhydroicaritin (IC(50) 1.86 microM), xanthohumol (IC(50) 7.19 microM), and leachianone G (IC(50) 8.56 microM) from the dichloromethane fraction of the extract. The results indicate that the prenyl group, rather than the lavandulyl group, and the flavonols and chalcones, rather than flavanones, might make predominant contributions to BACE1 inhibition.|