ChemFaces is a professional high-purity natural products manufacturer.
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2. Pharmacological research
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More articles cited ChemFaces products.
Phytother Res.2015 Apr 17.Evid Based Complement Alternat Med. 2017:1583185Industrial Crops and Products.2015, P 185-191Molecules. 2017 Feb 8;J. Soc. Cosmet. Sci. KoreaJune 2016
Oncotarget. 2017 Nov 14;Phytother Res. 2016 Dec;Int J Mol Sci.2017 Nov 30;Acta ChromatographicaOct. 11, 2016Mol Med Rep.2015 Sep 21
Molecules 2016, 21(6)2016 Jun 22;21(6). Scientific Research.2015 Jan 6; 14-23Mediators Inflamm. 2016;2016:6189590.Oncol Rep.2015 Dec 22.
Our products had been exported to the following research institutions and universities, And still growing.
University of Dicle (Turkey)Nanjing University of Chinese Me... (China)Seoul National University (Korea)University of Queensland (Australia)
Aarhus University (Denmark)Periyar University (India)University of Toronto (Canada)Universidade Católica Portuguesa (Portugal)
Florida International University (USA)University of Liège (Belgium)Univerzita Karlova v Praze (Czech Republic)
||Polyphyllin G has been shown to have strong anticancer activities in a wide variety of human cancer cell lines, it also shows signiﬁcant anthelmintic activity against Dactylogyrus intermedius with EC50 values of 1.2 mg L(-1) and the acute toxicities (LC50) values of 2.9 mg L(-1).|
||Caspase | Bcl-2/Bax | Akt | ERK | JNK | p38MAPK | Autophagy | Antifection|
|Phytomedicine. 2016 Dec 1;23(13):1545-1554. |
|Polyphyllin G induces apoptosis and autophagy cell death in human oral cancer cells.[Pubmed: 27823618]|
|Polyphyllin G (also called polyphyllin VII), extract from rhizomes of Paris yunnanensis Franch, has been shown to have strong anticancer activities in a wide variety of human cancer cell lines. However, the underlying influences of autophagy in human oral squamous cell carcinoma (OSCC) remain unclear.
METHODS AND RESULTS:
In this study, the roles of apoptosis and autophagy in Polyphyllin G-induced death in human oral cancer cells were investigated. Moreover, the molecular mechanism of the anticancer effects of Polyphyllin G in human oral cancer cells was investigated.
The results revealed that Polyphyllin G significantly inhibited cell proliferation in human oral cancer cells; it dose-dependently induced apoptosis in SAS and OECM-1 cells through caspase-3, -8, and -9 activation and poly (ADP-ribose) polymerase cleavage. In addition, changes were observed in Bcl-2 and proapoptosis-related protein expression in different human oral cancer cell lines. The expression of both LC3-II and beclin-1 was markedly increased, suggesting the induction of autophagy in Polyphyllin G-treated oral cells. To further clarify whether Polyphyllin G-induced apoptosis and autophagy depended on Akt/extracellular signal-regulated kinases (ERK)/c-Jun N-terminal kinases (JNK)/p38 mitogen-activated protein kinases (MAPK) signaling pathways, the cells were cotreated with inhibitors. The results demonstrated Polyphyllin G-induced apoptosis in oral cells through the activation of ERK, Akt, p38 MAPK, and JNK, whereas ERK and JNK accounted for Polyphyllin G-induced autophagy.
This study is the first to demonstrate apoptosis and autophagy during Polyphyllin G-induced cell death in human oral cancer cell lines. These results suggest that Polyphyllin G is a promising candidate for developing antitumor drugs targeting human oral squamous cell carcinoma.
|Parasitology. 2013 Jul;140(8):952-8. |
|Identification of compounds from Paris polyphylla (ChongLou) active against Dactylogyrus intermedius.[Pubmed: 23552446]|
|The present study was designated to ascertain the anthelmintic activity of the rhizomes of Paris polyphylla and to isolate and characterize the active constituents.
METHODS AND RESULTS:
The methanol extract from rhizomes of P. polyphylla showed signiﬁcant anthelmintic activity against Dactylogyrus intermedius with the median effective concentration (EC50) 22.5 mg L(-1). Based on this finding, the methanol extract was fractionated by silica gel column chromatography in a bioassay-guided fractionation yielding 2 bioactive compounds, the structures of these compounds were elucidated as formosanin C and polyphyllin VII(Polyphyllin G). The in vivo tests revealed that formosanin C and polyphyllin VII were significantly effective against D. intermedius with EC50 values of 0.6 and 1.2 mg L(-1), respectively. The acute toxicities (LC50) of formosanin C and polyphyllin VII for grass carp were 2.8 and 2.9 mg L(-1), respectively.
The overall results provide important information for the potential application of formosanin C and polyphyllin VII in the therapy of serious infection caused by D. intermedius.
Polyphyllin G Description
||The rhizomes of Paris yunnanensis Franch.
||DMSO, Pyridine, Methanol, Ethanol, etc.
||Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 24 months(2-8C).
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20C. Generally, these will be useable for up to two weeks. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please email to: firstname.lastname@example.org
||The packaging of the product may have turned upside down during transportation, resulting in the natural compounds adhering to the neck or cap of the vial. take the vial out of its packaging and gently shake to let the compounds fall to the bottom of the vial. for liquid products, centrifuge at 200-500 RPM to gather the liquid at the bottom of the vial. try to avoid loss or contamination during handling.
Recent ChemFaces New Products and Compounds
Recently, ChemFaces products have been cited in many studies from excellent and top scientific journals
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.PMID: 29328914
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.PMID: 29149595
Scientific Reports 2017 Dec 11;7(1):17332.doi: 10.1038/s41598-017-17427-6.PMID: 29230013
Molecules. 2017 Oct 27;22(11). pii: E1829.doi: 10.3390/molecules22111829.PMID: 29077044
J Cell Biochem. 2018 Feb;119(2):2231-2239.doi: 10.1002/jcb.26385. PMID: 28857247
Phytomedicine. 2018 Feb 1;40:37-47. doi:10.1016/j.phymed.2017.12.030PMID: 29496173
Calculate Dilution Ratios(Only for Reference)
* Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
|Biomed Chromatogr. 2013 Mar;27(3):343-8 |
|Simultaneous determination and pharmacokinetic study of polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII (Polyphyllin G) in beagle dog plasma after oral administration of Rhizoma Paridis extracts by LC-MS-MS.[Pubmed: 22903625]|
|For the first time, a rapid and specific LC-MS-MS method has been developed for the analysis of polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII(Polyphyllin G) in beagle dog plasma.
METHODS AND RESULTS:
The method was applied to study the pharmacokinetics of Rhizoma Paridis extracts containing polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII. The analysis was carried out on an Agilent Zorbax XDB-C(18) reversed-phase column (100 × 2.1 mm, 1.8 μm) by isocratic elution with acetonitrile and water (50:50, v/v). The flow rate was 0.25 mL/min. All analytes including internal standards were monitored by selected reaction monitoring with an electrospray ionization source. Linear responses were obtained for polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII ranging from 10 to 5000 ng/mL. The intra-and inter-day precisions (RSDs) were less than 6.66 and 9.15%. The extraction recovery ranged from 95.53 to 104.21% with RSD less than 8.69%. Stability studies showed that polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII were stable in preparation and analytical process.
The validated method was successfully used to determine the concentration-time profiles of polyphyllin I, polyphyllin II, polyphyllin VI and polyphyllin VII.